Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 11 de 11
Filtrar
1.
Journal of Peking University(Health Sciences) ; (6): 934-938, 2023.
Artículo en Chino | WPRIM | ID: wpr-1010152

RESUMEN

OBJECTIVE@#Agar dilution method (ADM) was used as the golden standard to evaluate the consistency of Epsilometer test (E-test) in detecting the sensitivity of Helicobacter pylori (H. pylori) to metronidazole.@*METHODS@#From August 2018 to July 2020, patients with H. pylori infection treated for the first time in Peking University Third Hospital for gastroscopy due to dyspepsia were included in this study. Gastric mucosas were taken from the patients with H. pylori infection. H. pylori culture was performed. Both the ADM and E-test were applied to the antibiotic susceptibility of H. pylori to metro-nidazole, and the consistency and correlation between the two methods were validated.@*RESULTS@#In the study, 105 clinical isolates of H. pylori were successfully cultured, and the minimum inhibitory concentration ≥ 8 mg/L was defined as drug resistance. Both ADM and the E-test showed high resistance rates to metronidazole, 64.8% and 62.9%, respectively. Among them, 66 drug-resistant strains were detected by ADM and E-test, and 37 were sensitive strains, so the consistency rate was 98.1%. Two strains were evaluated as drug resistance by ADM, but sensitive by the E-test, with a very major error rate of 1.9%. There was zero strain sensitive according to ADM but assessed as resistant by the E-test, so the major error rate was 0%. Taking ADM as the gold standard, the sensitivity of E-test in the detection of metronidazole susceptibility was 97.1% (95%CI: 0.888-0.995), and the specificity was 100% (95%CI: 0.883-1.000). Cohen's kappa analysis showed substantial agreement, and kappa coefficient was 0.959 (95%CI: 0.902-1.016, P < 0.001). Spearmans correlation analysis confirmed this correlation was significant (r=0.807, P < 0.001). The consistency evaluation of Bland-Altman method indicated that it was good, and there was no measured value outside the consistency interval. In this study, cost analysis, including materials and labor, showed a 32.2% higher cost per analyte for ADM as compared with the E-test (356.6 yuan vs. 269.8 yuan).@*CONCLUSION@#The susceptibility test of H. pylori to metronidazole by E-test presents better agreement with ADM. Because it is less expensive, less labor intensive, and more rapid, it is an easy and reliable method for H. pylori susceptibility testing.


Asunto(s)
Humanos , Metronidazol/uso terapéutico , Helicobacter pylori , Agar/uso terapéutico , Pruebas Antimicrobianas de Difusión por Disco , Pruebas de Sensibilidad Microbiana , Infecciones por Helicobacter/tratamiento farmacológico , Antibacterianos/uso terapéutico
2.
Journal of Experimental Hematology ; (6): 602-606, 2023.
Artículo en Chino | WPRIM | ID: wpr-982103

RESUMEN

Transplantation-associated thrombotic microangiopathy (TA-TMA) is one of the serious complications mostly occurring within 100 days after hematopoietic stem cell transplantation (HSCT). Risk factors of TA-TMA include genetic predispositions, GVHD, and infections. The pathophysiological mechanisms of TA-TMA start with endothelial injury caused by complement activation, which leads to microvascular thrombosis, and microvascular hemolysis, ultimately resulting in multi-organ dysfunction. In recent years, the development of complement inhibitors has markedly improved the prognosis of TA-TMA patients. This review will give an update on risk factors, clinical manifestations, diagnosis, and treatment of TA-TMA, so as to provide references for clinical practice.


Asunto(s)
Humanos , Microangiopatías Trombóticas/terapia , Pronóstico , Trombosis/etiología , Factores de Riesgo , Trasplante de Células Madre Hematopoyéticas/efectos adversos
3.
Acta Academiae Medicinae Sinicae ; (6): 497-503, 2020.
Artículo en Chino | WPRIM | ID: wpr-826334

