PCR Amplification of 16S-23S rDNA Intergenic Spacer Regions of Bacteria in Trauma Infection by Universal Primers / 中华医院感染学杂志

OBJECTIVE To examine the feasibility of PCR amplification of 16S-23S rDNA intergenic spacer regions of bacteria in trauma infection by a pair of universal primers for gene diagnosis.METHODS The universal primers were designed at conserved regions of the 3' end of 16S rDNA and the 5' end of 23S rDNA.Bacterial genomic DNA from selected five commom bacteria in trauma infection were amplified by PCR.PCR products were examined using electrophoresis in agarose gel,and futher analyzed by sequencing.RESULTS The PCR products were similar to that we expected on the gel,which were confirmed by the results of sequencing and alignment.CONCLUSIONS Using the universal primers,16S-23S rDNA intergenic spacer regions of bacteria in trauma infection could be amplified by PCR,which lays a solid foundation for gene diagnosis in farther studies.

Distribution of neuropeptide Y-like immunoreactive fiber in rat temporomandibular joint / 华西口腔医学杂志

<p><b>OBJECTIVE</b>This investigation aimed at explore the total distribution of neuropeptide Y-like immunoreactive (NPY-LI) fibers and their changes post-trauma in rat temporomandibular joints (TMJs).</p><p><b>METHODS</b>Six groups of rats were killed individually before trauma, 1, 3, 7, 14 and 28 days after trauma. TMJs were extracted totally, and the avidin-biotin-peroxidase complex method and image analysis were employed to detect NPY-LI fibers in frozen sections of TMJs.</p><p><b>RESULTS</b>NPY-LI fibers were distributed extensively in TMJs, except the central disc band and bone, and they were mainly located around blood vessels, especially arteries. The densities of fibers in the six groups were 160.4 +/- 27.5, 95.8 +/- 16.4, 88.6 +/- 14.5, 114.3 +/- 17.0, 135.0 +/- 20.7, 158.6 +/- 19.5 (unit:mm2).</p><p><b>CONCLUSION</b>NPY-LI nerve fibers are distributed extensively in the periphery of blood vessels of TMJs and densities changed dynamically when TMJs were impacted. NPY may play an important role in pathologic change of TMJ by regulating local blood circulation.</p>

Serological Survey of Antibodies IgG and IgM to Toxoplasma among Population of Cangzhou City / 环境与健康杂志

Objective The aim of this research was to provide a basis for the development of preventive and curative measures against the toxoplasma infection by investigating the current infection of toxoplasma and its epidemic characteristics among the population of the Cangzhou city.Methods Antibodies IgG and IgM against toxoplasma in the sera were determined by ELISA during Jan.-May,2007.Results Six hundred and thirty-servn subjects were screened.The positive rate was 6.3% for total antibodies,5.2% for IgG and 1.1% for IgM.The infection rate was higher in the population aged 19-24 years and 49-60 years.The positive rate was higher in farmers living in countryside,those who were poor educated and who had bad habit of cooking food without separating knives and chopping boards to handle raw food and ready-to-eat food and washing their hands after cutting raw meat(P