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Chinese Journal of Applied Physiology ; (6): 573-576, 2009.
Artículo en Chino | WPRIM | ID: wpr-301558

RESUMEN

<p><b>AIM</b>To obtain Clenbuterol monoclonal antibodies.</p><p><b>METHODS</b>Clenbuterol complete antigen was prepared with diazotization method. BALB/c mice was immunized with subtractive immunization, Clenbuterol monoclonal antibody was prepared with rule hybridoma technique.</p><p><b>RESULTS</b>The mice obtained tolerance to BSA by subtractive immunization. The rate of the hybridoma cell with positive reaction which had obtained was 8.2%, and the specific clenbuterol monoclonal antibody was obtained at last.</p><p><b>CONCLUSION</b>Monoclonal antibodies to micromolecule contaminant be prepared by subtractive immunization, could decrease the workload in the bolting of monoclonal antibodies, and increase the chance to obtain the antibody of expected.</p>


Asunto(s)
Animales , Femenino , Masculino , Ratones , Anticuerpos Monoclonales , Alergia e Inmunología , Clenbuterol , Alergia e Inmunología , Hibridomas , Metabolismo , Inmunización , Métodos , Ratones Endogámicos BALB C , Albúmina Sérica Bovina , Alergia e Inmunología
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