RESUMEN

To develop an ideal surgical procedure for neobladder reconstruction in experimental porcine models. Six experimental female pigs weighting 28-33 kg underwent transplantation of autologous peritoneum for bladder reconstruction under general anesthesia.The flaps were used to reconstruct the orthotopic neobladder by suturing with the edges of the triangle and neck of the remnant bladder.The ureteral catheters were removed on the 5 postoperative day and the balloon catheter was removed on the 7 postoperative day.Voiding behaviour was monitored.The animals were euthanized at week 12 for routine pathology,immunohistochemistry,and electron microscopy. All the pigs survived after the surgery,and no postoperative complication such as peritonitis,intestinal obstruction,or urinary fistula was observed.All the peritoneum-ileum composite free valves survived after transplantation.Voiding behaviour was normal after catheter removal,and the urine was clear.At autopsy,reconstructed bladders were healthy.Pathological examination showed the neobladder had been covered by continuous urothelium while the peritoneum disappeared and showed no ileal mucosa regrowth and residual.Scanning electron microscope showed the transitional cells of neobladder were complete and orderly,and the urothelium around suture border was continuous and showed no malposition. Reconstruction of bladder by autologous peritoneum and ileal seromuscular flaps is an ideal approach in the experimental pigs as it can prevent regrowth of ileal epithelial cells and avoid the complications of conventional enterocystoplasty.Its clinical application deserves further investigations.


Asunto(s)
Animales , Femenino , Cistectomía , Íleon , Peritoneo , Complicaciones Posoperatorias , Colgajos Quirúrgicos , Porcinos , Neoplasias de la Vejiga Urinaria
4.
Chinese Medical Journal ; (24): 992-999, 2016.
Artículo en Inglés | WPRIM | ID: wpr-290139

RESUMEN

<p><b>OBJECTIVE</b>Helicobacter pylori (H. pylori) eradication remains a challenge with increasing antibiotic resistance. Hybrid therapy has attracted widespread attention because of initial report with good efficacy and safety. However, many issues on hybrid therapy are still unclear such as the eradication efficacy, safety, compliance, influencing factors, correlation with antibiotic resistance, and comparison with other regimens. Therefore, a comprehensive review on the evidence of hybrid therapy for H. pylori infection was conducted.</p><p><b>DATA SOURCES</b>The data used in this review were mainly from PubMed articles published in English up to September 30, 2015, searching by the terms of "Helicobacter pylori" or "H. pylori", and "hybrid".</p><p><b>STUDY SELECTION</b>Clinical research articles were selected mainly according to their level of relevance to this topic.</p><p><b>RESULTS</b>Totally, 1871 patients of 12 studies received hybrid therapy. The eradication rates were 77.6-97.4% in intention-to-treat and 82.6-99.1% in per-protocol analyses. Compliance was 93.3-100.0%, overall adverse effects rate was 14.5-67.5%, and discontinued medication rate due to adverse effects was 0-6.7%. H. pylori culture and sensitivity test were performed only in 13.3% patients. Pooled analysis showed that the eradication rates with dual clarithromycin and metronidazole susceptible, isolated metronidazole or clarithromycin resistance, and dual clarithromycin and metronidazole resistance were 98.5%, 97.6%, 92.9%, and 80.0%, respectively. Overall, the efficacy, compliance, and safety of hybrid therapy were similar with sequential or concomitant therapy. However, hybrid therapy might be superior to sequential therapy in Asians.</p><p><b>CONCLUSIONS</b>Hybrid therapy showed wide differences in the efficacy but consistently good compliance and safety across different regions. Dual clarithromycin and metronidazole resistance were the key factor to efficacy. Hybrid therapy was similar to sequential or concomitant therapy in the efficacy, safety, and compliance.</p>


Asunto(s)
Humanos , Claritromicina , Farmacorresistencia Bacteriana , Quimioterapia Combinada , Helicobacter pylori , Cumplimiento de la Medicación , Metronidazol , Inhibidores de la Bomba de Protones
5.
Chinese Medical Journal ; (24): 3101-3105, 2015.
Artículo en Inglés | WPRIM | ID: wpr-275560

RESUMEN

<p><b>OBJECTIVE</b>Although Helicobacter pylori (H. pylori) is considered as the main etiological factor for gastric cancer, the strategy of screening and treating the oncogenic bacterium is still controversial. The objective was to evaluate the status and progress of the cognition about the relationship between H. pylori infection and gastric cancer from a clinical aspect.</p><p><b>DATA SOURCES</b>The data used in this review were mainly from the PubMed articles published in English from 1984 to 2015.</p><p><b>STUDY SELECTION</b>Clinical research articles were selected mainly according to their level of relevance to this topic.</p><p><b>RESULTS</b>Gastric cancer is the fifth most common malignancy and the third leading cause of cancer deaths worldwide. The main etiological factor for gastric cancer is H. pylori infection. About 74.7-89.0% gastric cancer was related to H. pylori infection. Up to date, some regional gastric cancer prevention programs including the detection and treatment of H. pylori infection are under way. Current data obtained from the randomized controlled trials suggest that population-based H. pylori screening and treatment is feasible and cost-effective in preventing gastric cancer; however, a population-based H. pylori eradication campaign would potentially lead to bacterial resistance to the corresponding antibiotics, as well as a negative impact on the normal flora.</p><p><b>CONCLUSIONS</b>The important questions of feasibility, program costs, appropriate target groups for intervention, and the potential harm of mass therapy with antibiotics must first be answered before implementing any large-scale program.</p>


