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Objective To prepare the folate receptor-targeted,doxorubicin and melanin-loaded multifunctional contrast media,and to observe its characteristics,targeting,imaging performance and cell toxicity on human MDA-MB-231 cell lines in vitro.Methods The folate receptor-targeted,doxorubicin and melanin-loaded multifunctional contrast media wasprepared using single emulsion method and freeze-drying and C3 Fs-inflation.Its size and drug loading efficiency were measured with Malvern instrument and UV-visible spectrophotometry.Its enhanced respectively ultrasound and photoacoustic imaging were observed in vitro model.The targeting performance of contrast media and its cell toxicity werechecked after co-incubated with human MDA-MB-231 cell lines.Results The average diameter of contrast media was (659.60±27.56)nm,Zeta potential was (-38.90±4.00)mV,and the drug loading capacity was 85.72 μg/mg.In vitro,the targeting experiment showed that a large number of folate receptor-targeted contrast media were aggregated and adhered firmly to the MDA-MB-231 cells.The folate receptor-targeted contrast media significantly inhibited the proliferation of human MDA-MB-231 cells.The ultrasound and photoacoustic imaging of folate receptor-targeted contrast media were significantly enhanced.Conclusion The folate receptor-targeted,doxorubicin and melanin-loaded multifunctional contrast media is a set of targeting treatment,enhancing ultrasound and photoacoustic imaging,having potential of perfect molecular probe for targeting imaging and treatment of human breast cancer.
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Objective To prepare multi-modality melanin-based lipid nanobubbles (MNBs) contrast agents,and to investigate their manifestations of ultrasound (US),photoacoustic (PA) and MRI in vitro.Methods MNBs and lipid nanobubbles (NBs) were prepared with the method of CHCl3-injection,freeze-drying and C3F8-inflation.The characteristics (shape,grain diameter,loading capacity of melanin and stability) of MNBs were obseved.US,PA and MRI were detected in vitro and the images were quantitatively analyzed.Results MNBs presented with homogenized size distribution,and transmission electron microscope (TEM) images demonstrated that the melanin particles were successfully entrapped into nanobubbles.Meanwhile,the loading capacity of melanin was 90.53 μg/mg.US signal increased in vitro with the rise of MNBs and NBs concentration.The ultrasonic manifestations of MNBs and NBs were the same,and the relative signal enhancement had no significant difference (P＞0.05).With the increased concentration of MNBs,the PA and MRI signals were stronger,but NBs showed no evident enhancement.Conclusion Multi-modality MNBs contrast agents are prepared successfully,which can enhance US,PA and MR imaging.
RESUMEN
Objective To prepare multi-modality melanin-based lipid nanobubbles (MNBs) contrast agents,and to investigate their manifestations of ultrasound (US),photoacoustic (PA) and MRI in vitro.Methods MNBs and lipid nanobubbles (NBs) were prepared with the method of CHCl3-injection,freeze-drying and C3F8-inflation.The characteristics (shape,grain diameter,loading capacity of melanin and stability) of MNBs were obseved.US,PA and MRI were detected in vitro and the images were quantitatively analyzed.Results MNBs presented with homogenized size distribution,and transmission electron microscope (TEM) images demonstrated that the melanin particles were successfully entrapped into nanobubbles.Meanwhile,the loading capacity of melanin was 90.53 μg/mg.US signal increased in vitro with the rise of MNBs and NBs concentration.The ultrasonic manifestations of MNBs and NBs were the same,and the relative signal enhancement had no significant difference (P＞0.05).With the increased concentration of MNBs,the PA and MRI signals were stronger,but NBs showed no evident enhancement.Conclusion Multi-modality MNBs contrast agents are prepared successfully,which can enhance US,PA and MR imaging.