Effect of Xiaoer Fuxie Waifu powder on the ultrastructure of intestinal cajal cells and expression of neurotransmitter receptor VIP-R1 in rats with diarrhea / 解放军医学杂志

Objective To investigate the effect of Xiaoer Fuxie Waifu powder on intestinal Cajal cells (ICC) and the expression of vasoactive peptide receptor 1 (VIP-R1) in rats with diarrhea. Methods Thirty Wistar rats were divided randomly into three groups: control group, model group, and treatment group (10 each). Folium sennae (2ml/100g) was gavaged to reproduce the diarrheal model. Xiaoer Fuxie Waifu powder was applied topically in the treatment group. Transmission electron microscope was used to observe the changes in ultrastructure of ICC after application of the drug. Immunohistochemistry was used to observe the distribution of VIP-R1 in the intestine of diarrheic rats. RT-PCR and Western blotting were used to determine the expressions of mRNA and protein of VIP-R1. Results The ultrastructure of ICC showed that the drug treatment could normalize the cellular morphology and gap junction. VIP-R1 was found to be distributed mainly between circular muscle and longitudinal muscle, in the myenteric nerve plexus of the small intestine, and was found around the epithelial cells in the mucosal layer of the colon and lamina propria cells. Xiaoer Fuxie Waifu powder inhibited the secretion of VIP-R1 and down-regulated the mRNA and protein expression of VIP-R1. Conclusion Xiaoer Fuxie Waifu powder can repair the injured ICC of diarrheic rats and cure diarrhea by down-regulating the expression of VIP-R1.

Value of radiographic esophageal imaging in determining an optimal atrial septal puncture site for percutaneous balloon mitral valvuloplasty

To investigate the value of radiographic esophageal imaging in facilitating transseptal catheterization in patients undergoing percutaneous balloon mitral valvuloplasty. A total of 468 patients were randomized into either the study group [n = 234], in which radiographic esophageal imaging by the oral administration of a contrast media took place, or the control group [n = 234], in which the Ross technique was used. Of the 468 patients, 203 were males and 265 were females. The average ages of the study and control groups were 53 +/- 16 and 51 +/- 17 years, respectively. The patients had severe left atrial enlargement as measured using 2-dimensional echocardiography. In the study group, the left atrial impression on the esophagus was clearly seen, and was used to identify the puncture site on the right atrial side for the passage of the transseptal catheter. In the control group, the left atrial silhouette was not clearly shown by fluoroscopy in 112 patients [47.5%]. The success rate of transseptal catheterization in the study group was higher than in the control group [99.6 vs. 45.7%, p = 0.0001]. There were no complications in the study group, but pericardial tamponade occurred in 1 patient in the control group. Radiographic esophageal imaging facilitates the identification of an optimal atrial transseptal puncture site, and improves the success rate of transseptal catheterization in patients undergoing percutaneous balloon mitral valvuloplasty

In vivo/in vitro evaluation of Kangfuxin colon targeting capsules / 中国中药杂志

<p><b>OBJECTIVE</b>To evaluate the release in fixed position of pH-dependent and enzyme-dependent Kangfuxin colon targeting capsules in vivo and in vitro.</p><p><b>METHOD</b>The dissolution was tested in vitro and X-ray radiography was used for the evaluation in vivo.</p><p><b>RESULT</b>After two hours pH-dependent colon targeting in man-made colon fluid, medicine release in fixed position on the whole, colon loc-release. Add enzyme into man-made colon, when enzyme-dependent colon targeting in it, then medicine release quickly, mainly release in fixed position; The conveying time in vivo of pH-dependent and enzyme-dependent capsules have big individuality difference. In the experiment, disintegration is stabilize among individuales, between 2.0-3.5 hours.</p><p><b>CONCLUSION</b>Kangfuxin colon targeting capsules of two principles all release in fixed position to achieve the goal.</p>

Preparation of kangfuxin colon targeting micro-pellets / 中国中药杂志

<p><b>OBJECTIVE</b>To prepare coated micro-pellets of pH-dependent and enzyme-dependent kangfuxin colon targeting delivery system, to make them go to colon, then release, educe partial effect.</p><p><b>METHOD</b>We eploy pan-pill to prepare simple pellets, and prepare tunicatus pellets with fluidized bed coating. We investigated the preparation and parameter of pellets, so, we bolting the best shaping and tunicatus artwork.</p><p><b>RESULT</b>The ingredients for preparing the micro-pellets are 125% starch +2% CMC-Na, and add 30% ethanol to be binder, pellets were coated with Eudragit S100 to prepare ph-dependent and pectin-HPMC to prepare enzyme-dependent colon targeting micro-pellets.</p><p><b>CONCLUSION</b>We get two micro-pellets of pH-dependent and enzyme-dependent kangfuxin colon targeting.</p>

Anti-emetic effect of ethanol extract from "Wuzhuyu broth" / 中国中药杂志

<p><b>OBJECTIVE</b>To investigate the anti-emetic effect of ethanol extract from "WuZhuYu broth" and its mechanism.</p><p><b>METHOD</b>Three experiments were carried out test which extract has anti-emetic activity, such as CuSO4-induced pigeon's emetic response, gastric emptying in mice and ACh-induced or 5-HT-induced contraction in vitro gastric muscle in rats. Meanwhile, effect of anti-emetic extract on concentration-response curve to ACh, 5-HT, histamine was investigated.</p><p><b>RESULT</b>50% ethanol extract and 70% ethanol extract were identified as having significantly stronger anti-emetic activities with little side effect, which showed the significant effect on concentration-response curve to ACh, 5-HT, histamine.</p><p><b>CONCLUSION</b>50% ethanol extract and 70% ethanol extract contain more anti-emetic fractions, more anti-emetic fractions can be gained at the concentrations of 50% and 70% ethanol; the mechanism of anti-emetic effect is related to its antagonism to the receptors of ACh, 5-HT, histamine.</p>