Asunto(s)
Humanos , Antibacterianos , Usos Terapéuticos , Análisis Costo-Beneficio , Infecciones por Helicobacter , Economía , Helicobacter pylori , Virulencia , Tamizaje Masivo , Economía , Neoplasias Gástricas , Microbiología
6.
Journal of Applied Clinical Pediatrics ; (24)2006.
Artículo en Chino | WPRIM | ID: wpr-639277

RESUMEN

Objective To comprehend the action mechanism of simvastatin in pulmonary hypertension(PH)when it induced by high pulmonary blood flow.Methods Abdominal aorta-inferior vena cava shunting was made in rats to establish animal model of PH induced by high pulmonary blood flow,simvastatin with dose of 2 mg/(kg?d)was used to interfere for 11 weeks.And then,pulmonary arterial pressure,apoptosis rate and proliferation rate of pulmonary vascular smooth muscle cell were determined.Results were compared with other groups.Results Simvastatin could cut down the pulmonary arterial pressure well,pulmonary arterial pressures of simvastatin group rats were lower than those of spliting groups obviously(Pa

7.
Chinese Medical Journal ; (24): 371-375, 2004.
Artículo en Inglés | WPRIM | ID: wpr-346667

RESUMEN

<p><b>BACKGROUND</b>We constructed a cDNA subtractive library of dermal papilla cells (DPCs) in anagen with suppression subtractive hybridization (SSH) technique and clone differentially expressed genes related to DPCs in anagen.</p><p><b>METHODS</b>Total mRNA was isolated from DPCs of anagen and telogen follicles. Moreover, single-strand (ss) and double-strand (ds) cDNAs were synthesized in turn using SMART PCR cDNA synthesis technology. ds cDNAs then were digested with Rsa I and divided into two groups, and ligated to the specific adaptor 1 and adaptor 2R, respectively. After cDNAs were hybridized with each other twice and underwent two rounds of nested PCR. PCR products were ligated with arms of T/A plasmid vectors to set up the subtractive library. Selected clones were demonstrated by reverse Northern blot and sequenced. The acquired sequence data were aligned against the Genbank nucleotide database.</p><p><b>RESULTS</b>cDNA subtractive library of DPCs in anagen follicles was set up successfully with high subtractive efficiency. Thirty-five genes were identified in this study with 22 known functional genes and 13 unknown functional genes.</p><p><b>CONCLUSIONS</b>All results confirm the effectiveness and sensitivity of SSH in detecting differentially expressed genes from a small amount of clinical samples. Information about such alterations in gene expression could be useful for elucidating the genetic events in hair follicle growth regulation.</p>


Asunto(s)
Adulto , Femenino , Humanos , Alopecia Areata , Genética , ADN Complementario , Biblioteca de Genes , Cabello , Folículo Piloso , Química , Hibridación de Ácido Nucleico , Reacción en Cadena de la Polimerasa
8.
Chinese Journal of Experimental and Clinical Virology ; (6): 77-80, 2003.
Artículo en Chino | WPRIM | ID: wpr-281849

RESUMEN

<p><b>BACKGROUND</b>To test susceptibility of human liver cell line Hep G2 to HCV in vitro.</p><p><b>METHODS</b>Hep G2 was cultivated with the serum from a chronic hepatitis C patient. After inoculation, plus and minus strand of HCV RNA, the expression of HCV NS3 antigen and the location of HCV RNA in cell and/or supernatant were examined by RT-PCR, immunohistochemistry and in situ hybridization, respectively.</p><p><b>RESULTS</b>HCV RNA could be detected from day 2 to day 40 post-inoculation in both cell and supernatant. HCV NS3 antigen could be expressed in infected cells and HCV RNA was mainly situated within cytoplasm.</p><p><b>CONCLUSIONS</b>The results suggested that HepG2 cell line was not only susceptible to HCV but also could support its replication in vitro.</p>


