Rapid chemome profiling of Cistanche salsa using DI-MS/MS~(ALL) / 中国中药杂志

The current study aims to rapidly and comprehensively profile the chemical composition of Cistanche salsa using direct infusion coupled with MS/MS~(ALL)(DI-MS/MS~(ALL)). The C. salsa extract was directly imported into electrospray ionization(ESI) source of quadrupole time-of-flight(Q-TOF) mass spectrometer with an infusion pump at a flow rate of 10 μL·min~(-1). Acquisition program was applied under negative ionization polarity to collect one MS~1 spectrum(m/z 50-1 200), followed by 1 150 MS~2 spectra with precursor isolation window(m/z 1) amongst mass range m/z 50-1 200. After each MS~2 spectrum was matched to its precursor ion, putative identification was conducted through matching mass spectral data with literature and database. A total of 31 components were identified from C. salsa, including 9 phenylethanoid glycosides, 2 iridoids, 4 saccharides, 9 organic acids, and 7 other compounds, similar to those from C. tubulosa and C. deserticola. In conclusion, DI-MS/MS~(ALL), a facile and reliable analytical tool, can be employed for qualitative analysis of chemical constituents in C. salsa. The research offers a promising strategy to achieve rapid chemome profiling of herbal medicine and provides an alternative source of Cistanches Herba.

Protective effect of glycosides of cistanche on immune function of ~(60) Co ?-ray irradiated mice / 中草药

Object To investigate the effect of cistanche glycosides (GCs) on immune functions and 30 d survival rate of 60 Co irradiated mice Methods Mice were given GCs both before and after 60 Co irradiation at different doses, and the changes of their immunological functions observed by routine immunopharmacologic methods Results When irradiated at doses of 2, 4, and 7 Gy with 60 Co, the humoral, cellular and non specific immunity, and 30 d survival rate were all decreased (P

Cistanche Salsa Tablets for Endemic Fluorosis:Observation of Clinical Efficacy / 中国药房

OBJECTIVE:To observe the clinical efficacy of Cistanche salsa tablets in treating endemic fluorosis. METHODS: A total of 137 cases were enrolled from endemic fluorosis disease-stricken area: 37 were assigned to receive Cistanche salsa tablets(1.2 g) po bid (trial group), and another 100 to receive Fenbid (300 mg) po bid as control for a treatment course of 4 weeks. The outcome measures included the total efficacy, time to take effect, adverse drug reaction and patients’ drug tolerance. RESULTS: In the trial group, the total effective rate was 86.49%, the time to take effect ranged from 7 to 15 days, and the ADR incidence rate was 35.14%, which was of significant difference as compared with control group.The patients in both groups had good drug tolerance. CONCLUSION: Cistanche salsa tablets are effective for endemic fluorosis and superior to Fenbid in efficacy.