RESUMEN
Objective To investigate the serum levels of miR-661 in type 2 diabetes(T2DM)patients and type 2 diabetes with microvascular complications(T2DMC),and to further evaluate its clinical auxiliary diagnosis significance.Methods The serum levels of miR-661 were examined in 60 T2DM patients,60 T2DMC patients and 60 healthy controls using quantitative real-time PCR (qRTPCR).The sera levels of biochemical parameters including fasting plasma glucose (FPG),cholesterol (TC),triglycerides (TG),low density lipoprotein (LDL-C),high density lipoprotein (HDL-C) and haemoglobinA1C (HbA1 c) were determined by biochemical analyzer.The ROC curve analysis,correlation analyses and logistic regression analyses were performed to evaluate the clinical auxiliary diagnosis value of serum miR-661,respectively.Results The qRT-PCR results showed that the concentrations of miR-661 was 118 (63.6,192.4) fmol/L in healthy controls,which were significant lower than those of T2DM patients [230.1 (83.6,426.2) fmol/L,(U =1 207,P ＜ 0.01)] and T2DMC patients [441.3 (212.9,1 021) fmol/L,(U =435,P ＜ 0.01)].ROC curve (AUCROc) of miR-661 was 0.665(95％CI:0.585 ～0.765,P ＜0.01)for T2DM patients and 0.879(95％CI:0.821 ～ 0.938,P＜0.01)for T2DMC patients.The AUCROc between T2DM and T2DMC patients was 0.694 (95 ％ CI:0.600 ～ 0.788,P ＜ 0.01).The correlation analyses revealed that the levels of miR-661 was positively correlated with concentrations of FPG(r =0.291,P ＜ 0.05)and LDL-C(r =0.149,P ＜ 0.05),and negatively correlated with concentrations of HDL-C (r =-0.243,P ＜ 0.01).Logistic regression analysis revealed that serum miR-661 was an independent risk factor in T2DM and T2DMC(P ＜ 0.01).Conclusion Serum miR-661 was potential biomarker and risk factor for T2DM and T2DMC.
RESUMEN
Objective To investigate the levels and clinical significance of serum miR-150 in children with primary nephrotic syndrome(NS).Methods Serum samples were collected from 78 NS children and 79 age-and sex-matched control children in Nanjing General Hospital,Jiangsu Province Hospital of TCM and Nanjing Children Hospital from March 2010 to May 2014.Quantitive Real-time PCR(qRT-PCR)assays were used to determine the concentrations of serum miR-150 in NS and control children.The other lipid and renal function parameters including serum TP,ALB,GLO,TC,TG,Urea,Cr,Uric acid and Uric protein were also assessed.Statistical analyzes were used to evaluate the clinical value of serum miR-150 for NS as well as to assess the clinical association between the levels of serum miR-150 and other clinical parameters.Results The ser-um levels of miR-150 were significantly elevated in NS children[101.4(21.29~336.6)fmol/L(F=3.658,P<0.001)]as compared with controls[34.11(5.53~134.2)fmol/L].ROC curve analysis showed that the area under the receiver operat-ing characteristic curve(AUCROC)was 0.892(95% CI=0.843~0.940).Spearman rank correlations analyzes showed that the levels of serum miR-150 were significantly negatively associated with GLO(r=-0.231,P=0.042)and TG(r=-0.233,P=0.040)in NS children.Multiple linear regression analyses showed that serum miR-150 was independently associ-ated with serum ALB levels(β=0.241,P=0.034;adjusted r2=0.046)after adjustment of other related factors.Further-more,multivariate logistic regression analysis showed that serum miR-150 an independent risk factor for NS after adjusting other factors including age and gender[OR=16.07(95% CI=5.35~48.28),P<0.001].Conclusion The serum levels of miR-150 were markedly elevated in NS children and closely associated with with impaired kidney function as well as lipid pa-rameters,and may have the potential as a novel auxiliary diagnosis marker for assessing the development of NS.