RESUMEN
Objective: To investigate the effects of Cannabis Fructus oil on the regulation of gut microecology in D-galactose-induced aging mice. Methods: The Kunming mice were divided into control group, model group, positive control group (Jin Shuangqi group), Cannabis Fructus oil (12.0, 6.0, 3.0 mL/kg) group with six males and six females in each group. In the control group, normal saline was injected subcutaneously into the back of the neck every day. The other five groups were injected subcutaneously with 1% D-galactose aqueous solution, and the volume was 10 mL/kg. After 1 h, the control group was given normal saline by intragastric administration. The other groups were intragastrically administered with different doses of Cannabis Fructus oil for 42 d, and the dosage volume was 20 mL/kg. After the end of the administration, the change in body weight was analyzed; The proximal intestinal tissue of the ileocecal area and the feces in the cecum and colon were retained. Gram staining was used for the detection of Gram-positive bacilli (G+b), Gram-negative bacilli (G-b), Gram-positive cocci (G+c) and Gram-negative cocci (G-c); The ileal mucosa changes were observed by HE staining; The pH value of the colon feces was determined by pH meter; And the content of short-chain fatty acids (SCFA) in feces was determined by gas chromatography. Results: The results showed that Cannabis Fructus oil increased the ratio of bacillus, reduced the ratio of cocci and the cecal coefficient, decreased the pH value of the colon, significantly improved the colon pathological changes of the model animals with unbroken membrane skin, regular glands and rich cup cells and fluff rich, increased the content of SCFAs in the intestine of mice, and significantly increased (P < 0.01) the content of acetic acid, propionic acid, butyric acid, isobutyric acid, and significantly reduced (P < 0.01) the content of isovaleric acid. Conclusion: It could conclude that Cannabis Fructus oil can up-regulate the ratio of D-galactose-induced mice intestinal bacteria structure, improve the intestinal microecology, so as to provide theoretical support for clinical application and product development of Cannabis Fructus oil.
RESUMEN
Objective To explore the characteristics of the interactions between traditional laxative medicine Cannabis Fructus and human gut microflora. Methods HPLC method was used to determine the content of the main unsaturated fatty acids linoleic acid and linolenic acid in Cannabis Fructus Decoction. At the same time, solid culture and liquid culture in vitro anaerobic culture method were combined with 16 S rRNA technology to analyze the interactions between Cannabis Fructus Decotion and human gut microflora. Moreover, the metabolits of linoleic acid and linolenic acid in Cannabis Fructus Decoction by human intestinal microflora were determined using HPLC method. At the same time, the possible conjugated linolenic acid and linoleic acid were determined. Results Cannabis Fructus Decoction promoted the growth of Proteobacteria significantly, which showed that Escherichia-shigella was significantly increased (P < 0.01), but the growth of Bacteroidetes was decreased (P < 0.01), and the content of unsaturated fatty acids linoleic acid and linolenic acid in Cannabis Fructus Decoction were reduced after being incubated with human intestinal bacteria, and the metabolites were conjugated linoleic acid and conjugated linolenic acid. Conclusion The interactions between Cannabis Fructus Decoction and human intestinal microflora are obvious. The Chinese medicine can change the structure of the gut microflora, and the gut microflora can metabolize the drug components. This analysis method partially restores the pharmacokinetics process of the oral administration drug in the human intestinal tract. It could provide a new insight of the mechanism research of Cannabis Fructus.
RESUMEN
Objective: To investigate the effects of the water extract of Cannabis Fructus on intestinal flora structure, short chain fatty acids (SCFAs) levels, and pathological changes of colonic inflammation, and explore its scientific connotation of laxative action. Methods: A total of 48 Wistar rats (SPF grade), sex in half, were randomly divided into control group, model group, Cannabis Fructus water extract group, and positive control group. The rats of model group were caused constipation and enteric flora disturbance by diphenoxylate. The bacterial counts in the fresh stool were tested by plate count method 28 days after administration, and the changes of intestinal flora structure in cecum was detected by PCR-DGGE method, the contents of SCFAs in cecum were determinated by GC method, and the coton pathological score of the rats were studied by HE staining method. Results: Compared with the blank group, the number of Escherichia coli and Bifidobacterium was increased but Bacillus acidi lactici was decreased significantly (P < 0.05, 0.01) in fresh stool of the model group. The ratio of Firmicutes bacteria to Bacteroides bacteria was increased, the number of Butyricicoccus and Cillobacterium was decreased, the concentration of acetic acid and butyric acid was increased significantly (P < 0.05, 0.01), and the pathology score of colon was increased significantly (P < 0.01). Compared with the model group, Cannabis Fructus water extract can adjust the ratio of Firmicutes bacteria to Bacteroides bacteria, increase the number of Cillobacterium, Butyricicoccus, Blautia, and Oscillibacter in cecum, decrease the number of Clostridium IV, etc., decrease the concentration of acetic acid and butyric acid (P < 0.05), reduce the pathology score, and improve the damage of intestinal structure (P < 0.05). Conclusion: Cannabis Fructus water extract can alleviate constipation and accelerate the recovery of colitis injury in model rats. The functional route of Cannabis Fructus water extract may relate to the disorder of intestinal flora balance adjustment and effect of the concentration of SCFAs so as to change the acidic environment in the intestinal tract.