Effect of patent foramen ovale on development of post-operative stroke in patients undergoing non-cardiac surgery: a meta-analysis / 中华麻醉学杂志

Objective:To evaluate the effect of patent foramen ovale on the development of post-operative stroke in the patients undergoing non-cardiac surgery using a meta-analysis approach.Methods:A comprehensive search was conducted across multiple databases including PubMed, Embase, Web of Science, CINAHL, Cochrane Library, China Biomedical Literature Database, Wanfang Data Knowledge Service Platform, and China Journal Full Text Database.The inclusion criteria encompassed studies assessing the correlation between patent foramen ovale and post-operative stroke.The primary outcome measure focused on the incidence of post-operative stroke, and secondary outcome measures comprised mortality, myocardial infarction rate, and readmission rate within 30 days after surgery. The quality of literature meeting the inclusion criteria was evaluated and data were extracted, and then meta-analysis was conducted using RevMan 5.4 software.Results:Eight retrospective cohort studies involving 21 142 237 patients were included.The results of meta-analysis showed that patent foramen ovale was associated with post-operative stroke and readmission within 30 days after surgery.There were no significant differences in all-cause mortality and myocardial infarction rates between patent foramen ovale group and mon-patent foramen ovale group ( P>0.05). Conclusions:Patent foramen ovale can increase the risk of post-operative stroke in the patients undergoing non-cardiac surgery.

Application of contrast-enhanced ultrasonography in improving the success rate of percutaneous biopsy for peripheral pulmonary lesions / 介入放射学杂志

Objective To investigate the clinical application of contrast enhanced ultrasonography (CEUS) in performing percutaneous biopsy for peripheral lung lesions. Methods A total of 132 patients with peripheral lung lesions underwent ultrasonography-guided percutaneous biopsy. Of the 132 patients, 72 received CEUS examinations before biopsy (CEUS group), and 60 received simple biopsy with no CEUS examination (control group). The ultrasonography imaging characteristics, the times of puncture biopsy, the diagnostic accuracy rate and the incidence of complications were recorded , and the results were compared between the two groups. The clinical value of CEUS in improving the success rate of percutaneous biopsy for peripheral pulmonary lesions was analyzed. Results Necrosis(areas of echoless) was demonstrated in 44.4%of the patients (32/72) in CEUS group, while it was displayed in only 6.7% of the patients (4/60) in the control group. The demonstration rate of necrosis in CEUS group were statistically higher than that in the control group (P <0.01). In CEUS group coexisting local pulmonary atelectasis was found in 12.5%of the patients (9/72), and the pulmonary lesion within the atelectasis could be clearly displayed. In the control group, local pulmonary atelectasis was demonstrated only in 1.7% of patients (1/60), the difference between the two groups was statistically significant (P<0.05). The diagnostic accuracy rate of CEUS group and the control group was 95.8% (69/72) and 80% (48/60) respectively, the difference was significant (P<0.01). The average number of punctures in CEUS group and the control group was (2.4 ± 0.6) and (2.6 ± 0.6) times respectively, the difference was not significant. The incidence of complications in CEUS group and the control group was 2.8% and 3.3%respectively, the difference was not significant. No severe complications occurred in both groups. Conclusion CEUS examination that is carried out before percutaneous pulmonary biopsy can effectively demonstrate the inner structure of the lesion, thus the diagnosis can be correctly made. Therefore, CEUS is of great value in guiding percutaneous pulmonary biopsy.

Quantity change and clinical significance of CD14- CD11b+ CD33+ myeloid-derived suppressor cells in patients with multiple injury / 中华创伤杂志

Objective To explore the quantity change and significance of CD14-/CD11b+/CD33 + myeloid-derived suppressor cells (MDSCs) in patients with multiple injury. Methods Thirtyfour patients with multiple injury and seven healthy volunteers were enrolled in this study. Peripheral blood was collected and the factors of CD14-/CD1 1 b+/ CD33 + were taken as markers of MDSCs. The percentage of MDSCs was determined by flow cytometry (FCM) and serum interleukin-10 and C-reactive protein levels were determined by ELISA to analyze the quantity change and clinical significance of MDSCs. Results The percentage of MDSCs in peripheral blood of healthy volunteers was (1.13 +0. 25) %. At days 1,2, 3 and 7 after injury, the percentage of MDSCs in peripheral blood were (1.20 +0.22) %, (6.44 + 0.35) %, (13.84 ± 2.07) % and (15.60 ± 1.63) % respectively in patients with infection and multiple injury, whereas (1.29 ±0. 30)%, (4.93 +0. 32)%, (5.15 ±0. 21)% and (3.77 ± 0.34) % respectively in patients without infection. The percentages of MDSCs in two groups showed significant differences at days 2, 3 and 7 after trauma (P＜0.05). No correlation was found between MDSCs percentage in peripheral blood and injury severity score, serum interleukin-10 or C reactive protein in patients with multiple injury (P ＞ 0.05). Conclusions The increase of proportion ofMDSCs in peripheral blood correlates with the onset of infection in patients with multiple injury, indicating that the expansion of MDSCs in peripheral blood may play important roles in immune dysfunction after multiple injury.

Preparation and characterization of polyclonal antibodies against VLDL receptor / 华中科技大学学报（医学）（英德文版）

The polyclonal antibodies against VLDL receptor were prepared and identified. Rabbits were immunized with polypeptide fragment of VLDL receptor as antigen. The collected blood serum of the immunized rabbits was analyzed and identified by using ELISA and Western Blot. The results showed that the rabbit against mouse and human VLDL receptor antibodies were obtained with high titer and could recognize the natural VLDL receptors through Western blot. The prepared polyclonal antibodies against VLDL receptor provide a new tool to study the protein of VLDL receptor.

Preparation and characterization of polyclonal antibodies against VLDL receptor / 华中科技大学学报（医学）（英德文版）

The polyclonal antibodies against VLDL receptor were prepared and identified. Rabbits were immunized with polypeptide fragment of VLDL receptor as antigen. The collected blood serum of the immunized rabbits was analyzed and identified by using ELISA and Western Blot. The results showed that the rabbit against mouse and human VLDL receptor antibodies were obtained with high titer and could recognize the natural VLDL receptors through Western blot. The prepared polyclonal antibodies against VLDL receptor provide a new tool to study the protein of VLDL receptor.

Expression of TREM-1 in patients with biliary infection / 中国普通外科杂志

Objective To investigate the expression and significance of human TREM-1 mRNA in patients with (biliary) infection. Methods Peripheral blood of 32 patients with biliary infection and 7 healthy volunteers were (collected). TREM-1 mRNA was determined by semi-quantitative RT-PCR. TNF-? was determined by ELISA method. Results The values of TREM-1/?-actin of control group was 0.48?0.072, while those of biliary infection group in 1d, 2d, 3d, 7d were 0.93?0.070,0.90?0.060,0.82?0.092,0.66?0.062 respectively (P