Measurement and analysis of implant-related anatomical structure in the adult mandibular posterior tooth area based on CBCT / 安徽医科大学学报

Objective @#To measure and analyze the position and shape of implant-related anatomical structure such as mental foramen，mental canal and the submandibular fossa in the adult mandibular posterior tooth area based on Cone Beam Computed Tomography( CBCT) images，and to detect the relationship between anatomical structures and the anatomice buccalingual angulation at implant site in order to help determine the implant angulation at the implant treatment-planning phase．@*Methods@#Random samples of 181 CBCT images were selected and analyzed，including 109 patients with dentition defect : 59 males and 50 females，and 72 patients with non dentition defect : 36 males and 36 females．The anatomic marks and alveolar bone inclination of mandibular posterior tooth area were measured respectively，and the results were statistically analyzed. @*Results @#The inclination angles of alveolar bone were (5. 08 ± 3. 86) on the left and (3. 07 ± 5. 20) on the right of the mental foramen area，(8. 57 ± 5. 03) and (9. 54 ± 5. 19) on the first mandibular molar，( 15. 48 ± 5. 23) and ( 14. 21 ± 6. 67) on the second mandibular molar，and the inclination angles of the left and right mental canal were (41. 81 ± 10. 07) and (44. 77 ± 8. 96) respectively．The distance from the upper edge of the mental foramen to the top of the alveolar ridge was ( 12. 35 ± 2. 38) mm，and the distance between the mental canal at 1．5 mm inside the mental foramen and the top of the alveolar ridge was ( 14. 14 ± 2. 46) mm．The depth of the submandibular gland fossa in the first and second molars were (0. 80 ± 0. 56) mm and ( 1. 00 ± 0. 50) mm，the distance from the most concave point to the alveolar crest top were ( 14. 15 ± 5. 95) mm and ( 14. 52 ± 3. 55) mm，the distance from the most convex point of the crown to the alveolar crest top were ( 10. 71 ± 4. 72) mm and (9. 25 ± 2. 81) mm，and the distance from the upper edge of the mandibular canal to the alveolar crest top were ( 15. 52 ± 3. 48) mm and ( 13. 83 ± 2. 76) mm．@*Conclusion@#The buccallingual inclination of mandibular alveolar bone is gradually inclined from buccal side to lingual side from premolar area to molar area，and the lingual inclination in the second molar area is the most obvious ; The implant tip can stop at the upper edge of the mental foramen to obtain better initial stability ; The existence of submandibular fossa limits the depth and direction of implant implantation to a certain extent．It is necessary to accurately measure and analyze this part with CBCT before operation to avoid complications such as mandibular lingual perforation and improve the success rate of implant repair.

Phage display peptide library for screening the peptides that specifically bind to osteoblasts cells / 中华口腔医学杂志

<p><b>OBJECTIVE</b>To explore the experimental methods that the phage peptide library technology screening human osteoblast specificity polypeptide, which will provide the basis of the experiment of the Ti surface biolization modification.</p><p><b>METHODS</b>Human calvarial osteoblasts were used as the target cells for whole-cell biopanning from a 12-mer peptide phage-display library. Cell eluent and cell lysis buffer were cultivate and count respectively after washing. Then the target cells were analyzed by enzyme-linked immunosorbent assay (ELISA) and immunofluorescence detection to authenticate the positive phage clones by human gingival fibroblast as the absorber cells. The positive phage clones were deduced by DNA sequencing.</p><p><b>RESULTS</b>After four rounds of screening, twenty-two positive phage clones were found out from randomly selected phage monoclonals, whose single-strand DNA were extracted and sequenced. Amino acid sequence of the highest frequency peptide was MGWSWWPETWPM.</p><p><b>CONCLUSIONS</b>The specific peptide against human osteoblasts can be obtained from a phage-display peptide library for use as a new research approach and experimental basis of the biolization modification of the titanium surface.</p>

