Prevalence of Obesity and Screening for Diabetes Among Secondary School Students

Introduction:There is an increasing trend of obesity in children and adolescent globally. The objectives of this study were to identify the prevalence of overweight and obesity among students from secondary schools and to determine the mean random blood sugar (RBS) for the overweight and obese students.Methods:This was a cross sectional study. Two secondary schools that were scheduled for visit by the School Health Team, Taiping in July 2016 were included. A standardized data collection sheet was used to collect the data. Overweight and obesity were defined based on WHO 2007 reference for BMI-for-age criteria. Random blood glucose was checked for overweight and obese students.Results:A total of 184 school students consented and participated. 128 (69.6%) were female and 90 (48.9%) were Malays. The mean weight and height were 56.21 kg and 1.61 m respectively with BMI of 21.49 kg/m2. Overall, the prevalence of obese and overweight were 12.5% and 10.9% respectively. Among the 4 BMI groups, there were no significant difference found in sex (p=0.849) and races (p=0.536). However, there was significant difference (p=0.042) in mean RBS for obese and overweight students between races. RBS readings among overweight and obese students were within normal range with mean of 5.95 (0.67) mmol/l (range between 4.60 – 7.70 mmol/l).Conclusions:The overall prevalence of overweight and obesity were comparable with other studies done in Malaysia. Nevertheless, there was no prevalence of Type II diabetes mellitus among them.

Effects of gap junction blocking on the oxygen partial pressure in acupoints of the bladder meridian / 中国针灸

<p><b>OBJECTIVE</b>To explore the relation between gap junction and meridian phenomenon.</p><p><b>METHODS</b>The oxygen partial pressure in acupoints [see text for formula] and in their corresponding non-acupoints of the Bladder Meridian was observed with the needle-type tissue oxygen tension sensor in the gap junction blocking goats by 1-Heptanol injection and the Connexin 43 (Cx43) gene knockout mice.</p><p><b>RESULTS</b>(1) The oxygen partial pressure in acupoints of Bladder Meridian on goats was higher than that in non-acupoints after 1-Heptanol injection with significant differences between them (both P < 0.01). (2) The oxygen partial pressure in acupoints of Bladder Meridian on goats increased significantly after injecting 1-Heptanol as compare with that either injecting normal saline or injecting nothing with significant differences between them (all P < 0.01). (3) The oxygen partial pressure in acupoints of the Bladder Meridian was significantly higher than that in the non-acupoint controls in Cx43 wild type (WT) mice (all P < 0.01). In Cx43 heterozygote (HT) mice, the oxygen partial pressure between acupoints and non-acupoint controls showed no significant differences (all P > 0.05). (4) In acupoints, the oxygen partial pressure in Cx43 WT mice was significantly higher than that in Cx43 HT mice (all P < 0.05), while in the corresponding non-acupoints, this difference had no statistically significant (all P > 0.05).</p><p><b>CONCLUSION</b>Gap junction maybe the essential factor in signal transduction of acupuncture.</p>

Involvement of connexin 43 in acupuncture analgesia / 中华医学杂志(英文版)

<p><b>BACKGROUND</b>Connexin 43 (Cx43) is one of the major components of human keratinocyte gap junctions. To study whether gap junctional intercellular communication participates in the transfer of acupoint signals and acupuncture analgesia, the expression of Cx43 was studied in Zusanli (ST36) acupoints compared with control non-acupoint regions in rats after acupuncture. In addition, Cx43 heterozygous gene knockout mice were used to further explore the relationship between Cx43 and acupuncture analgesia.</p><p><b>METHODS</b>The expression of Cx43 was detected by immunohistochemistry, immunoblotting, and RT-PCR for the Cx43 protein and mRNA. The influence of the Cx43 gene knockout on acupuncture analgesia was measured by a hot plate and observing the writhing response on Cx43 heterozygous gene knockout mice.</p><p><b>RESULTS</b>Immunohistochemistry showed abundant Cx43 expression in some cells in the skin and subcutaneous tissue of rat ST36 acupoints. The mRNA and protein levels of Cx43 in acupoints were significantly higher than those in the control points in the non-acupuncture group, and even more so after acupuncture. The hot plate and writhing response experiments showed that partial knockout of the Cx43 gene decreased acupuncture analgesia.</p><p><b>CONCLUSION</b>Cx43 expression and acupuncture analgesia showed a positive correlation.</p>

The role of connexin 43 gene in acupuncture analgesia / 中国针灸

<p><b>OBJECTIVE</b>To investigate the possible relationship between the analgesic effect of acupuncture and connexin 43.</p><p><b>METHODS</b>Connexin 43 gene knock-out mice were divided into 4 groups: a wide type (WT) control group, a WT acupuncture group, a heterozygous (HT) control group and HT acupuncture group. Hot-plate test and writhing response induced by acetic acid were used for investigating the analgesic effect of acupuncture.</p><p><b>RESULTS</b>There was no significant difference in the basic pain threshold value between HT and WT mice (P > 0.05). Acupuncture could significantly increase the pain threshold value, prolong the latency period of writhing body and decrease the number of writhing body as compared with pre-acupuncture in WT and HT mice (P < 0.01 or P < 0.05). The pain threshold, latency period of writhing and number of writhing body in HT mice were less than WT mice post-acupuncture (P < 0.05).</p><p><b>CONCLUSION</b>Connexin 43 gene knock-out might partially inhibit the analgesic effect of acupuncture, suggesting that connexin 43 is possibly related with meridians and the effect of acupuncture.</p>