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Objective:To study the function and possible mechanism of Fufang-Zhongjiefeng aerosol in the treatment of chronic pharyngitis. Method:Sixty SD rats were randomly divided into normal group, model group, low-, medium-, and high-dose Fufang-Zhongjiefeng aerosol group, and positive drug group, with 10 rats in each group. Except the normal group, the pharynx of other groups were injected with group A beta-hemolytic streptococcus (GAS) to establish chronic pharyngitis rat model. After the modeling, the low-, medium-, and high-dose Fufang-Zhongjiefeng aerosol groups were sonicated with 20 ml of 0.9% normal saline to dissolve the Fufang-Zhongjiefeng aerosol 2.33, 4.66, 9.32 g/kg, respectively. The normal group and the model group were given the same volume of normal saline, and the positive drug group was intraperitoneally injected with ampicillin sodium 0.93 g/kg once a day for 14 days. The pathological changes of pharyngeal mucosa were detected by hematoxylin and eosin (HE) staining. The levels of tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β) and nterleukin-6 (IL-6) in serum were detected by Enzyme-linked Immunosorbent Assay (ELISA). The expression levels of toll-like receptor 4 (TLR4), myeloid differentiation factor 88 (MyD88) and nuclear factor kappa B (NF-κB) p65 protein in pharyngeal mucosa were detected by immunohistochemistry. The expression levels of TLR4, MyD88 and NF-κB p65 mRNA in pharyngeal mucosa were detected by quantitative real-time reverse transcription-polymerase chain reaction (RT-qPCR). Results:Compared with the model group, the serum TNF-α, IL-1β and IL-6 levels of rats in the Fufang-Zhongjiefeng aerosol low-, medium-, high-dose group and the positive drug group were significantly reduced ( P<0.05). The expression of TLR-4, MyD88 and NF-κB p65 protein in the pharyngeal mucosal tissue of rats in the low-, medium-, high-dose Fufang-Zhongjiefeng aerosol group and the positive drug group were significantly reduced ( P<0.05). The expressions of TLR-4 mRNA (1.17 ± 0.41, 2.44 ± 1.06, 1.25 ± 0.34 vs. 3.87 ± 1.43), MyD88 mRNA (1.15 ± 0.53, 1.75 ± 0.36, 1.09 ± 0.14 vs. 2.44 ± 0.19), and NF-κB p65 mRNA (1.97 ± 0.51, 2.64 ± 0.26, 2.31 ± 0.44 vs. 5.08 ± 0.34) in the pharyngeal mucosa tissue of rats in the medium-, high-dose group and the positive drug group were significantly reduced ( P<0.05). Conclusion:Fufang-Zhongjiefeng aerosol can effectively treat chronic pharyngitis, which could inhibit the expression of TLR-4, MyD88, and NF-κB to reduce the expression of inflammatory factors so as to play the rold of anti-inflammatory effect.
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Objective To research the capacity for health emergency of the county-level in Chongqing .Methods CDCs ,adminis-trative departments of public health ,health supervision departments ,hospitals in all county of Chongqing were surveyed by ques-tionnaires .Results 84 .62% of the county governments had involved health emergency system construction into the national eco-nomic and social development planning ;76 .92% of the county government have funded for health emergency daily work ;58 .97% of the counties have set by compiling department of health emergency office .All districts and counties formed the health emergency re-sponse team .79 .49% of the districts and counties established health emergency stockpile management system .94 .87% of the dis-tricts and counties have carried out the propaganda work of health emergency .89 .74% of the CDCs periodically analysed for infec-tious diseases and public health emergencies .89 .74% (35/39) of the districts and counties were issued the corresponding level of the public emergency medical rescue emergency plan .All districts and counties carried out the health emergency disposal in a time-ly .69 .23% of the districts and counties brought the health emergency disposal into the pathogenesis and residual on business ,such as death subsidies and pension system .Conclusion T he health emergency system in Chongqing has been basically established ,but there are still many difficulties and problems needing to solve .
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ABSTRACT:In order to explore the spatial clustering and influential factors of HFMD in Chongqing ,China from 2008 to 2012 ,spatial autocorrelation and spatial regression analysis (using the spatial lag model in this study ) were carried out using the HFMD data of 38 districts (counties) from 2008-2012 in Chongqing by OpenGeoDa ,and the HFMD case‐based data was collected from the Disease Supervision Information Management System of Chongqing Center for Disease Control and Preven‐tion .We found that the global Moran’s I coefficient of Chongqing from 2009 to 2012 was 0 .458 7 ,0 .567 5 ,0 .398 6 ,and 0 .606 0(P0 .05) .Results of multi‐factor spatial lag regression analysis demonstra‐ted that the incidence of HFMD was positively related with urban rate (β=1 .667 6 , P=0 .001 6) ,and negatively correlated with medical technical personnel per thousand (β= -0 .000 2 ,P=0 .019 8) .In general ,the incidence of HFMD was found ge‐ographically clustered in Chongqing from 2009 to 2012 which was significantly influenced by urban rate and medical technical personnel per thousand population ,and while the urban rate was the main factor .
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Objective To work out the allocation scheme of the mobile hospital's major equipments based on the demand for the health emergency handling task of Chongqing Municipality so as to provide the basis for the construction proj ects of the Chongqing's mobile hospital.Methods The focus group discussion method was used to draft the alternative scheme;the Delphi method was applied to perform the investigation on the alternative scheme and according to the results of the experts consultation,the allocation scheme of major equipments in the Chongqing's mobile hospital was worked out.Results 20 items such as the operation vehicle,X-ray vehicle and inflatable tents were screened out as the mobile hospital's major equipments for constituting the framework of the main equipments for the Chongqing's mobile hospital.Conclusion The allocation scheme research provides the reference method and standard for constructing the mobile hospitals by the local health department.The mobile hospitals constructed according to this scheme by Chongqing Municipality played an important role in Lushan earthquake medical rescue in 2013.
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Objective To explore the feasibility of using the prospective space-time analysis and early warning in health emer-gency command system in Chongqing .Methods Prospective space-time scan analysis method was used for the early detection of disease outbreaks in mumps patients from 5 districts(Jiangbei ,Shapingba ,Dadukou ,Jiulongpo ,Nan′an ,) in Chongqing in 2006 ,then verified the aggregation using the actual reported mumps outbreaks .Results The results showed that 3 early warning signals at October 9th ,November 13th ,December 4th ,involving a total of 5 locations(streets/towns) .The strongest warning happened on Oc-tober 9th in Zengjia town of Shapingba district ,and Jingfeng town of Jiulongpo district ,the recurrence interval was 1 916 495 550 992 471 years .There were 2 mumps outbreaks have been reported in the surveyed areas .Both the the 2 outbreaks were detected by the prospective space-time scan statistic 5-6 days early than the actually occurred time .Conclusion The prospective space-time scan analysis method could be applied to the disease monitoring and early warning in health emergency command decision-making system in Chongqing .