Correlation between liver stiffness measured by shear-wave elasticity imaging and pathological grades and stages of chronic hepatitis B / 中华传染病杂志

Objective To investigate the correlation between Young′s elastic modulus (EI) using shear wave elastography (SWE) and liver pathology .Methods Liver biopsy was performed on 231 patients with chronic hepatitis B (CHB) under supersonic guidance ,and SWE with EI of liver was obtained concurrently .The correlation between measured liver stiffness and pathology was analyzed by using the liver pathology as golden standards .One‐way analysis of variance and Spearman rank correlation analysis were performed for the comparison between groups and correlation between two variables , respectively .Receiver operating characteristic (ROC) curve was used to explore the predictive value of shear modulus for the liver inflammation grades and fibrosis stages .Results The EI medians of different liver inflammation grades were 6 .78 kPa (G1) ,7 .30 kPa (G2) ,9 .93 kPa (G3) and 14 .93 kPa (G4) , respectively ,which were statistically different (H=55 .19 ,P<0 .01) .And EI medians of various fibrosis stages were 6 .62 kPa (S0 -S1) ,7 .15 kPa (S2) ,9 .78 kPa (S3) and 14 .62 kPa (S4) ,respectively , which were also significantly different (H=62 .14 ,P<0 .01) .EI was positively correlated with both liver inflammation grades (r=0 .454 6 ,P<0 .01) and liver fibrosis stages (r=0 .505 6 ,P<0 .01) .The areas under the ROC for G≥2 ,G≥3 and G=4 were 0 .68 (95% CI:0 .61 -0 .75) ,0 .77 (95% CI:0 .70 -0 .84) and 0 .85 (95% CI:0 .77-0 .92) ,respectively .The areas under the ROC for S≥2 ,S≥3 and S=4 w ere 0 .73 (95% C I:0 .66 -0 .79 ) ,0 .78 (95% C I:0 .72 -0 .85 ) and 0 .83 (95% C I:0 .75 -0 .91 ) , respectively .Conclusion The EI measured by SWE is correlated with liver pathology of CHB patients , which may be used to dynamically monitor the progress of liver fibrosis .

Moxonidine-induced transient pressor response is mediated by both I1-imidazoline receptors and α2-adrenoceptors in anesthetized spontaneously hypertensive rats / 第二军医大学学报

Objective:Clonidine,by activating peripheral α-sbrenoceptors, produces transient pressor response after i.v.injection in anesthetized animals.Moxonidine, with at least 40-fold higher affinity to I1-imidazoline receptors than to α2-adrenoceptors,produces also a transient pressor response. This work was designed to investigate whether I1-imidazoline receptors are involved in this pressor effect of moxonidine. Methods:Female spontaneously hypertensive rats(SHRs,aged 14-16 weeks)were anesthetized with urethane.To observe the transient pressor responses,moxonidine 0.1,0.3,1.0mg/kg(intravenous,i.v),2.0μg(intracerebroventricular,i.c.v.)and 1.0,10.0mg/kg(intragastric,i.g.)were administrated in different groups of rats.To evaluate the roles of α1-adrenoceptors,α2-adrenoceptors and I1-imidazoline receptors in the transient pressor responses to moxonidine, prazosin(10.0μg/kg),yohimbine(2.0mg/kg),phentolamine(0.2mg/kg),idazoxan(1.0mg/kg)or yohimbine+idazoxan(2.0mg/kg+1.0mg/kg)were intravenously given to the animals before moxonidine 0.3mg/kg (i.v.).Results:It was found that i.v.moxonidine produced a greater pressor response than clonidine when producing a similar reduction of blood pressure.This effect of moxonidine was not influenced by prazosin, but was partly inhibited by yohimbine, phentolamine or idazoxan,and completely blocked by the combination of yohimbine and idzaxon.Neither i.c.v.injection nor i.g. administration of moxonidine induced transient pressor responses.Conclusion:The transient pressor response of i.v. moxonidine is mediated by both peripheral I1-imidazoline receptors and α2-adrenoceptors.