RESUMEN
Background: Burn is one of the factors in the spread of disease. To treat burns, several topical medications are required. This study was performed to evaluate the effects of topical nano zinc oxide on skin burns of adult female mice of NMRI
Materials and methods: 30 adult female mice of the NMRI were placed in groups of control1[without burns], control2 [burns without healing], sham [burns distilled water tween 20], experimental 1 [burns and distilled zinc oxide 300mg], and experimental 2 [burns and distilled zinc oxide 500mg]. In sterile conditions and anesthesia, a wound was created with diameter of one centimeter on the back of each mouse. The mice were treated for 21 days and were easy to draw. The thickness of the horny layer, epidermis, dermis, hypodermis, number of hair follicles and number of dermic vessels and vessel diameter, the diameter of the wound and scar was evaluated
Results: Diameter of scar in all groups revealed reduction [P<0.001] compared to control 2. Thickness of horny epiderm significantly increased [p <0.001] in groups of control 1, sham, experimental 1, and 2 compared to control 2. The thickness of the hypoderm increased in groups of sham [p<0.01], control 1, experimental 1, and 2 [P<0.001] compared to control 2. Thickness of the dermis was larger in groups of control 1, sham, experimental 1, and 2 [P <0.001] in comparison of control 2 group. The number of hair follicles was decreased in control 1 group [P<0.01] and increased in groups of sham [p<0.01], and experimental 1 and 2 [P <0.001] compared to control 2. There were no significant differences in the number of dermic vessels and the diameter of dermic vessels between groups
Conclusion: Results showed that nano zinc oxide had good effects on burned skin layers and hair follicles
RESUMEN
Studies have shown that any disruption in wnt signaling pathway is associated with Alzheimer disease [AD]. One of the important molecules involved in activation or inactivation of this pathway is GSK3beta [glycogen syntase kinase3beta]. The main goal of this study was to evaluate GSK3beta phosphorylation by treatment of cells with dihydroepiandrosterone [DHEA], a kind of neurosteroid that decreases in the brain with aging. In this experimental study, neural progenitor cells were obtained from mouse embryos brain. Then, these cells were treated with 1microm concentration of DHEA for 48h. After 48h, the phosphorylation of GSK3beta was analyzed by immunocytochemistry. DHEA increased phosphorylation of GSK3beta in neural cells treated by DHEA, whole in control group, we could not detect the expression of GSK3beta. DHEA can increase phosphorylation of GSK3beta in neural cells and inactivation of GSK3beta can help to cure AD
RESUMEN
Due to increasing clinical demand for adipose tissue, a suitable cell for reconstructive adipose tissue constructs is needed. In this study, we investigated the ability of Human Endometrial-derived stem cells [EnSCs] as a new source of mesenchymal stem cells to differentiate into adipocytes. EnSCs are the abundant and easy available source with no immunological response, for cell replacement therapy. Single-cell suspensions of EnSCs were obtained from endometrial tissues from 10 women experiencing normal menstrual cycles, and were cultured at clonal density [10 cells/cm[2]] or limiting dilution. Endometrial mesenchymal stem cell markers were examined flow cytometry. These cells were treated with adipogenicinducing medium for 28 days. The adipogenic differentiation of the EnSC was assessed by cellular morphology and further confirmed by Oil Red O staining and RTPCR. The BM-MSC differentiated into adipocytes in the presence of adipogenic stimuli for 3 weeks. The flow cytometric analysis showed that the cells were positive for CD90, CD105, CD146 and were negative for CD31, CD34.We showed that the key adipocytes marker PPARa was expressed in mRNA level after 28 days post treatment [PT]. According to our finding, it can be concluded that EnSCs represent a useful in vitro model for human adipogenesis, and provide opportunities to study the stages prior to commitment to the adipocyte lineage