Effect of silencing heparanase on the biological behavior of gastric carcinoma in nude mice / 中华肿瘤杂志

<p><b>OBJECTIVE</b>To investigate the effects of silencing heparanase (HPA) on growth, angiogenesis and metastasis of human gastric carcinoma transplanted in nude mice.</p><p><b>METHODS</b>Human gastric carcinoma SGC-7901 cells and those cells with silenced HPA (gastric carcinoma SGC-7901-HPA(-)) were separately transplanted subcutaneously in 6 nude mice. The time, size and speed of tumor growth were recorded. RT-PCR and Western-blot were used to detect the expression of HPA mRNA and protein in the subcutaneous tumors of the two groups. Immunohistochemical staining was used to detect microvessel density (MVD) in the subcutaneous tumors of the two groups. Cells of the subcutaneous transplanted tumors of the two groups were separately injected into the peritoneal cavity of nude mice, 6 mice each. The growth of metastatic tumors in nude mice was observed.</p><p><b>RESULTS</b>Human gastric carcinoma SGC-7901 cells and SGC-7901-HPA(-) cells were subcutaneously inoculated in nude mice, and tumors appeared at 4 days and 7 days after inoculation, respectively. The MVD was (20.69 ± 1.20)/HP and (11.35 ± 1.94)/HP, respectively (P < 0.05). The expressions of HPA mRNA and protein of the subcutaneously transplanted SGC-7901-HPA(-) tumor were decreased. Four voluminous metastatic tumors caused by SGC-7901 cells occurred in 3 mice in the liver, right kidney, omentum and intestine. Two smaller abdominal metastatic tumors of SGC-7901-HPA(-) cells were found in the liver and right kidney.</p><p><b>CONCLUSION</b>Silencing HPA can inhibit the tumor growth, angiogenesis and metastasis of human gastric cancer in nude mice. It suggests that HPA might become a new target for prevention and treatment of gastric cancer.</p>

The diagnostic values of MRI, enhanced CT and PET-CT in detecting skull base invasion of nasopharyngeal carcinomas: A comparative study / 肿瘤

Objective: To compare the diagnostic values of magnetic resonance imaging (MRI), enhanced computed tomography (CT), and positron emission tomography (PET)-CT using 18F-fluoro-2-deoxyglucose (FDG) in detecting skull base invasion of nasopharyngeal carcinoma (NPC) and provide the evidence for differentiated diagnosis of the skull base invasion of NPC. Methods: The fifty seven patients were scanned by MRI, enhanced CT, and PET-CT. The three imaging examinations were finished within 20 days. The diagnosis standards were based on histopathologic findings or clinical and imaging follow-up results within 6 months. The sensitivity, specificity, accuracy, positive predictive value (PPV) and negative predictive value (NPV) of the three scanning technologies were compared. Results: For detecting skull base invasion of NPC, the sensitivity of enhanced CT, MRI, and PET-CT were 68.18%, 84.09%, and 97.67%, respectively; the specificity were 76.92%, 69.23%, and 57.14%, respectively; the accuracy were 70.18%, 80.70%, and 87.72%, respectively; PPV were 90.90%, 90.24%, and 87.50%, respectively; NPV were 41.67%, 56.25%, and 88.89%, respectively. PET-CT was better than enhanced CT in sensitivity, accuracy, and NPV (P <0.05). It was also better than MRI in sensitivity and NPV (P <0.05). Conclusion: Among three imaging technologies,PET-CT has obvious advantage in detecting skull base invasion of NPC patients, especially for new patients.