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Braz. j. med. biol. res ; 27(3): 623-6, Mar. 1994. ilus
Artículo en Inglés | LILACS | ID: lil-148934

RESUMEN

The 18-kDa protein from Mycobacterium leprae is a major target for the immune response in leprosy. We have developed a system to express this antigen in yeast as a fusion protein with the C-terminal region of the yeast membrane protein GAS1, which would render the recombinant protein anchored to the plasma membrane by a glycosylphosphatidylinositol (GPI) anchor. Cells lacking the GAS1 gene and transformed with the hybrid 18-kDa-GAS1 construct express a polypeptide that reacts with an 18-kDa-specific monoclonal antibody. In addition, these cells react with an alpha-CRD antibody after GPI-PLC treatment. The non-transformed cells are negative. These data indicate that our system may be suitable for the expression of foreign proteins in yeast in a GPI-anchored form


Asunto(s)
Glicosilfosfatidilinositoles/genética , Mycobacterium leprae/inmunología , Proteínas Bacterianas/genética , Genes Fúngicos , Vectores Genéticos , Glicosilfosfatidilinositoles/inmunología , Glicoproteínas de Membrana/genética , Glicoproteínas de Membrana/inmunología , Mycobacterium leprae/genética , Proteínas Bacterianas/inmunología , Proteínas Fúngicas/genética , Saccharomyces cerevisiae/genética , Saccharomyces cerevisiae/inmunología
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