RESUMEN
OBJECTIVE: To model and optimize the reaction conditions of enzymatic esterification of cholesterol with diethenyl decanedioate in isooctane by response surface metodology (RSM).
RESUMEN
Objective: To synthesize cholesteryl vinyl hemi-sebacate from cholesterol and divinyl sebacate in non aqueous phase. Methods: TLC, MS, and NMR were used to identify the structure of production; the technological conditions of enzymatic esterification were determined through orthogonal test. Results: The best condition: isooctane 5 mL, reaction temperature 35°C, reaction time 24 h, Candida rugosa Lipase 10 mg/mL, molar ratio of cholesterol to divinyl sebacate 1:6. Conclusion: The highest esterification rate is above 95%.
Asunto(s)
Humanos , Masculino , Adulto Joven , Candida/clasificación , Candidiasis/microbiología , Infección Hospitalaria/microbiología , Fungemia/microbiología , Anfotericina B/uso terapéutico , Antifúngicos/uso terapéutico , Candidiasis/tratamiento farmacológico , Infección Hospitalaria/tratamiento farmacológico , Fungemia/tratamiento farmacológico , Adulto JovenRESUMEN
Objective To compare discrepancy between genotype and pheotype of th e clinical isolates of Candida rugosa. Methods The fung us-specific universal prim ers derived from the internal transcribed spacer (ITS) region of fungal rDNA wer e used for amplification. Genomic DNA purified from the thirteen clinical isolat es of non-C. Albicans was amplified by PCR. The purified PCR product was cloned into pBluescript Ⅱ KS(+) T vector and sequenced by Sanger's dideoxy chain terminatio n composition method. The two isolates were evaluated against CHROM Candida medi um and API 20C AUX. Results The two isolates of Candida rugosa were evaluated as Candida tropicalis by CHROM Candida medium and API 20C AUX. Conclusio ns Discrep ancy between genotype and phenotype of the two clinical isolates of Candida ru gosa was confirmed.