Positive and negative effect of exogenous fat on the bone mineral density in healthy women by dual energy X-ray absorptiometer / 中南大学学报(医学版)

OBJECTIVE@#To examine the influence of exogenous fat (lard) on the bone mineral density (BMD) of lumbar spine measured by dual energy X-ray absorptiometer (DXA) Hologic QDR 4500A in different sites in healthy women.@*METHODS@#Lard packets (5 cm or 10 cm thick) were prepared. The lumbar spine (LS) DXA scans were performed on 37 women(Group A), who were repeated with lard packets (5 cm or 10 cm thick) placed over the abdomen, and the hip DXA scans were performed on 18 women(Group B), who were repeated with lard packets (5 cm or 10 cm thick) placed over the left thigh. Whole body DXA scans were performed on 8 women(Group C), who were repeated with 5 cm thick lard packets over the abdomen. The pair t-test was used to compare the value of BMD with and without lard.@*RESULTS@#After lard was placed over abdomen, the bone area (BA), bone mineral content (BMC), and BMD of the LS decreased significantly (P0.05).@*CONCLUSION@#Lard impacted the BMD by DXA Hologic QDR 4500A, BMD of the LS significantly decreases, but BMD of the whole body has no significant change. BMD of the hip significantly increases with lard.

The effect and mechanism of transient continuous subcutaneous insulin infusion therapy on β cell function, insulin resistance and vascular endothelial injury in newly diagnosed type 2 diabetes / 中华内科杂志

Objectives To explore the effect of transient continuous subcutaneous insulin infusion (CSII) on β cell function, insulin resistance and vascular endothelial injury in newly diagnosed type 2 diabetic patients and its potential mechanism. Methods Ten patients with newly diagnosed type 2 diabetes mellitus (T2DM) accepted CSII for two weeks. Intravenous glucose tolerance test (IVGTT) and hyperinsulinemia euglycemia clamp test were performed before and after CSII. Serum soluble E-selectin (sE-selectin) was used to evaluate the injury of vascular endothelial cell, while serum high sensitivity C reactive protein (hsCRP) and soluble CD_(14) (sCD_(14)) were both used to assess inflammatory condition. Results (1) Compared with those before treatment, the blood glucose levels of IVGTT, the area under the curve of the blood glucose, glycosylated hemoglobin, TC and LDL-C in the patients were decreased after CSII (P < 0. 05 or 0. 01). (2) Compared with those before treatment, the insulin levels of IVGTT (except the fasting insulin), the area under the curve of insulin and acute insulin response were all increased after CSII(P < 0.05 or 0.01). (3) Compared with that before treatment, the glucose infusion ratio in the clamp test [(3.46±1.66)mg·kg~(-1)·min~(-1) increased to (7.14±2.37)mg·kg~(-1)·min~(-1)]and HOMA-β elevated, while HOMA-IR declined (P <0. 05 or 0. 01 in all). (4) Compared with those before treatment, the levels of serum sE-selectin, sCD_(14) and hsCRP were decreased (P < 0. 01, except for hsCRP) . Conclusion Transient intensive insulin therapy in patients with newly diagnosed T2DM is useful to restore 13 cell function, attenuate insulin resistance, repair vascular endothelial injury and improve the disorder of blood sugar and lipid. The mechanism may be related with the inhibition of inflammation in patients.

Effect of exogenous fat on bone mineral density by DXA / 中南大学学报(医学版)

OBJECTIVE@#To observe the effect of exogenous fat on the bone mineral density (BMD) of lumbar spine (LS) on a phantom measured by dual energy X-ray absorptiometry (DXA) Hologic QDR4500A.@*METHODS@#LS DXA scans were performed on an LS phantom, and were repeated when with lard packets (2 fresh lard packets and 2 refined lard packets, 5 cm or 10 cm thick).@*RESULTS@#After fresh lard packets were placed, only L1 BMD decreased significantly (P<0.05).When the 5 cm-thick refined lard packet was placed, BMD, bone mineral content (BMC),and bone area (BA) decreased obviously, but with the 10 cm-thick refined lard packet, BMD, BMC and BA decreased significantly (P<0.05 or P<0.01).@*CONCLUSION@#The exogenous fat may affect LS BMD of lumbar spine phantom by DXA Hologic QDR4500A, with significant decrease of the value,which may cause anomaly measurement of BMD in obese people and even misdiagnosis of osteoporosis.

