Comparing the effect of silybin and silybin advanced[TM] on viability and HER2 expression on the human breast cancer SKBR3 cell line by no serum starvation

The polyphenol silybin has anti-oxidant and anti-cancer properties. The poor bioavailability of some polyphenols [flavonoids, and terpenoids] can be improved by binding them to phosphatidylcholine [phytosome technology]. Many studies have focused on the most common phytosome, silybin-phosphatidylcholine, particularly for its hepatoprotective effects. However, in recent years, studies have also been conducted to determine its anti-cancer effect. Considering that the serum starvation should not be used for studies that are not focused on cell cycle arrest, we studied the effect of silybin-phosphatidylcholine from Silybin Advanced[TM] in 1:2 ratio [one part silybin bound to two parts phosphatidylcholine] on HER2 gene expression on the SKBR3 breast cancer cell line which were cultured in complete medium [not serum deprivation]. The results were compared with our previous study of silybin on HER2 expression on SKBR3 cells. An MTT test was used to determine concentrations for cell treatment, and the gene expression was defined by real-time RT-PCR. Outcomes showed significant concentration- and time-dependent cell growth inhibitory effects of silybin, and silybin-phosphatidylcholine and HER2 down regulation on SKBR3 cells. Silybin-phosphatidylcholine concentrations had a much larger inhibitory and HER2 down regulate effect on cell growth than the same silybin concentrations on SKBR3 cells

comparison of the effects of silybin and silybin-phosphatidylcholine on viability and ESR expression in human breast cancer T47D cell line

Silybin is a polyphenol with anti-oxidant and anti-cancer properties. The poor bioavailability of some polyphenols can be improved by binding to phosphatidylcholine. In recent years, studies have been conducted to evaluate the anti-cancer effect of silybin. We studied the effect of silybin and silybin-phosphatidylcholine on ESR1 and ESR2 gene expression and viability in the T47D breast cancer cell line. In this experimental study, a 3-[4,5-Dimethylthiazol-2-Yl]-2,5- Diphenyltetrazolium Bromide test [MTT test] was used to determine doses for cell treatment, and the gene expression was analyzed by real-time reverse transcriptase-polymerase chain reaction [real-time RT- PCR]. Significant dose- and time-dependent cell growth inhibitory effects of silybin and silybin-phosphatidylcholine along with ESR1 down-regulation were observed in T47D cells. In contrast to ESR1, the T47D cell line showed negligible ESR2 expression. This study suggests that silybin and silybin-phosphatidylcholine down-regulate ESR1 in ER+ breast cancers. Results also show that in the T47D cell line, silybinphosphatidylcholine has a much higher growth inhibitory effect and a more significant down-regulation of ESR1 compared with silybin