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1.
Chinese Journal of Cancer ; (12): 121-129, 2013.
Artículo en Inglés | WPRIM | ID: wpr-295839

RESUMEN

Apoptosis and autophagy are important molecular processes that maintain organismal and cellular homeostasis, respectively. While apoptosis fulfills its role through dismantling damaged or unwanted cells, autophagy maintains cellular homeostasis through recycling selective intracellular organelles and molecules. Yet in some conditions, autophagy can lead to cell death. Apoptosis and autophagy can be stimulated by the same stresses. Emerging evidence indicates an interplay between the core proteins in both pathways, which underlies the molecular mechanism of the crosstalk between apoptosis and autophagy. This review summarizes recent literature on molecules that regulate both the apoptotic and autophagic processes.


Asunto(s)
Animales , Humanos , Apoptosis , Proteínas Reguladoras de la Apoptosis , Metabolismo , Autofagia , Homeostasis , Neoplasias , Metabolismo , Patología , Transducción de Señal
2.
Chinese Journal of Biotechnology ; (12): 437-440, 2004.
Artículo en Chino | WPRIM | ID: wpr-249967

RESUMEN

To examine if polyprotein gene (VP2/VP4/VP3) of Infectious Bursal Disease Virus (IBDV) could be delivered into mammalian cells and expressed using attenuated Salmonella typhimurium as vector. The IBDV polyprotein gene was amplified by RT-PCR and inserted in to pCI, an eukaryotic expression plasmid. The resulting recombinant pCI-VP2/VP4/VP3 was transformed by electroporation into attenuated Salmonella typhimurium strain ZJ111 (dam- and phoP-), which was then use to transfect the Vero cells. Gene specific RT-PCR revealed that VP2/VP4/VP3 was transcribed into mRNA in the Vero cells. Indirect immunofluorscence assay, SDS-PAGE and Western-blot analysis showed that VP2/VP4/VP3 was expressed and the product was immuno-reactive with anti-IBDV serum. This work provides essential precondition for developing a new oral DNA vaccine against IBDV.


Asunto(s)
Animales , Chlorocebus aethiops , Electroporación , Vectores Genéticos , Genética , Virus de la Enfermedad Infecciosa de la Bolsa , Genética , Metabolismo , Poliproteínas , Genética , Reacción en Cadena de la Polimerasa de Transcriptasa Inversa , Salmonella typhimurium , Genética , Metabolismo , Transfección , Células Vero , Proteínas Virales , Genética
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