Biological features and ultrastructure of human umbilical cord mesenchymal stem cells / 中国医学科学院学报

<p><b>OBJECTIVE</b>To isolate and culture human umbilical cord mesenchymal stem cells (MSCs) and explore their biological features and ultrastructure.</p><p><b>METHODS</b>After isolating MSCs from the human umbilical cord, the proliferation, cycle, and apoptosis were observed. The cell ultrastructure was observed under transmission electron microscope. The cytokines including vascular endothelial growth factor (VEGF), hepatocyte growth factor (HGF), and insulin-like growth factor-1 (IGF-1) were detected using enzyme-linked immunosorbent assay.</p><p><b>RESULTS</b>Human umbilical cord MSCs had fibroblast-like morphology and increased proliferation capability. Ultrastructural analysis showed that the MSCs had active cellular metabolism and strong migration and differentiation capabilities. Meanwhile, they could secrete anti-apoptotic cytokines such as VEGF, IGF-1, and HGF.</p><p><b>CONCLUSION</b>Human umbilical cord MSCs can secrete many anti-apoptotic cytokine and have good biological features.</p>

Comparative genomic hybridization analysis of nonsyndromic cleft lip with palate / 中华整形外科杂志

<p><b>OBJECTIVE</b>To identify the genetic alterations in nonsyndromic cleft lip and palate (NSCLP).</p><p><b>METHODS</b>Comparative genomic hybridization was applied to investigate the genomic imbalance (the gain or loss of genetic material) in 7 cases of NSCLP.</p><p><b>RESULTS</b>It showed that the loss of chromosome DNA copies happened in chromosome 6, 7, 10, 13, 14, 16, 20, 22 and the gain of chromosome DNA copies happened in chromosome 5, 15, 18, 19. Conclusions 13q had a high frequency (71.4%) of chromosome loss.</p><p><b>CONCLUSIONS</b>Abnormal chromosome DNA copies happen in all the patients with NSCLP. Most of the patients have chromosome DNA copies loss. It suggests that loss of inhibitory gene may be related to the NSCLP. The related inhibitory gene may locate in 13q.</p>