RESUMEN
Aim: The aim of this study was to find the relationship between rs1799964 in TNF-alpha gene as well as rs1051208 of RAF1 gene SNPs on GC in an Iranian population
Background: Gastric cancer [GC] is the second leading cause of cancer-related death worldwide after lung cancer. Tumor necrosis factor [TNF] is one of the most important factors in the pathogenesis of this cancer. Single nucleotide polymorphisms have a principle role in gene expression of TNF-alpha and miRNAs which may lead to gastric cancer
Methods: In a case-control study, we investigated the risk of GC in 198 Iranians. For this purpose, 5 mL of peripheral blood was collected in EDTA -containing tube and genomic DNA was isolated. Genotyping of SNPs was also performed by PCR-RFLP; to approve the outcome, 10% of genotyping results with RFLP were sequenced
Results: The comparison between case and control groups revealed a significant association between the rs1051208 C allele of RAF1 gene and GC [P = 0.04]. We did not observe any remarkable association between TNF-alpha -1031 in gastric cancer patients and the healthy control group
Conclusion: The results indicated that C allele in RAF1 gene plays a role in susceptibility to gastric cancer. Therefore, SNPs are among notable biomarkers for predicting susceptibility to dreadful diseases, especially cancers
RESUMEN
Background: It has been shown by recent studies that there is a significant association between genetic polymorphisms near the regulatory of IL28B gene and response to treatment in viral diseases such as hepatitis C. However, genetic factors involving in infection progression to chronic diseases have not been determined yet. In this study, the association of these IL28B polymorphisms with susceptibility to chronic hepatitis C virus infection has been analyzed
Materials and methods: In this case- control study, 110 patients infected with chronic hepatitis C and 110 healthy individuals were studied. The fragments covering rs8099917 and rs12979860 were amplified by the polymerase chain reaction [PCR] method and genotyped by restriction fragment length polymorphism digestion [RFLP] method using NmuCI and BstUIendonuleases enzymes
Results: Results showed that rs12979860CC genotype were the most frequent which followed by CT and TT. However, rs8099917TT was the dominant genotype. Allele frequencies were included: Rs8099917 T=72.3%, G=27.7% and rs12979860 C=69.5%, T=30.5% at patients and rs8099917 T=80%, G=20% and rs12979860 C=73.2%, T=26.8% at control group
Conclusion: The Results showed that T allele is more prevalent than G at rs12979860 polymorphism and the frequency of C allele is higher than T at rs8099917 position. However, rs809917TT and rs12979860 CC genotypes are the most common genotypes among patients population with chronic hepatitis C