RESUMEN
Tuftsin, a naturally occurring tetrapeptide with a sequence Thr-Lys-Pro-Arg was evaluated for its in vivo protective effect against cyclophosphamide-induced genotoxicity and oxidative stress in Swiss albino mice. The anticancer drug cyclophosphamide (CP) was administered intra-peritonially to induce mutagenic effect. The drug treatment caused significant increase in chromosomal aberrations, formation of micronucleated polychromatic erythrocytes (MNPCE's), as well as oxidative stress and decrease in lipid peroxidation in liver of the animals. The pretreatment with tuftsin abolished such effects in dose-dependent manner and also increased mitotic index in the experimental animals. Results of the present study validated chemo-preventive properties of tuftsin against CP-induced chromosomal mutations and cellular injury of liver by oxidative stress.
Asunto(s)
Animales , Antioxidantes/administración & dosificación , Antioxidantes/farmacología , Aberraciones Cromosómicas/inducido químicamente , Aberraciones Cromosómicas/efectos de los fármacos , Ciclofosfamida , Daño del ADN/efectos de los fármacos , Relación Dosis-Respuesta a Droga , Eritrocitos/efectos de los fármacos , Eritrocitos/patología , Femenino , Peroxidación de Lípido/efectos de los fármacos , Liposomas , Hígado/efectos de los fármacos , Hígado/fisiopatología , Ratones , Pruebas de Micronúcleos , Mitosis/efectos de los fármacos , Estrés Oxidativo/efectos de los fármacos , Distribución Aleatoria , Tuftsina/administración & dosificaciónRESUMEN
The frequency of expression of immunoglobulin (Ig) light and heavy chain isotypes was analyzed in myeloma proteins (M-proteins) from sera of 40 Indian patients with clinically established multiple myeloma. Patients samples were screened by a combination of electrophoresis, immunoelectrophoresis (IEP) and ELISA techniques in this study. We found that majority of the myeloma proteins (58%) were of the IgG isotype followed by IgA (24%) and biclonal gammopathy associated with IgG and IgA (5%). Both kappa and lambda light chains were associated with the heavy chain isotypes. We recommend the triangular combination for detection of M-proteins and biclonal gammopathy of cancerous plasma cells as biomarkers for diagnosis of myeloma.
Asunto(s)
Ensayo de Inmunoadsorción Enzimática , Humanos , Inmunoelectroforesis , Cadenas Pesadas de Inmunoglobulina/sangre , Cadenas Ligeras de Inmunoglobulina/sangre , Inmunoglobulinas/sangre , Mieloma Múltiple/inmunología , PronósticoRESUMEN
The possibility of using specific polyclonal antibodies for effective site specific drug targeting to malaria infected erythrocytes has been examined. For this purpose, rabbit polyclonal antiserum was raised against Plasmodium berghei infected mouse erythrocytes (IRBC) and extensively absorbed with normal erythrocytes (NRBC). Absorbed antiserum specifically recognized IRBC. F(ab')2-fragments of these antibodies were coupled to chloroquine (chq) laden liposomes. These immunoliposomes when tested in vivo significantly suppressed malarial infection in mice.