Asunto(s)
Humanos , Carcinoma Hepatocelular , Patología , Virología , Técnicas de Cocultivo , Hepacivirus , Genética , Fisiología , Hepatitis C Crónica , Virología , Neoplasias Hepáticas , Patología , Virología , ARN Viral , Genética , Células Tumorales Cultivadas , Proteínas no Estructurales Virales , Genética , Replicación Viral
9.
Journal of Third Military Medical University ; (24): 204-207, 2001.
Artículo en Chino | WPRIM | ID: wpr-736932

RESUMEN

Objective To study the similarities and differences on in vitro replication and expression of hepatitis C virus (HCV) between human fetal hepatocytes (HFH) and 7721 cell line. Methods Human fetal hepatocytes and a hepatoma cell line 7721 were incubated with a serum from hepatitis C patient. After incubation, the presence of HCV RNA, the expression of HCV NS3 antigens in cells and/or supernatant were examined by RT-PCR, in situ hybridization and immunohistochemistry, respectively. Results It was found that: ①The intracellular HCV RNA was first detected on d 2~3 post-incubation and then could be intermittently detected in cells and/or supernatant subsequently (HCV RNA could be detected in 7721 cells during a period of at least 66 days. In HFH, HCV RNA could be detected up to 25 days after incubation); ②HCV-NS3 antigen could be expressed in infected cells; ③Minus-strand RNA of HCV was mainly located within cytoplasm by in situ hybridization. Conclusion The results suggest that both the fetal hpatocytes and the hepatoma cell line 7721 are susceptible to HCV, and especially 7721 cell line can stably support HCV replication in vitro and may be used as the target cell for long-term cultures of HCV.

10.
Journal of Third Military Medical University ; (24): 204-207, 2001.
Artículo en Chino | WPRIM | ID: wpr-735464

RESUMEN

Objective To study the similarities and differences on in vitro replication and expression of hepatitis C virus (HCV) between human fetal hepatocytes (HFH) and 7721 cell line. Methods Human fetal hepatocytes and a hepatoma cell line 7721 were incubated with a serum from hepatitis C patient. After incubation, the presence of HCV RNA, the expression of HCV NS3 antigens in cells and/or supernatant were examined by RT-PCR, in situ hybridization and immunohistochemistry, respectively. Results It was found that: ①The intracellular HCV RNA was first detected on d 2~3 post-incubation and then could be intermittently detected in cells and/or supernatant subsequently (HCV RNA could be detected in 7721 cells during a period of at least 66 days. In HFH, HCV RNA could be detected up to 25 days after incubation); ②HCV-NS3 antigen could be expressed in infected cells; ③Minus-strand RNA of HCV was mainly located within cytoplasm by in situ hybridization. Conclusion The results suggest that both the fetal hpatocytes and the hepatoma cell line 7721 are susceptible to HCV, and especially 7721 cell line can stably support HCV replication in vitro and may be used as the target cell for long-term cultures of HCV.

11.
Chinese Journal of Dermatology ; (12)1994.
Artículo en Chino | WPRIM | ID: wpr-674183

RESUMEN

Objective To explore the feasibility of culturing dermal papillae cells (DPC) of hu- man hair in a serum-flee medium,and to observe the growth characteristics of these cells.Methods Cell culture flasks (plates) were pretreated with fibronectin,and DPC (2nd passage) were incubated with Williams E serum-flee medium supplemented with insulin-transferrin-selenite (ITS).Cells were observed by an inverted phase-contrast microscope.Proliferation of DPC was evaluated with 3-(4,5-dimethylthia- zol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay and by their growth curve.Results In a serum-free medium,2nd passage DPC adhered to the flask surface within two to four hours of incubation; two to three days later,confluence,of the cells was observed,without noticeable proliferation.Four days later,cell connection was interrupted,isolated cells or cell clusters were seen,and detachment of some cells from the flask surface was observed.One to two weeks later,most cells had died.After incubation with 4% bovine serum for ten hours,cell proliferation was observed surrounding the remaining viable cell colonies. DPC growth curve showed stagnant phase and slow growth phase;however,log growth phase was not ob- served.Conclusion DPC could be successfully cultured in serum-free medium.However,the culture con- dition needs to be further optimized.

SELECCIÓN DE REFERENCIAS
DETALLE DE LA BÚSQUEDA