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Journal of Experimental Hematology ; (6): 537-541, 2007.
Artículo en Chino | WPRIM | ID: wpr-276877

RESUMEN

The purpose of this study was to synthesize the complex of magnetic nanoparticles and antibody, and to apply them to isolate the CD34(+) cells from umbilical cord blood, then to evaluate its separation efficiency. The complex of magnetic nanoparticles and antibody was used to separate CD34(+) cells from umbilical cord blood in the outer magnetic field because of its superparamagnetism, specific identification and function of combination with CD34(+) cells. Scanning electron microscopy was used to observe the morphology of the separated CD34(+) cells. Flow Cytometer was applied to evaluate the sorting efficiency of magnetic nanoparticles, liquid culture and colony culture were taken to assay proliferation and differentiation capacity of the separated CD34(+) cells. The results showed that the CD34(+) cells from umbilical cord blood were isolated fast and effectively by the complex of magnetic nanoparticles and monoclonal antibody. Moreover, the isolated CD34(+) cells still maintained its normal morphology, highly proliferative and differentiative capacity. It is concluded that the complex of magnetic nanoparticles and monoclonal antibody has been successfully synthesized and developed as a technique which efficiently and quickly isolates CD34(+) cells from umbilical cord blood.


Asunto(s)
Humanos , Antígenos CD34 , Metabolismo , Sangre Fetal , Biología Celular , Citometría de Flujo , Células Madre Hematopoyéticas , Biología Celular , Alergia e Inmunología , Separación Inmunomagnética , Métodos , Magnetismo , Nanopartículas
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