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【Objective】 To investigate the effects of high-dose hyperbaric trioxygen autologous blood therapy (HOT) on oxygenation index (PaO2/FiO2) and serum inflammatory factors in dogs with acute respiratory distress syndrome (ARDS). 【Methods】 Twelve healthy adult beagles were randomly divided into 3 groups (n=4). The blank group was injected with normal saline intravenously. The ARDS model was established by intravenous injection of oleic acid (0.12 mL/kg) in the ARDS group and ARDS+ HOT group. The mark of a successful model is that the oxygen and index (PaO2/FiO2) <300 mmHg. In the ARDS+ HOT group, after the ARDS model was established, 16 G indwelling needle was used to puncture the left femoral vein and connect the line of the HOT device. Venous blood (50 mL/ dog) was collected from the femoral vein under negative pressure to the blood storage bottle (100 mL blood storage bottle), and then the blood collection was stopped and the gas injection switch of the HOT device was turned on. Inject 50 mL of 20ng/dL trioxygen gas into the blood storage bottle. After gas injection, turn the blood storage bottle upside down three times to fully trioxidize the blood and then inject it back into the dog. Repeat this treatment for 10 cycles. PaO2 and PaO2/FiO2 were detected before treatment and at 1, 2, 3, 4, 5 h after treatment. The serum was retained after treatment, and the expressions of inflammatory cytokines (IL-6, IL-8) and myeloperoxidase (MPO) were detected by ELISA. The animals were euthanized, and the gross lung morphology of the dogs was observed at autopsy. The dorsal segment of the left lower lobe of the lung was taken for pathological section HE staining, and the morphological changes of the lung tissue were observed under the microscope. 【Results】 After 5 hours of treatment, the PaO2/FiO2 of blank group was 481.85±35.31, and that of ARDS group was 183.67±20.18, which was significantly lower than that of blank group (P<0.01). The ARDS HOT group was 271.90±21.35, which was significantly higher than the ARDS group (P<0.01). The inflammatory factor IL-6 was (206.49±38.85) pg/mL in the blank group, and (293.12±30.38) pg/mL in the ARDS group, which was significantly higher than that in the blank group (P<0.01). There was a significant difference between the ARDS HOT group and ARDS group (221.56±46.69) pg/mL (P<0.01). The results of inflammatory factor IL-8 detection showed that the IL-8 in ARDS group was increased compared with the blank group (P<0.01); and the IL-8 in ARDS HOT group was decreased compared with ARDS group (P<0.01). Myeloperoxidase MPO test results showed that the blank group was (505.58±73.94) pg/mL, and the ARDS group was (605.69±108.88) pg/mL, which was significantly higher than the blank group (P<0.05). The ARDS HOT group was (476.52±103.85) pg/mL, which was significantly lower than the ARDS group (P<0.05). Microscopic examination of lung pathology showed that the lung tissue injury in ARDS HOT group was significantly reduced compared with ARDS group. 【Conclusion】 HOT can reduce the inflammation and injury of lung in ARDS model dogs through significantly increasing the PaO2/FiO2, down-regulating the expression of MPO, then inhibiting the activity of neutrophils and reducing the levels of IL-6 and IL-8.
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Autologous ozonized blood transfusion(AOBT) is a therapy of re-transfusion of 100-200 mL of autologous blood after shaking and agitation with appropriate amount of oxygen-ozone in vitro. The oxidation of blood through the strong oxidation of ozone can enhance the non-specific immune response of the body, regulate the internal environment and promote health. This therapy has been increasingly applied in clinical practice, while no unified standard for the operation process in terms of ozone concentration, treatment frequency and treatment course had been established. This operation process of AOBT is primarily explored in order to standardize the operation process and ensure its safety and efficacy.
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【Objective】 To investigate the effect of electrolyte concentration on transfusion related complications in patients undergoing cardiac surgery after short-term massive transfusion (MT). 【Methods】 Seventy-six cases of adult cardiac surgery in our hospital from January 2018 to December 2019 were collected. According to their perioperative blood transfusion units, they were divided into the massive blood transfusion group (red blood cell units≥ 10 U/ person) and the control group[red blood cell units (0~1)U/ person]. The concentrations of Na+ , K+ and Ca2+ ions in serum were detected by ISE (ion selective electrode method) and NM-BAPTA before and after intraoperative blood transfusion. The changes of Na+ , K+ and Ca2+ ions in serum before and after intraoperative blood transfusion were analyzed and compared through T-test. 【Results】 There were 38 patients in the MT group and 38 in the control group, and the demographic variables and types of surgeries (number) of patients in the two groups were comparable (P>0.05). The serum K+ , Ca2+ and Na+ before operation were similar (P>0.05), and within the normal range. The mean values of serum K+ , Ca2+ and Na+ (mmol/l) in the massive blood transfusion group and the control after operation (blood transfusion) were 4.25±0.44 vs 4.01±0.53, 2.31±0.12 vs 2.45±0.43, 140.82±2.31 vs 146.44±4.35 (P<0.05). In the control, the serum K+ , and Ca2+ were slightly lower than those before operation, while the Na+ was slightly higher (all P>0.05), and all were within the normal range. 【Conclusion】 Patients undergoing cardiac surgery are prone to suffer electrolyte disorders after massive blood transfusion. Electrolyte concentration should be monitored in time during and after perioperative blood transfusion.
