Probiotic and synbiotic in broiler diet: performance and Enterobacteriaceae / [Probiótico e simbiótico em ração para frangos: desempenho e Enterobacteriaceae]

The objective of this study was to evaluate the effects of probiotics and synbiotics on the performance and Enterobacteriaceae count of broiler chickens. A total of 640 one-day-old male broiler chicks were distributed in a completely randomized design with four treatments and eight replicates with 20 birds each. The treatments were: ration with performance enhancer (zinc bacitracin; positive control); ration without performance enhancer and probiotic/synbiotic (negative control); ration with probiotics; and ration with synbiotics. At 35 days, five birds from each treatment were euthanized and intestinal contents were harvested for determining the Enterobacteriaceae count. The performance data and average colony-forming units (CFUs) transformed as log CFU/g were subjected to analysis of variance and Tukey's test. The effects of probiotics and synbiotics were observed in the initial phase, with supplemented birds exhibiting comparable weight gain to those supplemented with bacitracin. No effect of the treatment on broiler performance was observed after 42 days. The enterobacterial count was comparable among all experimental treatments. Supplementation with probiotics and synbiotics did not compromise the performance of broilers and did not alter the Enterobacteriaceae count.(AU)

Objetivou-se avaliar o efeito do probiótico e do simbiótico sobre o desempenho e a contagem de Enterobacteriaceae em frangos. Foram utilizados 640 pintos de corte, machos, de um dia de idade, distribuídos em delineamento inteiramente ao acaso, com quatro tratamentos, oito repetições com 20 aves cada. Os tratamentos foram: ração com melhorador de desempenho (bacitracina de zinco) (controle positivo); ração sem melhorador de desempenho e sem probiótico/simbiótico (controle negativo); ração com probiótico e ração com simbiótico. Aos 35 dias, cinco aves por tratamento foram eutanasiadas para retirada de conteúdo intestinal e determinação de Enterobacteriaceae. As médias das unidades formadoras de colônias, transformadas em log/UFC/g, e de desempenho foram submetidas à análise de variância e comparadas pelo teste Tukey. Foi observado efeito do probiótico e do simbiótico na fase inicial, sendo que aves apresentaram os mesmos resultados de ganho de peso e de peso corporal que o grupo de aves alimentado com bacitracina. Aos 42 dias, não houve efeito dos tratamentos sobre o desempenho. Aves que não receberam nenhum aditivo não apresentaram maior contagem de enterobactérias, sendo semelhantes aos demais tratamentos. A adição do probiótico e do simbiótico não compromete o desempenho dos frangos e não altera a contagem de Enterobacteriaceae.(AU)

Molecular genotyping and epidemiology of Mycobacterium tuberculosis isolates obtained from inmates of correctional institutions of Campinas, Southeast Brazil

The objective of this study was to investigate the possible transmission of tuberculosis among 39 inmates with positive Mycobacterium tuberculosis smears in four correctional institutions located in Campinas City, SP, Brazil over a 19-month period. Fifty-one M. tuberculosis isolates from these inmates were characterized according to the number of IS6110 insertion elements present in their genomic DNA. The number of insertion elements in M. tuberculosis isolates varied from two to twelve. The dendrogram of similarity resulted in the grouping the isolates in six main clusters. These results, associated to epidemiological data, suggested the transmission of tuberculosis among inmates of the same and different institutions inmates. Univariate analysis of epidemiological data (total delay for beginning of treatment, previous treatment, and HIV status) and clustering occurrence showed that only "previous treatment" (OR = 7.65, p = 0.032) was associated with the possible transmission of tuberculosis in the studied prisons.

Comparação de equaçoes de estimativa do consumo máiximo de oxigênio em indivíduos jovens / Comparation of maximal oxygen consumption equations in young people

PURPOSE: To compare the results of equations for estimate of the indirect maximal oxygen consumption with obtained them in test ergoespirometric in young individuals. METHODS: Fifty-two individuals of the masculine sex were submitted to the test of effort with it analyzes direct of gases in treadmill for the determination of the maximum consumption of oxygen (VO2máx). The progressive protocol was used with load increment to each one minute. The obtained results were compared ace equations of Jackson et al and the one of Mathews et al. For the statistical analysis of the results the test of multiple comparisons corrected by the test of Bonferrone was applied. The significance level was of p < 0.05. RESULTS: The average of age was of (21.06 +/- 2.75) years; body mass (70.85 +/- 9.15); thin mass (62.69 +/- 6.86); fat mass (8.30 +/- 4.77); percentile of fat (11.34 +/- 5.45) and maximun heartrate of (191.73 +/- 7.84). The equations of Jackson et al (VO2máx = 49.29 +/- 2.95) for a standard mistake of (EPE = 0.41) and Mathews et al (VO2máx = 37.43 +/-. 2.14) with a (EPE = 0,31) presenting tendency in underestimating the consumption of oxygen for the sample that was of (VO2máx = 55.34 +/- 8.34) for a (EPE = 1.16), being observed significant difference in relation to measure of the VO2máx obtained in test of effort (p < 0.05), for the value of Wilks'E = 0.044; F (2.500) = 539.27; p = 0.001. CONCLUSION: The equations didn't present a power of reliable estimate for the studied population.