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1.
Journal of Forensic Medicine ; (6): 193-199, 2023.
Artículo en Inglés | WPRIM | ID: wpr-981854

RESUMEN

Talent is one of the basic and strategic supports for building a modern socialist country in all aspects. Since the 1980s, the establishment of forensic medicine major and the cultivation of innovative talents in forensic medicine have become hot topics in higher education in forensic medicine. Over the past 43 years, the forensic medicine team of Shanxi Medical University has adhered to the joint education of public security and colleges, and made collaborative innovation, forming a training mode of "One Combination, Two Highlights, Three Combinations, Four in One" for innovative talents in forensic medicine. It has carried out "5+3/X" integrated reform, and formed a relatively complete talent training innovation mode and management system in teaching, scientific research, identification, major, discipline, team, platform and cultural construction. It has made a historic contribution to China's higher forensic education, accumulated valuable experience for the construction of first-class major and first-class discipline of forensic medicine, and provided strong support for the construction of the national new forensic talent training system. The popularization of this training mode is conducive to the rapid and sustainable development of forensic science, and provides more excellent forensic talents for national building, regional social development and the discipline construction of forensic science.


Asunto(s)
Humanos , Medicina Legal/educación , Aptitud
2.
Artículo en Chino | WPRIM | ID: wpr-772461

RESUMEN

Human papillomavirus (HPV) is a major causative agent of head and neck squamous cell carcinomas (HNSCC). Over the past several decades, an increasing number of studies established the strong association of HPV with the invasion and metastasis of HNSCC. In the present study, we reviewed the gene mutations in HPV-associated HNSCC and the unique mechanism of E6- and E7-mediated carcinogenesis via interactions with an array of cellular elements. We further discussed the progress in the mechanisms of invasion and metastasis; these mechanisms include non-coding RNAs, deregulating cellular energetics, tumor microenvironment, cancer stem cells, angiogenesis, and lymphangiogenesis.


Asunto(s)
Humanos , Neoplasias de Cabeza y Cuello , Patología , Virología , Invasividad Neoplásica , Metástasis de la Neoplasia , Papillomaviridae , Infecciones por Papillomavirus , Carcinoma de Células Escamosas de Cabeza y Cuello , Patología , Virología
3.
Artículo en Chino | WPRIM | ID: wpr-688036

RESUMEN

Salivary adenoid cystic carcinoma is a very common malignant neoplasm in the salivary glands. One of the most important features of salivary cystic carcinoma is perineural invasion, which renders its complete removal is very difficult through clinical surgery. Consequently, tumor recurrence occurs easily after clinical surgery. Therefore, the molecular mechanisms of perineural invasion in salivary adenoid cystic carcinoma are necessary to explore. These mechanisms are reviewed in this paper.

4.
Journal of Forensic Medicine ; (6): 111-113,119, 2018.
Artículo en Chino | WPRIM | ID: wpr-692391

RESUMEN

Objective To detect the changes of (interleukin, IL) -1α, IL-1β and IL-13 mRNA in lung tissue and serum of drown rats, and to explore the potential value for the diagnosis of drowning in forensic practice. Methods Eighteen SD rats were randomly divided into drowning group, blank control group and myocardial infarction group (as control group). The serum of right ventricular, the inferior lobe of right lung and the myocardium were taken from the rats in different groups. The expressions of IL-1α, IL-1β and IL-13 mRNA in the lung tissue and the serum of right ventricular were detected by TaqMan probe method. Results The expression differences of IL-1α, IL-1β and IL-13 mRNA in lung tissue between drowning group and blank control group, myocardial infarction group were not statistically significant (P>0.05). The expression of IL-1β and IL-13 mRNA in serum of right ventricular increased (P<0.05). The expression differences of IL-1α, IL-1β and IL-13 mRNA in serum between blank con-trol group and myocardial infarction group were not statistically significant (P>0.05). Conclusion The changes of cytokines IL-1β and IL-13 mRNA in the serum of right ventricular of drown rats are statis-tical significance, which are highly correlated with drowning.

