Immunohistochemical study of schistosomal soluble egg antigen in male genital organs of schistosomiasis mansoni infected mice

In this study, 70 Swiss outbred mice were used for the demonstration of circulating soluble egg antigen [CSEA] in serum and testes of S. mansoni infected mice and to study the possible role of this antigen [CSEA] in the pathogenesis of reproductive organ lesions using two recently described monoclonal antibodies to repetitive carbohydrate epitopes of S. mansoni SEA. Ten mice as a control group and the other 60 mice were infected with 100 S. Mansoni cercariae per mouse by subcutaneous injection. One group was sacrificed in each of 6, 8, 10, 12, 14 and 16 weeks post-infection [PI], while the control mice were killed at one time. The sacrificed mice were bled and their sera were used for detecting CSEA by ELISA. The mean level of CSEA in the serum of infected mice started to appear six weeks PI and gradually increased till 16 weeks PI. This was coincided with the appearance of the fluorescent deposits in blood vessels of testis at eight weeks PI and later in the stroma of testis from the 10th week onwards

Assessment of the therapeutic efficacy of praziquantel on the colonic lesion in experimentally infected mice with schistosoma mansoni

Therapeutic efficacy of praziquantel on colonic schistosomiasis was assessed in this study. Sixty swiss albino mice were infected by 80-100 Schistosoma mansoni cercariae and classified into four groups these first group received a single oral dose of 6.24 mg praziquantel/mouse, the second group was treated by the same dose daily in two successive days, the third group was treated also by the same dose in three successive days and the fourth group was left untreated as a control group. It was found that the third group which was treated by the triple doses give a higher cure rate [98%] than the other two treated groups [64.7% and 82.7% respectively]. Histopathological examination of the colon showed no definite pathological changes in the third group in comparison with the other groups. These data indicate that treatment by the triple doses of praziquantel on three successive days gives a high cure rate of the patients with less side effects

Immunohistopathological status of the skin in cases infested with three species of mites

This study was performed on 80 patients with clinical manifestations of scabies. Sarcoptes scabiei adult or larva was isolated from 39 patients [48.8%]. Dermanyssus gallinae [red poultry mite] and Dermatophagoides sp. were isolated from 6 and 5 patients, respectively. Punch biopsies were taken from the patients, stained with hematoxylin and eosin and direct immunofluorescence stain to study the immunopathological status of the skin infested with 3 species of mites. The results declared that the pathological changes and the immunoglobulin deposition in the skin were nearly similar, regardless of the type of the causative mite, except of the presence of burrows in Sarcoptes scabiei

Immunological and histopathological study on splenectomized mice infected with giardia lamblia

This study was carried out on 60 mice allocated in 2 groups. Group I included 30 mice which were splenectomized and orally inoculated with Giardia lamblia cysts. Group II [30 intact infected mice] which served as a control group. The mice in group I captured G. lamblia infection and demonstrated trophozoites in their intestinal sections when PAS [periodic acid Schiff] and IIF [indirect immunofluorescence] stains. On the other hand, intestinal sections stained with hematoxyline and eosin showed a spectrum of pathological changes with shortened fused villi, heavy lymphocytic infiltration in the lamina propria and atrophy in musculosa and ganglion cells which showed decrease in number per base of villus. The levels of antibody response measured with IIF-test showed significant decrease in group I compared with the control group. This study demonstrated the role of humoral immunity in infection with G. lamblia