RESUMEN
AIM: To establish a RP HPLC method for the determination of 4′ O ? D glycosyl 5 O methylvisamminol in Jingfuzhiyang Granules (Herba Schizonepetae, Fructus kochiae, Radix Saposhnikoviae, Fructus Crataegi, etc.). METHODS: The sample was separated on Turnner Kromasil C 18 column (4.6mm?200mm,5?m) with mobile phase of methanol 0.05mol?L -1 potassium dihydrogen phosphate acetic acid isopropyl alcohol (85∶170∶4∶4,v/v). The detection wavelength was set at 254nm and the flow rate was 1.0mL?min -1 . The column temperature was 30℃. RESULTS: The calibration curve was linear in the range of 0.0606～0.606?g (r=0.9994). The average recovery was 92.2%. CONCLUSION: This method proves to be relatively simple and accurate, and available for the quality control of Jingfuzhiyang Granules