The synergistic action of estradiol and L-ascorbic acid on the osteoblast-like cell line MG-63 / 中华老年医学杂志

Objective To observe the synergistic action of 17? estradiol(E 2) and L ascorbic acid on the activity of alkaline phosphatase (ALP), cell proliferation and gene expression in osteosarcoma MG 63 cell line, and to study the mechanism of E 2 in treating osteoporosis. Methods MG 63 cells were treated with 10 8 mol/L E 2 with or without 100 ?g/L L ascorbic acid, and then the ALP activity was determined by using ? nitrophenyl phosphate as a substrate. Cell proliferation was measured by ( 3H) thymidine( 3H TdR) incorporation method. mRNA expression of type I collagen, matrix metalloproteinase 1(MMP 1), MMP 2 and osteoprotegerin(OPG) was detected by semiquantitative RT PCR method. Gelatin zymogram was used for the assay of MMP 2 activity. The synergistic action of E 2 and L ascorbic acid on MG 63 cell lines could be evaluated basing on the above indexes since they stand for separately the cell proliferation, gene expression and cell differentiation. Results The action of combined treatment with E 2 and L ascorbic acid on MG 63 cells was obviously stronger than the action using single E 2 or single L ascorbic acid. E 2 and L ascorbic acid increased the ALP activity to 200% ( P

Comprehensive assessment of the ovariectomized rat model of postmenopausal osteoporosis / 中南大学学报(医学版)

Dual-energy X-ray absorptiometry(DEXA),scanning electron microscopy,and bone biomechanical test were used to assess comprehensively bone quantity and quality of ovariectomized rats. In OVX rats,not only bone mineral density (BMD) of lumbar vertebrae in vivo and vitro,but also BMD of femora (except for R3 region) and proximal metaphysis(R1 region) in vitro decreased obviously (P<0.01)，whose bone loss rates of L5 and L6 were the highest and achieved 13%; the trabeculae of OVX rats were few，fine, and discontinued and there were lacunae on the surface;in OVX rats,both compressive strength of vertebral bodies and the mechanical properties of femora decreased;the falling degree of the former was greater;the maximal compressive power of lumbar vertebrae decreased with 33.32%. We conclude that after 4-month post-ovariectomy, the bone quantity and quality of six-month-old rats decreased, especially in the area of abundant cancellous bone such as vertebral bodies,distal femora; the assessment of the efficiency of new drugs to prevent and treat postmenopausal osteoporosis using rat models should include these drugs' effects to the bone mass, the bone structure, and the bone strength.

BILATERAL COMPARISON OF BONE MINERAL DENSITY IN HIP BY DEXA / 中国医师进修杂志

Objective To compare bone mineral density(BMD) at relevant measurement sites between two hips in normal subjects. Methods BMD was measured by dual energy X-ray absorptiometry(DEXA) in the two hips in 70 normal volunteer, 15 to 86 years of age(mean age 53 years). Result Highly significant correlations were found between BMD of the two hips at every measurement sites(r=0.696～0.979, P<0.001),There were small(approximately 2%～3%)but significant differences between BMD of the two hips , The right hip BMD were consistently higher than the left hip BMD at relevant measurement sites (P<0.01)； There were significant positive correlation between BMD and body weight(r=0.289～0.488, P<0.05)except Ward’s triangle. Conclusions BMDs of the two hips are highly correlated at relevant measurement sites; There are strong agreement between BMD of the two hips.

Effect of 17?-estradiol (E_2) on the gene expression of osteoprotegerin in human osteoblast and MG-63 cells / 中华内分泌代谢杂志

Objective To investigate the effects of 17 ? estradiol (E 2) on the gene expression of osteoprotegerin (OPG) in human osteoblast (HOB) and MG 63 osteosarcoma cell lines at various maturity stages of cultures, and to make a better understanding of the mechanism of E 2 deficient osteoporosis. Methods The activities of alkaline phosphatase (ALP), osteocalcin (OC) secreted by cultured cells at different stages of cell proliferation, maturity and collagen mineralization, as well as the effect of E 2 on the OPG gene expression were determined. HOB was prepared from ilial bone tissue in adults, OPG gene expression was quantified by RT PCR, and the activity of ALP, OC was corrected with Bradford′s method. Results For both kinds of cells, expression of OPG at the 12th days′ culture reached the highest levels without any treatment, and 10 -8 mol/LofE 2increasedOPGmRNA to a maximum of about 10 times (P