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Objective To evaluate the expression of tumor necrosis factor related apoptosis-inducing ligand (TRAIL) and its receptor(TRAILR) in human hepatocellular carcinoma (HCC), and its therapeutic potential for human HCC. Methods Expressions of TRAIL and TRAILR were determined in and adjacent to carcinoma tissues, and normal liver tissues. The cytotoxic effects of TRAIL in combination with chemotherapeutic agents on HCC cell lines were detected. Results Expression of TRAIL in tumor adjacent tissue was higher than in cancer tissue. There were high expression of DR and low expression of DcR in HCC tissue in contrast to normal hepatic tissue(?~ 2=4.68,P
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Objective: To investigate the influence of esculentoside A（EsA） on production of IL-1 and TNF by rabbit synovial cells induced by LPS. Methods: levels of IL-1 and TNF in the supernatant of rabbit synovial cell were determined by examining proliferation of thymic cells and by bioassay L929 cells as target cells, respectively. Results: EsA in 5-40 μg/ml could significantly inhibit the production of IL-1 and TNF from rabbit synovial cells induced by LPS. Conclusion: EsA can inhibit the production of IL-1 and TNF from synovial cells. It suggests that EsA may play a role in improving the rheumatoid arthritis.
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Objective:To study the effects of several herbal medicines on SMMC-7721 liver cancer cells with Fourier transform infrared spectrometer(FTIR). Methods: FTIR was employed to determine the infrared spectra(IRs) of SMMC-7721 liver cancer cells cultivated for 20 h with the extracts of Spica prunellae, Herba houttuyniae, Radix bupleuri and Herba artemisiae scopariae. Cluster analysis of IRs was also performed. Results: IR spectral parameters such as band shape, intensity and frequency of the blank, control and herbal-extract-treated cells were compared. There existed obvious blue shift of ? s(PO 2 -), ? as (PO 2 -) bands, red shift of ? as (CH 3), ?(CH 2) bands on the herbal-extract-treated cells IRs. The decreasing ratio of ? as (CH 3) to ? s(CH 2) peak intensity and the increasing ratio of ? s(PO 2 -) to ?(N-H) peak area indicated the destructive effect of herbal extracts on the membrane structure of SMMU-7721 cells and inhibitory effect on the DNA replication respectively. Cluster analysis successfully discriminated the herbal-extract-treated cells from the blank cells and the liver-oriented medicines from the non-liver-oriented medicine. Conclusion: FTIR provides another fast and effective approach to analyze the changes of cells treated with Chinese herbal medicines, which may help to illuminate the functional mechanism of Chinese herbal medicines.
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Objective:To investigate the effect of esculentoside (EsA) on apoptosis of murine thymocyte. Methods:Using electronic microscope, DNA agarose electrophoresis and flow cytometry analysis,the effect of EsA on apoptosis of murine thymocyte was examined. Results:The result showed that apoptosis of activated thymocyte by ConA was markedly promoted by 2.5, 5, 10 ?g/ml EsA in murine thymocyte culture for 3 h, but the spontaneous apoptosis was not affected by EsA. Conclusion:The results suggest that EsA has the positive effect on apoptosis of activated murine thymocyte.