5.
Artículo en Inglés | WPRIM | ID: wpr-109415

RESUMEN

Implants have been widely used in restorative treatment for patients who have undergone head and neck cancer surgery. With the development of combination treatment of head and neck cancer, radiotherapy has been a common means of therapy. However, it could induce various changes in hard and soft tissues and reduce the success and survival rate of the implants. Some research, using either animal models or clinical studies, have shown that certain strategies could be used for improving the survival rate of implants. In this review, we discussed the changes in both hard and soft tissues, which may reduce the survival rate of the implants, and the proposed methods for improving the survival rate of patients after radiotherapy.


Asunto(s)
Humanos , Proteínas Morfogenéticas Óseas , Implantes Dentales , Neoplasias de Cabeza y Cuello , Cabeza , Oxigenoterapia Hiperbárica , Métodos , Modelos Animales , Osteorradionecrosis , Radioterapia , Tasa de Supervivencia
6.
Artículo en Inglés | WPRIM | ID: wpr-269662

RESUMEN

microRNAs (miRNAs) are a class of non-coding RNAs that function as endogenous triggers of the RNA interference pathway. Studies have shown that thousands of human protein-coding genes are regulated by miRNAs, indicating that miRNAs are master regulators of many important biological processes, such as cancer development. miRNAs frequently have deregulated expression in many types of human cancers, and play critical roles in tumorigenesis, which functions either as tumor suppressors or as oncogenes. Recent studies have shown that miRNAs are highly related with cancer progression, including initiating, growth, apoptosis, invasion, and metastasis. Furthermore, miRNAs are shown to be responsible for the cancer-related inflammation, anti-cancer drug resistance, and regulation of cancer stem cells. Therefore, miRNAs have generated great interest as a novel strategy in cancer diagnosis and therapy. Here we review the versatile roles of miRNAs in cancers and their potential applications for diagnosis, prognosis, and treatment as biomarkers.


Asunto(s)
Animales , Humanos , Biomarcadores de Tumor , Resistencia a Antineoplásicos , Genética , Transición Epitelial-Mesenquimal , Genética , Regulación Neoplásica de la Expresión Génica , Técnicas de Silenciamiento del Gen , Genes Supresores de Tumor , Inflamación , Genética , MicroARNs , Genética , Fisiología , Invasividad Neoplásica , Genética , Metástasis de la Neoplasia , Genética , Células Madre Neoplásicas , Metabolismo , Oncogenes , Genética
7.
Journal of Forensic Medicine ; (6): 246-249, 2010.
Artículo en Chino | WPRIM | ID: wpr-983571

RESUMEN

OBJECTIVE@#To explore the mRNA expression of microtubule associated protein-2 (MAP-2) after cerebral contusion, and to investigate its relationship with post-injury interval in a rat model of cerebral contusion.@*METHODS@#Based on Feeney's model of cerebral contusion, the time-dependent changes of MAP-2 mRNA were detected by real-time PCR method in different groups.@*RESULTS@#MAP-2 mRNA showed a lower expression in the brain of the control group. The expression of MAP-2 mRNA decreased 1 h post-injury, and reached the lowest level at 6 h, and the expression of MAP-2 mRNA gradually increased in the focus of the cerebral contusion from 12h to 14d after contusion, which kept a high level up to 14 d post-injury.@*CONCLUSION@#The time-dependent changes of MAP-2 mRNA expression may be a new method for estimating the wound age of cerebral contusion in forensic practice.


Asunto(s)
Animales , Femenino , Masculino , Ratas , Lesiones Encefálicas/metabolismo , Contusiones/metabolismo , Modelos Animales de Enfermedad , Patologia Forense , Proteínas Asociadas a Microtúbulos/metabolismo , ARN Mensajero/metabolismo , Ratas Wistar , Reacción en Cadena de la Polimerasa de Transcriptasa Inversa/métodos , Factores de Tiempo
8.
Chinese Journal of Stomatology ; (12): 160-163, 2008.
Artículo en Chino | WPRIM | ID: wpr-235957