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Objective To demonstrate the influence of stress on attentional bias in athletes,in order to make guidance for athletes' attention training and control.Method Using dot-probe paradigm,we analyzed the attention behavior of 25 athletes either under stress or not when viewing different pictures of emotional face.The reaction time and the score of the attention bias were recorded and compared.Result The reaction time to negative face pictures under stress was significantly shorter than that to the positive and neutral ones.The value of the attention bias to negative pictures was significantly bigger than O.Conclusion Stress tends to induce attention bias of athletes to negative information.
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Increasing evidences have shown that intestinal microbiota may contribute to the etiology of colorectal cancer(CRC). Studies have shown some microbial species which can increase the risk of CRC. Intestinal bacteria can promote carcinogenesis by affecting DNA integrity,regulating immune reaction,inducing inflammation,increasing cell proliferation and altering stem cell dynamics. Modulation of intestinal microbiota may become an approach to prevent or treat CRC. This article reviewed the effect and mechanism of intestinal bacteria flora on CRC.
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Recent meta-analysis based on both clinical trials and epidemiological studies has revealed the relationship of dietary cholesterol,blood cholesterol level and cholesterol lowering drugs (statins) with colorectal cancer.This review summarises the advances in current evidences of linking cholesterol metabolism and risk of colorectal cancer and the underlying molecular mechanisms based on pharmacogenetics and molecular pathology.
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Objective This study is to examine the effect of different breathing patterns (abdominal respiration,natural respiration)on skin temperature(TEMP),skin conductivity(SC) and heart rate variability (HRV) in college students.Methods We use single blind trial in the study,and randomly select thirty students,including fourteen male and sixteen female,aged 19.6±0.77 years,and then randomly divide them into two groups:the intervention group (abdominal respiration group,6 males and 9 females) and the control group(natural respiration group,8 males and 7 females).In the study three phases are included:baseline phase(five minutes),test phase(fifteen minutes),recovery phase(five minutes).Data of above indicators was collected at 3-5 min,14.5-19.5 min,and 23-25 min,respectively,during the study.Results (1) There are no significant difierences of all three indicators between the two groups in the baseline phase(P0.05).(3)There are no significant differences of TEMP and the HRV in the recovery phase between the two groups(P>0.05),except the SC (P<0.05).Conclusion No significant difference is observed between the influences of abdominal and natural respiration on the emotion-related biological indicators.
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AIM: To explore the effect of complement on the cerebral ischemia/reperfusion injury in rat and the protection by sCR1-SCR15-18. METHODS: 75 male SD rats were randomly divided into three groups: sham operation group (SO, n=15), middle cerebral artery occlusion and reperfusion (MCAO) without treatment group (I/R, n=30); MCAO treated with sCR1-SCR15-18 group (sCR1-SCR15-18, n=30). After the MCAO for 2 h, then reperfusion for 24 h, the scores of neural behavioral functional deficits were determined. Infarction area was measured by TTC staining. Activity of MPO in cerebral cortex was detected. C3b deposition and pathological change were observed by immunohistochemial staining and HE staining, respectively. RESULTS: After reperfusion for 24 h, the neurological deficits score, infarction area and activity of MPO in sCR1-SCR15-18 group were decreased compared to I/R group. In sCR1-SCR15-18 group, C3b deposition in ischemic area was decreased and pathological injury was improved compared to I/R group. CONCLUSION: Complement plays a role in cerebral ischemia-reperfusion injury and sCR1-SCR15-18 exerts a protective effect by inhibiting the excessive activation of complement.
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Objective To construct and express a small complement receptor type 1 (CR1) deriv-ative which contained two functional domains. Methods Total RNA was isolated from the peripheral blood mononuclear cell. The functional fragment Ⅰ of CR1 was amplified using the RT-PCR. The functional frag-ment Ⅱ was amplified with the plasmid of pET-32a-CR1-SCR15-18 as template which had been already con-structed in our laboratory. Then the chimeric gene that contained the two fragments was constructed with spli-cing overlap extention PCR. The chimeric gene was then inserted into the plasmid of pET-32a (+) and transformed into E. coli Rosetta(DE3). The inserted gene was verified by enzyme digestion and DNA se-quencing. After induced by IPTG, the expressed protein was analyzed by SDS-PAGE and Western blot. Then the fusion protein was purified by Ni-NTA affinity column and renatured through dialysis. The comple-ment inhibition activity was determined by CH50 method. Results The chimeric gene was successfully cloned into pET-32a(+). The result of SDS-PAGE and Western blot confirmed the expressed protein and showed that the molecule mass(Mr) of the expressed protein was 63×103. The purity of the recombinant protein was up to 92% after Ni-NTA column affinity chromatography. The bioactivity assay showed the fusion protein had a concentration-dependent complement inhibition activity within the concentration range of 0-200 μg/ml. Conclusion The two functional domains contained small CR1 derivative was successfully construc-ted and expressed in E. coli Rosetta. The fusion protein had a relative high bioactivity, providing a basis for further function experiment in vivo.
