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Objective To preliminarily explore the influence of intervention of atorvastatin in vasculogenic mimicry of glioblastoma and its mechanism.Methods CCK-8 experiment was carried out to detect the cell activity of glioma cell line U87 under the intervention of atorvastatin at different concentrations (1010-10-4 mol/L),aiming at screening out the safety concentration of atorvastatin.U87 cells were incubated in the mediums containing atorvastatin with different safety concentrations as well as in the control medium without atorvastatin.Three-D cultivation was performed in the Matrgel to establish the in vitro vasculogenic mimicry models,then the ability ofvasculogenic mimicry was observed and the length of the tube structure was counted.RT-PCR and Western blotting were performed to detect the mRNA and protein expression levels of matrix metalloproteinase-2 (MMP-2) in the U87 cells of intervention group and control group,respectively.Results Safety concentrations of atorvastatin screened by CCK-8 experiment were 10-7,10-6 and 10-5 mol/L.U87 cells formed the typical vasculogenic mimicry tube structure at 6-8 h after inoculation; the number of tube structure decreased with the increase ofatorvastatin concentration.Compared with the control group,the length counting of tube structure in intervention groups (10-7,10-6 and 10-5 mol/L) was significantly reduced and the mRNA and protein expression levels of MMP-2 were markedly decreased (P＜0.05).Conclusion Atovastatin significantly inhibits the vasculogenic mimicry formation via down-regulating the MMP-2 expression level.
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Objective To study the effect of low frequency stimulation (LFS) in the pedunculopontine tegmental nucleus (PPTg) on gait and movement ability in unilateral lesioned Parkinson's disease(PD) rats.Methods Thirty-two male SD rats were randomly assigned to control group (6-hydroxydopamine treatment,n=8) and experimental group (6-hydroxydopamine treatment combined with electrode implantation,n=24).All subjects were processed with injection of 6-hydroxydopamine in the fight medial forebrain bundle (MFB) and simultaneously the rats in the experimental group were received an electrode implantation into the ipsilateral PPTg.Catwalk gait and open field test data were acquired at 1 week before stereotactic surgery and 3 weeks after operation in the rats of control and experimental groups; the behavioral data of rats in the experimental group were recorded again 4 weeks after operation when they were under ongoing LFS-PPTg.Results Catwalk results showed that the postoperative couplings values (right front→left front,right front→left hind,right hind →left front,right hind →left hind) were significantly increased in all subjects as compared with the preoperative ones (P＜0.05); while couplings value in rats of the experimental group after LFS-PPTg significantly reduced as compared with that before LFS-PPTg (P＜0.05).The postoperative run speed variation statistically increased and postoperative cadence significantly decreased in all rats as compared with the preoperative ones (P＜0.05); the run speed variation significantly decreased and cadence obviously increased in experimental group after LFS-PPTg as compared with those before LFS-PPTg (P＜0.05).Open field test:the moving distance of all subjects decreased significantly after operation; the distance in rats of the experimental group after LFS-PPTg increased significantly as compared with that before LFS-PPTg.Conclusion LFS-PPTg can effectively improve gait function and motor activity of unilateral PD rat models.
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<p><b>OBJECTIVE</b>To discover and identify differentially expressed genes associated with colorectal adenoma formation and the role of RegIV in colorectal adenoma differentiation.</p><p><b>METHODS</b>A subtracted cDNA library was constructed with cDNAs that were isolated from either the normal mucosa or adenoma tissue of a single patient. Suppressive subtractive hybridization (SSH) combined with virtual northern blotting was used to characterize differentially expressed genes and contigs were assembled by electronic cloning (in silico cloning) with the EST database. Semi-quantitative RT-PCR was performed in 9 colorectal adenomas.</p><p><b>RESULTS</b>The amino acid sequence was determined with open reading frame (ORF) prediction software and was found to be 100% homologous to the protein product of RegIV (a novel gene isolated from a large inflammatory bowel disease library). RegIV was found to be highly expressed in all of the adenoma samples (9/9) compared with the normal mucosa samples, while 5/6 cases showed RegIV to be more strongly expressed in adenocarcinoma.</p><p><b>CONCLUSION</b>RegIV may play an important role in the initiation of colorectal adenoma differentiation, and its detection may be useful in the early diagnosis of colorectal adenoma formation.</p>