Studies on amino acids in microfilariae of periodic Brugia malayi before and after cytotoxicity / 中华传染病杂志

Objective To study the mechanism of killing microfilariae in host by detecting the change of amino acids in Brugia malayi microfilariae. Methods The composition and contents of amino acid in microfilariae of periodic Brugia malayi before and after the effect of cytotoxicity were analyzed with automatic amino acid analyzer. Results The result showed that microfilariae contained 17 kinds of amino acids, but lacked Tryptophan. The total amount of amino acids of the microfilariae after cytotoxicity was lower than that before cytotoxicity( P

T CELL IMMUNE RESPONSE TO THE CIRCUMSPOROZOITE PROTEINOF PLASMODIUM FALCIPARUM, SOUTHERN CHINA ISOLATE FCC/HN AND ANTIGEN-INDUCED HL-2 AND IFN-γ Production ANALYSIS OF SPECIFIC / 中国人兽共患病学报

Inthis study, the DNA fragment encoding the regions Ⅰ to Ⅱ of CSP gene from Plasmodium falciparm isolate FCC1/HN was cloned into an. expression vector pcDNA3 contained cytomegalovius (CMV) and transformed into human Hela cell line. The expressed protein PfCSP (condidate vaccine) was used to immunize BALB/c mice by subcutaneous、 intravenous or intraperitoneal administration respectively. The splenocyte of BALB/c mice immumized with the condidate vaccine released significantly IL-2 and IFN-γ following stimulation with this vaccine. It is associated with increase of the splenic T lymphocyte proliferation stimulated by this vaccine and enhancement of NK cell killing activity in the former studies. These results suggested that the vaccine could stimulate T cell response and enhance the cell-mediated immunity.