A histological study of three-dimensional external zygomatic suture distraction osteogenesis / 中华口腔医学杂志

<p><b>OBJECTIVE</b>To explore the histological change in suture of zygomatic bone for the zygomatic suture direct distraction osteogenesis.</p><p><b>METHODS</b>The zygomatic bone was distracted by 3-D external distraction appliance without osteotomy. The specimens were taken 1, 3, 5 and 8 weeks after, and then examined histologically and compared with the blank contralateral side.</p><p><b>RESULTS</b>There were lots of fibroblasts, osteoblasts and capillary vessels in the distracted suture tissues one week after distraction, and the fibers were observed to connect the sides of suture and arranged orderly. The surfaces of the expanded suture were irregular. Bone formation was active in the expanded side. The bone trabeculae were mature and oriented in the direction of distraction in the distracted sides at 3 weeks. A great amount of new woven bones were found in 5-week specimen. New bones were formed completely 8 weeks after the distraction.</p><p><b>CONCLUSIONS</b>New bone formed rapidly in the distracted side of zygomatic bone under suture distraction osteogenesis without osteotomy.</p>

Effects of aminoguanidine on the expression of NOS in facial nerve and surrounding tissues of traumatic facial paralysis rats / 华西口腔医学杂志

<p><b>OBJECTIVE</b>To study the effects of inducible NOS inhibitor aminoguanidine on the expression of NOS in facial nerve and surrounding tissue of traumatic facial paralysis rats.</p><p><b>METHODS</b>A small dose of aminoguanidine were intraperitoneally injected into rats before and after facial paralysis. The facial nerve and surrounding tissues were cut at different time point. Immunohistochemical ABC method was used to study the changes of NOS expression in facial nerve and surrounding tissues.</p><p><b>RESULTS</b>The inducible NOS immunoreactivity was obvious inhibited in the facial nerve and surrounding tissues in aminoguanidine group.</p><p><b>CONCLUSION</b>Aminoguanidine chronic treatment can obvious inhibit the inducible NOS expression in the facial nerve and surrounding tissues. Aminoguanidine can improve the regeneration of facial nerve and the recovery of traumatic tissues.</p>

Reconstruction correction to Reconstrction of mandibular bone defects by using the techniques correction to teachniques of the reverse engineering and rapid prototyping / 中华口腔医学杂志

<p><b>OBJECTIVE</b>To evaluate the feasibility of designing and fabricating customized titanium bone substitutes to restore mandibular bone defects using reverse engineering (RE) and rapid prototyping (RP) techniques.</p><p><b>METHODS</b>Titanium tray for mandibular defects were designed and fabricated through multi-step procedures of reverse engineering and rapid prototyping, then in operation it was filled with cancellous bone and fixed.</p><p><b>RESULTS</b>The bone substitutes fabricated by this method had been successfully put into clinical use for maxillofacial surgery in 2 patients and got a satisfactory result.</p><p><b>CONCLUSIONS</b>Reverse engineering combining with rapid prototyping could accomplish the design and manufacture of implant for the restoration of mandibular bone defects.</p>

Effect of recombinant human basic fibroblast growth factor on angiogenesis during mandible fracture healing in rabbits / 中华创伤杂志(英文版)

<p><b>OBJECTIVE</b>To investigate the effect of recombinant human basic fibroblast growth factor (rhbFGF) on angiogenesis during mandible fracture healing in rabbit.</p><p><b>METHODS</b>Fifty adult white rabbits were used for animal model and randomly divided into a control group (25 rabbits) and an experimental group (25 rabbits). The membranous complex of rhbFGF and bovine type I collagen was prepared and implanted into the rabbit mandible fracture site under periosteum. The animals were sacrificed on 7, 14, 28, 56 and 84 days respectively after operation and the whole mandibles were harvested. The expression of factor VIII related antigen (F8-RA) in callus was examined with immunohistochemical staining.</p><p><b>RESULTS</b>The amounts of microvascular formation in calluses in the rhbFGF-treating group on days 7, 14, 28 and 56 were more than those of the control group (P<0.01).</p><p><b>CONCLUSIONS</b>The results indicated that rhbFGF could stimulate microvascular formation during mandible fracture healing in rabbits.</p>

An experimental study of 3-dimension zygomatic suture extension for suture osteogenesis / 中华整形外科杂志

<p><b>OBJECTIVE</b>To explore a methods of 3-Dimension expansion of zygomatic suture in a goat model.</p><p><b>METHODS</b>Seven goats were used in this study. The 3-D extensive applicator was designed and used to extend the zygomatic suture of the goats by placing it in the zygomatic bone through an infraorbital incision. Ten days after the first operation, it was gradually extended on a speed of 0.09 cm/d for 7 days. The zygomatic movement and the osteogenesis of the suture was evaluated in two weeks.</p><p><b>RESULTS</b>The zygomatic bone was extended for 0.6 cm long in average, and the osteogenesis was also shown significantly in the suture.</p><p><b>CONCLUSION</b>The above mentioned technique could be a safe and effect method to be applied for the zygomatic extension.</p>

Effects of L-nitroarginine on the recovery of traumatic facial paralysis / 中华口腔医学杂志

<p><b>OBJECTIVE</b>To study the effects of constitutive nitric oxide synthase inhibitor L-nitroarginine on the recovery of traumatic facial paralysis in rats and the changes of the expression of cNOS and OX42 in the facial nucleus.</p><p><b>METHODS</b>L-nitroarginine was intraperitoneally injected into rats and the recovery of facial paralysis was observed at different time point. and the changes of cNOS and OX42 positive neurons were studied in facial nucleus.</p><p><b>RESULTS</b>Treatment of L-nitroarginine could remarkably inhibit the recovery of traumatic facial paralysis. The cNOS immunoactivity was obvious inhibited in facial nucleus, while the OX42 immunoactivity was obvious increased.</p><p><b>CONCLUSION</b>Endogenous nitric oxide may play an important mediator role on the recovery of traumatic facial paralysis.</p>