RESUMEN

<p><b>OBJECTIVE</b>To investigate the level of urokinase-type plasminogen activator (uPA) and urokinase-type plasminogen activator receptor (uPAR) in synovial fluid of patients with temporomandibular disorders and to analyze their relation with temporomandibular disorders (TMD).</p><p><b>METHODS</b>Synovial fluid was obtained from 64 sides of 56 TMD patients and from 16 sides of 10 asymptomatic healthy volunteers (control). The concentrations of uPA and uPAR in the synovial fluid were measured by ELISA. Forty-eight sides of TMD were divided into 3 groups: arthrosis, structure disorder and osteoarthrosis, each including 16 sides.</p><p><b>RESULTS</b>The levels of uPA and uPAR were significantly higher in the synovial fluid of TMD patients than that in the control group (P < 0.05), and the level of uPA and uPAR in osteoarthrosis group was significantly higher than that in arthrosis and structure disorder group (P < 0.05). However, there was no difference in expression of uPA and uPAR between arthrosis and structure disorder groups (P > 0.05).</p><p><b>CONCLUSIONS</b>uPA and uPAR in the synovial fluid may play a role in the pathogenesis of TMD, and the level of uPA and uPAR in synovial fluid of TMD could be used as a biochemical markers to reflect pathological degree of TMD.</p>


Asunto(s)
Adolescente , Adulto , Anciano , Femenino , Humanos , Masculino , Persona de Mediana Edad , Adulto Joven , Estudios de Casos y Controles , Receptores del Activador de Plasminógeno Tipo Uroquinasa , Metabolismo , Líquido Sinovial , Metabolismo , Trastornos de la Articulación Temporomandibular , Metabolismo , Activador de Plasminógeno de Tipo Uroquinasa , Metabolismo
9.
Artículo en Chino | WPRIM | ID: wpr-249796

RESUMEN

<p><b>OBJECTIVE</b>To examine the relationship between NF-kappaB signal transduction way and the sensitivity of Tca8113 carcinoma cell to Pingyangmycin chemotherapy.</p><p><b>METHODS</b>2 mg/L antisense p65 oligodeoxynucle-otides (5'-GAACAGTTCGTCCATGGCCG-3') was transfected into Tca8113 cells through Lepofectin vectin, then the cells were treated with 8 mg/L Pingyangmycin. After 3 h and 6 h, the expression of p65 in nucleus was studied with immunohistochemical methods and Western blot analyses. After 48 h, the inhibitory rate of cell growth was detected with MTT assay.</p><p><b>RESULTS</b>The NF-kappaB/p65 signal transduction way in Tca8113 cells was activated after treated with Pingyangmycin. But the transfection of antisense p65 oligodeoxynucle-otides inhibited the activation of the signal transduction, the p65 expression in nucleus was decreased significantly (P < 0.05) at 6 h, and the inhibitory rate of cell growth was increased significantly (P < 0.05) at 48 h.</p><p><b>CONCLUSION</b>The results suggested the chemotherapeutic sensitivity of Tca8113 carcinoma cell to Pingyangmycin was improved by the inhibition of NF-kappaB/p65 signal transduction.</p>


Asunto(s)
Humanos , Bleomicina , Línea Celular Tumoral , Proliferación Celular , FN-kappa B , Transducción de Señal , Neoplasias de la Lengua , Factor de Transcripción ReIA , Transfección
10.
Artículo en Chino | WPRIM | ID: wpr-300279

RESUMEN

<p><b>OBJECTIVE</b>To examine the correlation between p65 expression and the metastasis and prognosis in tongue carcinoma.</p><p><b>METHODS</b>The immunohistochemical expression of p65 was examined in 50 cases of tongue carcinoma, and the clinical and histopathologic records were retrospectively analyzed.</p><p><b>RESULTS</b>Overexpression of p65 in cytoplasma and nucleus was found in 64% cases of tongue carcinoma. There was significant difference of p65 expression among different TNM stages, and the higher expression rate of p65 was found in higher stages. Overexpression of p65 was also related to lymph node metastasis. Significantly greater increase in expression levels of p65 was found in the metastatic cases (n = 22) compared to the nonmetastatic cases (n = 10). However, there was no significant difference of p65 expression among different histopathologic grading. The 5-year survival rate of p65 positive and negative group was 59.3% and 88.9% respectively, and there was significant difference in Kaplan-Meier survival curve (chi2 = 5.6, P < 0.05).</p><p><b>CONCLUSION</b>The results suggest that the expression of p65 was important to predict the metastasis and prognosis in tongue carcinoma.</p>


Asunto(s)
Adulto , Anciano , Femenino , Humanos , Masculino , Persona de Mediana Edad , Metástasis Linfática , Pronóstico , Estudios Retrospectivos , Neoplasias de la Lengua , Factor de Transcripción ReIA
11.
Artículo en Chino | WPRIM | ID: wpr-300334

