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1.
Chinese Journal of Virology ; (6): 178-184, 2009.
Artículo en Chino | WPRIM | ID: wpr-334754

RESUMEN

Occult hepatitis B virus (HBV) infection status of blood donors in a southern city in China was investigated by immunological assays and nucleic acid testing. Overall, 17 (0.19%, 95% CI: 0.11%-0.30%) of the 9023 HBsAg negative samples were found to be positive for the presence of HBV DNA. "A" epitope sequences were obtained from 14 among them. Mutation(s) in aa124-aa147 existed in 6 (42.9%, 6/14) samples and 4 (66.7%, 4/6)were G145R mutation. Ratio of genotype C in occult donors (10/17) was statistically higher than HBs-positive donors (0/15, P<0.01), which implied that HBV genotype C leaded to occult infection more easily.


Asunto(s)
Adolescente , Adulto , Femenino , Humanos , Masculino , Adulto Joven , Donantes de Sangre , China , Epidemiología , ADN Viral , Genética , Genotipo , Hepatitis B , Epidemiología , Alergia e Inmunología , Virología , Antígenos de Superficie de la Hepatitis B , Genética , Alergia e Inmunología , Virus de la Hepatitis B , Clasificación , Genética , Alergia e Inmunología , Fisiología , Pruebas Inmunológicas , Mutación , Alineación de Secuencia , Análisis de Secuencia de ADN
2.
Chinese Medical Journal ; (24): 19-23, 2004.
Artículo en Inglés | WPRIM | ID: wpr-235840

RESUMEN

<p><b>BACKGROUND</b>Chronic hypoxia can cause pulmonary hypertension and pulmonary heart disease with high mortality. The signal transduction pathway of protein kinase C (PKC) plays an important role in chronic pulmonary hypertension. So it is necessary to investigate the effect of PKC on voltage-gated potassium (K+) channels in pulmonary artery smooth muscle cells of rats exposed to chronic hypoxia.</p><p><b>METHODS</b>Male Wistar rats were randomly divided into a control group (group A) and a chronic hypoxia group (group B). Group B received hypoxia [oxygen concentration (10 +/- 1)%] eight hours per day for four consecutive weeks. Single pulmonary artery smooth muscle cells were obtained using an acute enzyme separation method. Conventional whole cell patch clamp technique was used to record resting membrane potential, membrane capacitance and voltage-gated K+ currents. The changes in voltage-gated K+ currents before and after applying paramethoxyamphetamine (PMA) (500 nmol/L), an agonist of PKC, and PMA plus carbohydrate mixture of glucose, fructose and xylitol (GFX) (30 nmol/L), an inhibitor of PKC, were compared between the two groups.</p><p><b>RESULTS</b>The resting membrane potential in group B was significantly lower than that of group A: -(29.0 +/- 4.8) mV (n = 18) vs -(42.5 +/- 4.6) mV (n = 35) (P < 0.01). But there was no change in membrane capacitance between the two groups: (17.9 +/- 4.6) pF (n = 40) vs (19.7 +/- 5.8) pF (n = 31) (P > 0.05). The voltage-gated K+ currents were significantly inhibited by PMA in group A, and this effect was reversed by GFX. However, the voltage-gated K+ currents in group B were not affected by PMA.</p><p><b>CONCLUSIONS</b>The resting membrane potential and voltage-gated K+ currents in pulmonary artery smooth muscle cells from rats exposed to chronic hypoxia decreased significantly. It seems that PKC has different effects on the voltage-gated K+ currents of pulmonary artery smooth muscle cells under different conditions.</p>


Asunto(s)
Animales , Masculino , Ratas , Enfermedad Crónica , Hipoxia , Miocitos del Músculo Liso , Fisiología , Canales de Potasio con Entrada de Voltaje , Fisiología , Proteína Quinasa C , Fisiología , Arteria Pulmonar , Distribución Aleatoria , Ratas Wistar
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