Inhibitory effect of Lactobacillus rhamnosus on pathogenic bacteria isolated from women with bacterial vaginosis

Considering the high prevalence of bacterial vaginosis and its association with urinary tract infection in women and treatment of gynecologic problems occur when a high recurrence of bacterial vaginosis is often treated with antibiotics. The purpose of this study is to investigate the inhibitory effect of Lactobacillus rhamnosus on pathogenic bacteria isolated from women with bacterial vaginosis, respectively. 96 samples from women with bacterial vaginosis discharge referred to health centers dependent Shahid Beheshti University in 91-92 were taken by a gynecologist with a dacron swab and put in sterile tubes containing TSB broth and Thioglycollate broth and were immediately sent to the lab location in cold chain for the next stages of investigation. From Thioglycollate and TSB medium was cultured on blood agar and EMB and Palkam and Differential diagnosis environments, and then incubated for 24 h at 37[degree]C. Strains of Lactobacillus rhamnosus were cultured in MRSA environment and were transfered to the lab. After purification of pathogenic bacteria, MIC methods and antibiogram, Lactobacillus rhamnosus inhibitory effect on pathogenic bacteria is checked. Statistical analysis was done by SPSS software v.16. The results of this study show the inhibitory effect of Lactobacillus rhamnosus on some pathogenic bacteria that cause bacterial vaginosis, including Staphylococcus aureus, Staphylococcus epidermidis, Staphylococcus saprophyticus, Streptococcus agalactiae, Entrococcus, Listeria monocytogenes and E.Coli. Microscopic examination of stained smears of the large number of Lactobacillus and pathogenic bacteria showed reduced. The prevalence of abnormal vaginal discharge, history of drug use means of preventing pregnancy and douching, respectively, 61%, 55%, 42% and 13% respectively. Significant difference was observed between the use and non-use of IUD in women with bacterial vaginosis infection. Our findings indicated the Inhibitory effect of Lactobacillus rhamnosus on the pathogenic bacteria that cause bacterial vaginosis. The results of this study confirm the hypothesis of inhibit of pathogens growth that cause bacterial vaginosis supported by probiotics and can have beneficial effects in the prevention and treatment of bacterial vaginosis

Phenotypical analysis of vibrio cholerae 01 [Eltor and classic] biotypes by scanning electron microscope and transmission electron microscope

Due to repeated cholera outbreaks in Iran and neighboring countries, the present study was performed to determine the prevalence of phenotypes of Ogawa and Inaba serotypes of Vibrio cholera 01 and classic Vibrio cholera 01 biotypes. Materials and methods: Scanning and transmission electron microscopy [SEM and TEM] were applied on 4 species, of Ogawa and Inaba serotypes and two classic serotypes of Vibrio cholera 01. Membrane diameter of Eltor was wider as compared to classic biotype. Number of ribosomes, protein synthesis, length and number of flagella were quite more in comparison with classic biotype. According to our findings, genome of classic biotype is more compact. Keywords: Vibrio cholera, Ogawa serotype, Inaba serotype, Transmission Electron Microscopy, Scanning Electron Microscopy

Effect of low-power helium-neon laser radiation on the healing of deep second-degree burns in rats

Introduction: This paper presents the results of a study on the effects of two different doses of low-power laser irradiation on healing of deep second degree burns

Material and Methods: 60 rats were randomly allocated to one of four groups. A deep second-degree burn was inflicted in each rat. In the control group [CG] burns remained untreated; in Groups LG1 and LG2 the burns were irradiated with low-power Helium Neon laser with energy densities of 1.2 J/cm[2] and 2.4 J/cm[2] respectively. In the fourth group [G4] the burns were treated topically with 0.2% nitrofurazone cream. The response to treatments was assessed histologically at 7, 16 and 30 days after burning and microbiologically at Day 15

Results: The number of macrophages and the depth of new epidermis was significantly less in the laser treated groups compared to control and nitrofuorazone treated groups. Staphylococcus epidermidis was found in the wounds of all rats in the laser treated groups

Conclusion: Irradiation of deep second-degree burn with low-power laser produced no beneficial effects on healing of burns