RESUMEN
Nucleotide sequence and phylogenetic analysis of the VP1 structural protein have been used extensively as diagnostic and epidemiological tools for foot and mouth disease virus (FMDV). In this report we have applied this methodology to the analysis of the VP1 coding sequence from FMDV strains isolated in Argentina during 1993-1994. The results demonstrated that the field isolates were related to the vaccine strains used at that time. However the involvement of the vaccine virus appeared to be different for outbreaks caused by FMD viruses type O or C. These data provide a database essential for determining the origin of new epizootics.
Asunto(s)
Animales , Bovinos , Aphthovirus , Enfermedades de los Bovinos/virología , Fiebre Aftosa , Antígenos Virales/genética , Antígenos Virales/inmunología , Aphthovirus , Argentina , Secuencia de Bases , Proteínas de la Cápside , Brotes de Enfermedades , Enfermedades de los Bovinos/epidemiología , Enfermedades de los Bovinos/transmisión , Fiebre Aftosa , Datos de Secuencia Molecular , Filogenia , Estudios Retrospectivos , ARN Viral , Alineación de Secuencia , Homología de Secuencia de Ácido Nucleico , Serotipificación , Vacunas ViralesRESUMEN
The BVDV glycoproteins gp48 and gp53 were expressed in the baculovirus eukaryotic system. Both recombinant proteins were recognized in western blot analysis by monoclonal antibodies and polyclonal serum. Immunofluorescent test demonstrated that gp53 was localized on the cell surface whereas gp48 was in the cytoplasm. The expressed proteins were extracted by non-denaturing detergent treatment. Rabbit antiserum raised against gp53 recombinant protein efficiently neutralized the virus. These results demonstrate that the recombinant proteins have immunological properties similar to those of the native viral protein and that they can be useful as diagnostic reagents.