RESUMEN
Objective: Multiple Myeloma [MM] is a heterogeneous cytogenetic disorder in which clonal plasma cells proliferate in the bone marrow [BM] and cause bone destruction. The BM microenvironment plays a crucial role in pathogenesis of this disease, and mesenchymal stem cells [MSCs] are one of the key players. Herein, we propose to investigate the expressions of hsa-MIR-204, runt-related transcription factor 2 [RUNX2], peroxisome proliferator-activated receptor gamma [PPAR gamma], and B-cell lymphoma 2 [BCL2] as factors involved in osteogenesis, adipogenesis, and MSC survival in BM-MSCs from MM patients and normal individuals
Materials and Methods: In this experimental study, we isolated MSCs from BM aspirates of MM patients and healthy donors. Total RNA were extracted before and after co-culture with L363 myeloma cells. Gene expressions of RUNX2, PPAR gamma, BCL2, and hsa-MIR-204 were assessed by quantitive real time polymerase chain reaction [qRT-PCR]
Results: Higher levels of RUNX2, PPAR gamma, and hsa-MIR-204 expressions existed in MM-MSCs compared to normally derived [ND]-MSCs. BCL2 expression decreased in MM-MSCs. We observed different results in the co-culture model
Conclusion: In general, the MM-MSCs gene expression profile differed compared to ND-MSCs. Upregulation of RUNX2, PPAR gamma, and hsa-MIR-204 in MM-MSCs compared to ND-MSCs would result in formation of bone defects. Downregulation of BCL2 would lead to MM-MSC cell death