RESUMEN

<p><b>OBJECTIVE</b>To observe the expression of urokinase-type plasminogen activator (uPA) in human tongue squamous carcinoma cells (Tca8113) under hypoxia, in order to explore the relation between hypoxia and invasion and metastasis of oral cancer.</p><p><b>METHODS</b>Under different hypoxic times(0, 3, 6, 12, 24 h), the expression of uPA protein was examined quantitatively using immunohistochemical technique (IH) and flow cytometry (FCM).</p><p><b>RESULTS</b>Hypoxia promoted the expression of uPA. The longer hypoxic time was, the higher expression of uPA was.</p><p><b>CONCLUSION</b>Hypoxia can promote invasion and metastasis of oral squamous carcinoma through stimulating the expression of uPA.</p>


Asunto(s)
Humanos , Carcinoma de Células Escamosas , Neoplasias de la Boca , Invasividad Neoplásica , Neoplasias de la Lengua , Activador de Plasminógeno de Tipo Uroquinasa
12.
Artículo en Chino | WPRIM | ID: wpr-319044

RESUMEN

<p><b>OBJECTIVE</b>To study the effect of phosphorothioate antisense Bcl-xL oligodeoxynucleotides on apoptosis and thermosensitivity of BcaCD885 cells.</p><p><b>METHODS</b>After phosphorothioate antisense Bcl-xL oligodeoxynucleotides were transfected into BcaCD885 cells. The characteristics of apoptotic cells were evaluated by morphological observation and TUNEL staining. Apoptotic rate and Bcl-xL protein expression were analyzed with flow cytometry. The influence of phosphorothioate antisense Bcl-xL oligodeoxynucleotides on apoptotic rate of BcaCD885 cells induced by hyperthermia with 43 degrees C 40 min was also examined through flow cytometry.</p><p><b>RESULTS</b>The BcaCD885 cells transfected with phosphorothioate antisense Bcl-xL oligodeoxynucleotides displayed apoptotic morphological features. The Bcl-xL protein expression level of these cells was down-regulated significantly compared with the controlled group (P < 0.05). The apoptotic rate of these cells induced by hyperthermia was increased significantly compared with the controlled group (P < 0.05).</p><p><b>CONCLUSION</b>Phosphorothioate antisense Bcl-xL oligodeoxynucleotides can induce apoptosis and improve thermosensitivity of BcaCD885 cells.</p>


Asunto(s)
Humanos , Apoptosis , Citometría de Flujo , Regulación Neoplásica de la Expresión Génica , Genética , Calor , Hipertermia Inducida , Neoplasias de la Boca , Genética , Patología , Oligodesoxirribonucleótidos Antisentido , Farmacología , Oligonucleótidos Antisentido , Genética , Farmacología , Proteínas Proto-Oncogénicas c-bcl-2 , Genética , Metabolismo , Tionucleótidos , Farmacología , Transfección , Células Tumorales Cultivadas , Proteína bcl-X
13.
Artículo en Chino | WPRIM | ID: wpr-319060

RESUMEN

<p><b>OBJECTIVE</b>The purpose of this article was to observe the effect of by microwave thermocoagulation on oral benign tumor and to provide theoretical basis for clinic treatment of oral benign tumor.</p><p><b>METHODS</b>165 patients with oral benign tumor were included. There were 83 patients with oral bursa cyst, 42 patients with hemangioma, 19 patients with papilloma, 14 patients with fibrous hyperplasia and 7 patients with epulis. All the patients were treated by WB-100 microwave thermocoagulation.</p><p><b>RESULTS</b>144 patients were effective after the first treatment and 21 patients were effective after the second treatments. No side effect and reoccurrence were found after 3 years.</p><p><b>CONCLUSION</b>Microwave thermocoagulation is an effective and safe method in clinic treatment of oral benign tumor.</p>


Asunto(s)
Adolescente , Adulto , Niño , Femenino , Humanos , Masculino , Persona de Mediana Edad , Electrocoagulación , Métodos , Hemangioma , Radioterapia , Cirugía General , Microondas , Usos Terapéuticos , Enfermedades de la Boca , Radioterapia , Cirugía General , Neoplasias de la Boca , Radioterapia , Cirugía General , Mucocele , Radioterapia , Cirugía General
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