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Objective To investigate the residues of antibiotics such as quinolones in fishes from Guangzhou aquatic products market.Methods The residues of three kinds of quinolones(NFLX,CPFX and ENFX) in four kinds of fishes collected from five agriculture products markets in Guangzhou were determined by RP-HPLC.Results NFLX was detected in all samples,ranged from 2.19-192.8 ng/g with the maximum concentration of 192.8 ng/g,the detection rate of CPFX was 40% with the average concentration of 4.34 ng/g,ranged form 0-25.36 ng/g,the detection rate of ENFX was 45% with the average concentration of 6.83 ng/g.Three kinds of quinolones were found with high detection rate in Micropterus salmoides and Anguilla japonica,the average detection rates of NFLX,CPFX and ENFX in muscle tissues of Micropterus salmoides were 100%,40% and 100% respectively,that in Anguilla japonica were 100%,40% and 80% respectively.Low rate of ENFX was found in Monopterus albus and Ctenopharyngodon idellus muscle tissues,the average detection rates were 20% and 10%.Higher pharmaceuticals concentrations were detected in the liver than those in the muscle tissue.Conclusion The residues of quinolones in the muscle tissue of fish sold in Guangzhou is considered eligible based on the related standards.
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Objective To express the short consensus repeat 15-18 (SCR15-18) domain of human complement receptor 1 (CR1) in Pichia pastoris as a secreted protein in order to found a base for large-scale industry fermentation. Methods The gene fragment of CR1-SCR15-18 was amplified by PCR from plasmid pET32a-sCR1-SCR15-18. The obtained sequence was subcloned into Pichia pastoris secretory expression vector pPIC9. After identification the recombinant plasmid pPIC9-CR1-SCR15-18 was electrotransported into the yeast. Positive clones were identified using colony PCR. Positive recombinants were fermented in shake flaskes and induced with methanol. The recombinant proteins were identified with SDS-PAGE and Western blot analysis. After the recombinant protein was purified by Ni-NTA agarose metal chelate affinity chromatography, inhibiting complement hemolysis testing was used to detect the biological activity. Results Recombinant expression plasmid pPIC9-CR1-SCR15-18 was successfully constructed. SDS-PAGE and Western blot analysis revealed that the target gene was successfully expressed in the yeast and the recombinant protein was successful secreted in the culture supernatant. After purification, the protein inhibited complement hemolysis in vitro. Conclusion CR1-SCR15-18 is successful expressed in Pichia Pastoris with high activity of inhibiting complement hemolysis.
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Objective: To assay the content and stability of vitamin C(VC) in serum by a simple, specific method. Methods: Serum samples were extracted by metaphosphoric acid, and analyzed on a column C 18 of HPLC with DAD detector at 234 nm. Mobile phase was 100 mmol/L KH 2PO 4, pH3.00. The flow rate was 1 ml/min. Results: Under this condition, the retention time of VC was 5.76 min. The within run and between run reproducibilities tests were 0.42% and 2.11% respectively, and the recovery rate ranged from 82% to 88.5%. Stored at -70 ℃ and compared to that of its metaphosphoric acid extract, serum VC content was stable only for two days, and then decreased rapidly, whereas it did not decrease in metaphosphoric acid extract until the fifth day. Conclusion: A simple, sensitive and reliable method of HPLC with DAD detector is established for measuring VC in either serum or fruit, such as orange juice. VC should be determined immediately or should be extracted with metaphosphoric acid as soon as possible, then the extracts are kept at -70 ℃, and analysed within 5 d.
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Objective: To investigate the effects of vitamin B 1, C and orange juice on human lung cancer cell proliferation in vitro. Methods: Vitamin C degradation in culture medium was evaluated. The methods included: methy thiazolyl tetrazolium (MTT) assay, colony forming assay and 3H TdR incorporation test. The final concentrations of factors in medium were: orange juice (vitamin C 30,60,120 ?g/ml), vitamin C(30,60,120 ?g/ml), vitamin B 1 (0.01,0.1,1 ?g/ml). Results: (1) In MTT test, orange juice at each level had significant inhibitory effect on the growth of lung cancer cells (P