Protective effect of Zuroor-e-Qula' against gastric mucosal damage: an ultrastructural study

The gastro-protective activity of the PHF [Zuroor-e-qula'] and its extracts was assessed in three different models of experimentally induced gastric ulcers in rats. In case of both preventive and curative treatments at 400 mg/kg dose the PHF and the aqueous and alcoholic extracts reduced gastric lesions significantly which were induced by HCl/ethanol. Significant changes in gastric parameters such as pH, volume of gastric juice and gastric acidity after oral administration of the samples under ligated pylorus [Shay] conditions. Also, in this paper, we report the ultrastructural observations of gastric mucosa. The ultrastructural changes were observed by Scanning Electron Microscopy [SEM]. The preventive as well as curative treatments in rats revealed a protective action against HCl/ethanol induced gastric mucosal damage. Probably, polyphenols, flavonoids, essential and fixed oils of the formulation were involved

Content uniformity study of polyherbal formulation by different analytical techniques, an attempt

The alcoholic extract of lie Polyherbal Formulation [PHF] shown 7 spots in solvent phase toluene: ethyl acetate: ethanol [3:3:4] upon treatment with 5- 10% ethanoIic Sulphuric acid followed by heating at 105°C for 45 mins. These seven spots were correlated with the co-chromatography of isolated marker compounds. The chromatogram properties were as follows: Compound I [PHF, 7 spots at Rf 0.12, 0.15. 0.24. 0.33. 0. 51, 0 66 and 0.83]; compound 2 [0.12. darkbrown]. compound 3 [0.15, pink], compound 4 [0.24. light brown]. compound 5 [0.33, brown], compound 6 [0.5 light red to brown], compound 7 [0.66. light red to while], and compound 8 [0.83, brownish blue]. The fixed oil was isolated from the formulation by solvent extraction method [petroleum ether] and the volatile oil was isolated by hydro- distillation method and was analyzed by GLC and GC-MS studies

Anti-inflammatory and anti-microbial activity of zuroor-e-gaozaban a polyherbal unani formulation

Zuroor-e-gaozaban is a polyherbal Unani formulation, traditionally used as a local application for treating oral white stomatitis/white thrush and traditionally known to be having antiseptic action. The formulation was screened for anti-inflammatory activity and in vitro antimicrobial activity. Aqueous suspension of the formulation was screened for in vivo anti-inflammatory activity, at dose levels of 100, 200, 400 mg/kg, using the carrageenan induced rat paw oedema test. The formulation showed a very mild anti-inflammatory activity; the results were compared with standard anti-inflammatory drug, indomethacin 20 mg/kg orally. The extracts and suspensions prepared from the formulation were screened for antimicrobial activity using agar well diffusion method, against standard strains of two bacteria, Staphylococcus aureus and E. coli, and a strain of fungus Candida albicans. The extracts and suspensions were not found effective against both the standard bacterial strains and the strain of fungus. The results were compared with the results obtained using standard antibiotics, vancomycin, and amikacin discs

Anti-inflammatory and antimicrobial activity of sufoof-e-satt-E-Gilo-sartani a polyherbal unani formulation

Sufoof-e-san-e-gilo-sartani, is a polyherbal Unani formulation, traditionally used for treating phthisis, tuberculosis, bronchitis, diarrhoea, traditionally known to have antipyretic, cough relieving and soothing actions. The formulation was screened for anti-inflammatory activity and in vitro antibacterial activity. Aqueous suspension of the formulation was screened for in vivo anti-inflammatory activity at dose levels of 100, 200 and 400 mg/kg, using carrageenan induced rat paw oedema test. The formulation showed a moderate anti-inflammatory activity, the results were compared with standard anti-inflammatory drug, Indomethacin @ 20 mg/kg orally. Various extracts and suspensions prepared from the formulation were screened for antibacterial activity using agar well diffusion method against two standard strains of bacteria, viz. Staphylococcus aureus and Escherichia coli. However, the extracts and suspensions were not found effective against both the standard bacterial strains. The results were compared with the results obtained using standard antibiotics, vancomycin and amikacin discs

Pharmacological evaluation of Zuroor-e-Qula. : a Polyherbal Formulation

The current work was undertaken to standardize the route of administration and the anti-inflammatory activity of a polyherbal formulation. The anti-inflammatory activity of Zuroor-e-Qula was evaluated on carrageenan and histamine induced rat hind paw edema models, upon oral administration and topical application. The formulation at a dose of 400mg/kg was found to possess significant anti-inflammatory activity on the tested experimental models. The formulation exhibited maximum anti-inflammatory effect, that is 82.58 and 83.57% at the end of 24 h with carrageenan and histamine induced rat paw edema, respectively upon oral administration. But the effect was negligible, that is 32.78% at the end of 6 h with carrageenan induced rat paw edema, upon local application. In a chronic test, the formulation [400 mg/kg] showed 32.29% reduction in granuloma weight. The histopathological studies of the granuloma tissue were carried out. The effect produced by the formulation, upon oral administration, was comparable to that of Diclofenac sodium, a prototype of a non-steroidal anti-inflammatory agent

Standrdization of Zuroor-e-Qula. A polyherbal formulation

Zuroor-e-Qula, one of the traditional polyherbal formulations is used as anti-microbial and anti-inflammatory drug. It contains mixtures of various powdered ingredients like kathsafaid [Acacia leucophloea], tabasheer [Bambusa bambos], kafoor [Cinnamomum camphora], dana heel khurd [Elettaria cardamomum], gulnarfarsi [Punica granatum], gul-e-surkh [Rosa damascena], kabab-e-khandan [Zanthoxylum armatum]. The present study involves the pharmacognostical and phytochemical standardization of this polyherbal formulation. For microscopic, diagnostic characters like epidermis of testa, endospermic cells, characteristic long fibres and other specific features were observed in the formulation. Physicochemical characters like ash values, extractive values and loss on drying were also evaluated. The preliminary phytochemical investigation revealed the presence of tannins, fixed oils, volatile oils, phenolic compounds and flavonoids. Alcoholic, petroleum ether, chloroform and aqueous extracts of the formulation were subjected to thin layer chromatographic analysis in different solvent systems and the presence of four components with different R[f] values were observed. The fluorescence analysis was also studied. Phytochemical assay for different constituents was also done