Isolation and identification of cytotoxic compounds from a fruticose lichen Roccella montagnei, and it's in silico docking study against CDK-10

ABSTRACT Roccella montagnei Bél. belongs to lichen family Roccelleceae growing luxuriantly along the coastal regions of India. As Roccella has been shown to be bioactive, we prepared methanolic extract and assessed its anticancer potential. The methanolic extract showed significant in vitro cytotoxic activity against four human cancer cell lines such as colon (DLD-1, SW-620), breast (MCF-7), head and neck (FaDu). This prompted us to isolate bioactive compounds through column chromatography. Two compounds roccellic acid and everninic acid have been isolated, out of which everninic acid is reported for the first time. Both the compounds have been tested for in vitro cytotoxic activity in which roccellic acid showed strong anticancer activity as compared to the everninic acid. Cyclin Dependent Kinase (CDK-10) contributes to proliferation of cancer cells, and aberrant activity of these kinases has been reported in a wide variety of human cancers. These kinases therefore constitute biomarkers of proliferation and attractive pharmacological targets for development of anticancer therapeutics. Therefore both the isolated compounds were tested for in silico molecular docking study against Cyclin Dependent Kinase isomer enzyme to support the cytotoxic activity.

Endodontic and Esthetic Management of Hypolplastic Turner’s Teeth with Severely Dilacerated Roots.

Dilaceration refer to an angulation or sharp bend or curve in the root or crown of formed tooth. It is usually occur due to trauma that lead to white or yellow brown discoloration, crown dilaceration, root dilacerations, crown duplication, root duplication, odontoma like malformation etc. This paper elaborated a useful clinical considerations for diagnosis and treatment planning of a severely dilacerated root of malformed tooth having turner’s hypoplasia.

Biological Post: A Reconstructive Approach to Achieve Aesthetics.

Traumatic injuries lead to fracture of anterior teeth, frequently occurs in dentistry and the prevalence rate of fractured anterior teeth, as a result of traumatic injuries, occurs 8.1 in 1000. The fracture of a tooth is itself a traumatic incident for young patients which leads to functional, aesthetic, psychological problems and reduces patient's quality of life. Various materials are available to restore these types of traumatic injuries, but till date there is no material that has been proved to be as effective as natural structure considering mechanical and biological properties. This case report represents the management of traumatized fractured mandibular anterior teeth with biological posts and coronal restorations with composite.