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1.
Journal of the Egyptian Society of Parasitology. 2004; 34 (3): 967-78
en Inglés | IMEMR | ID: emr-66788

RESUMEN

To compare the immunologic techniques with the conventional staining methods [mainly modified Ziehl-Neelsen, MZN], 93 children [65 immunocompromised and 28 immunocompetent] potentially at risk of Cryptosporidium parvum were studied. Using MZN, a prevalence of 10.7% in diarrheic children was found. ELISA coproantigen and detection of 23 kDa band of immunoblotting by serum IgG were sensitive and specific. They gave 85.7% sensitivity, 100% specificity, 100% diagnostic accuracy, 100% positive predictive value and 98.9% negative predictive value. ELISA detection of serum IgG gave 85.7% sensitivity, 97.7% specificity, 96.8% diagnostic accuracy, 75% positive predictive value and 98.9% negative predictive value. So, it was concluded that 23 kDa band determined by ELISA is a valuable sensitive and specific mean of diagnosing cryptosporidiosis, as this antigen is a consistent target of the humoral immune response


Asunto(s)
Humanos , Masculino , Femenino , Diarrea , Antígenos de Protozoos , Ensayo de Inmunoadsorción Enzimática , Microscopía , Sensibilidad y Especificidad , Inmunoglobulina G , Prevalencia , Niño
2.
Journal of the Egyptian Society of Parasitology. 1993; 23 (2): 555-61
en Inglés | IMEMR | ID: emr-28403

RESUMEN

A comparative study of Kato thick smear method, modified Ritchie concentration technique [MRCT], rectal snip biopsy and indirect hemagglutination [IHA] test were done on 50 patients with chronic intestinal schistosomiasis from Monoufia Governorate, an area of Nile Delta endemic for schistosomiasis. Study revealed that a single Kato-preparation was not sufficient to determine correctly the prevalence of Schistosoma mansoni infection particularly in chronic cases [22%], the accuracy was maximized by examining stool samples obtained from the same patient by MRCT [46%]. Rectal snip biopsy and IHA test showed high positivity rate of 80% and 74%, respectively. It is recommended to combine stool examination by MRCT with either rectal snip or IHA test according to the condition of patient as the former can not be performed on routine basis


Asunto(s)
Pruebas Diagnósticas de Rutina/métodos , Esquistosomiasis/epidemiología , Estudio de Evaluación/métodos , Serología
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