RESUMEN
This study is to examine the effects of NNIspm-mediated cellular senescence of HepG2 cells and elucidate its potential molecular mechanism. Cellular senescence was detected with senescence-associated beta-galactosidase staining. Cell cycle distribution, intracellular fluorescence intensity and accumulation of intracellular reactive oxygen species (ROS) were detected by high content screening (HCS). Protein expression was detected by Western blotting. Polyamines content was analyzed by high performance liquid chromatography (HPLC). The results demonstrated that NNIspm significantly induced HepG2 cells senescence. This effect was due to the decrease of intracellular polyamines, the arrest at G0/G1 phase and an increase of ROS level. The molecular senescence marker p21 increased significantly after NNIspm treatment. In contrast, the protein expressions of Cyclin E and CDK2 were obvious down-regulation. The results indicated that cellular senescence induced by NNIspm was one of its antitumor mechanisms.
Asunto(s)
Humanos , Antineoplásicos , Metabolismo , Farmacología , Senescencia Celular , Ciclina E , Metabolismo , Quinasa 2 Dependiente de la Ciclina , Metabolismo , Inhibidor p21 de las Quinasas Dependientes de la Ciclina , Metabolismo , Fase G1 , Células Hep G2 , Proteínas Oncogénicas , Metabolismo , Poliaminas , Metabolismo , Farmacología , Especies Reactivas de Oxígeno , MetabolismoRESUMEN
The objective of this study is to examine the effects of ANISpm, a novel polyamine naphthalimide conjugate, with acetylsalicylic acid against hepatocellular carcinoma in vivo and in vitro and elucidate its potential molecular mechanism. The proliferation inhibition was detected by MTT assay. Cell apoptosis, intracellular fluorescence intensity and mitochondrial membrane potential (MMP) were detected by high content screening (HCS) analysis. Polyamines content was analyzed by reverse-phase high performance liquid chromatography Protein expression levels were quantified by Western blotting assay. The combination treatment strongly inhibited cell proliferation, induced cell apoptosis in HepG2 cells and H22 hepatoma cells, which was mediated by enhanced ANISpm uptake via up-regulation of spermidine/spermine N1-acetyltransferase (SSAT) and depression of intracellular polyamine. Furthermore, this synergistic apoptosis was involved in mitochondria and death-receptor signal pathway. All these findings demonstrated that the combination treatment with acetylsalicylic acid and ANISpm resulted in synergistic antitumor effects on hepatoma cells. Thus, combination therapy with these agents may be useful as a potential template for the development of better chemotherapeutic strategy against hepatoma.
Asunto(s)
Animales , Femenino , Humanos , Ratones , Acetiltransferasas , Metabolismo , Antineoplásicos , Farmacología , Apoptosis , Aspirina , Farmacología , Caspasa 8 , Metabolismo , Caspasa 9 , Metabolismo , Línea Celular Tumoral , Proliferación Celular , Sinergismo Farmacológico , Células Hep G2 , Neoplasias Hepáticas Experimentales , Patología , Potencial de la Membrana Mitocondrial , Naftalimidas , Metabolismo , Farmacología , Trasplante de Neoplasias , Poliaminas , Metabolismo , Farmacología , Distribución Aleatoria , Espermina , Metabolismo , Farmacología , Carga Tumoral , Regulación hacia ArribaRESUMEN
This paper is to report the study of resveratrol-induced apoptosis and its mechanisms in MCF-7 cells. MTT assay was performed to assess the cytotoxicity of resveratrol on MCF-7 cells. Hoechst 33258 staining was used to observe cellular morphologic changes in apoptosis. Apoptosis was measured by flow cytometric analysis and the protein expression was examined by Western blotting analysis. The results indicated that resveratrol could inhibit MCF-7 cell growth in a time- and concentration-dependent manner. Remarkable morphologic changes in the cells after 60 micromol L(-1) resveratrol treatment, including cell nuclear shrinkage, DNA condensation and apoptotic bodies, were observed by Hoechst 33258 staining. Resveratrol could induce apoptosis and activate p38 and p53 in a time dependent manner in MCF-7 cells. In addition, the cell growth inhibitory ratio and the apoptotic ratio of resveratrol-treated group decreased markedly by the p38 MAPK inhibitor SB203580 or p53 inhibitor pifithrin-alpha. Further experiments confirmed that resveratrol-induced p53 activation was reduced by SB203580 whereas the activation of p38 was not affected by pifithrin-alpha. In conclusion, resveratrol induced apoptosis in MCF-7 cells could be through activating p38-p53 signal pathway.
Asunto(s)
Humanos , Antineoplásicos Fitogénicos , Farmacología , Apoptosis , Benzotiazoles , Farmacología , Proliferación Celular , Relación Dosis-Respuesta a Droga , Inhibidores Enzimáticos , Farmacología , Imidazoles , Farmacología , Células MCF-7 , Piridinas , Farmacología , Transducción de Señal , Estilbenos , Farmacología , Tolueno , Farmacología , Proteína p53 Supresora de Tumor , Metabolismo , Proteínas Quinasas p38 Activadas por Mitógenos , MetabolismoRESUMEN
In the present study, the apoptotic mechanism and polyamine transporter recognition of WJH-6, a novel polyamine conjugate, were investigated in K562 and HL-60 cells. The cytotoxicity of WJH-6 was assessed by MTT assay; cell cycle distribution and apoptosis were measured by flow cytometry; the protein expression of Caspase-3, Caspase-8, Caspase-9, Bid and mitochondrial membrane potential (MMP) were evaluated by high content screening (HCS) analysis; the protein expression of cytochrome c was measured by Western blotting. The results showed that WJH-6 could be recognized and transported by polyamine transporter (PAT). Furthermore, WJH-6 was able to inhibit K562 and HL-60 cells proliferation and induce apoptosis. This apoptotic effect was relative to MMP loss, cytochrome c release from mitochondria to cytoplasm and the activation of Caspase-8, Caspase-9, Caspase-3 and Bid. These results suggested that WJH-6-induced K562 and HL-60 cells apoptosis was related with mitochondrial damage.