Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 2 de 2
Filtrar
Añadir filtros








Intervalo de año
1.
Indian J Med Microbiol ; 2012 Jul-Sept; 30(3): 323-331
Artículo en Inglés | IMSEAR | ID: sea-143978

RESUMEN

Background: Culture filtrate proteins (CFPs) of Mycobacterium tuberculosis are potential vaccine candidates. Objective: The aim was to study the influence of iron levels on CFPs and assess the immuno-protective potential of defined antigenic fractions from high (8 μg Fe/mL) and low iron (0.02 μg Fe / mL) cultures of M. tuberculosis. Materials and Methods: The CFPs of M. tuberculosis from high (CFP-high) and low (CFP-low) iron conditions were first compared to identify iron-regulated proteins and then fractionated to obtain ten antigen pools (CF-Ags H1- H5 and L1-L5) that were used to assess the immune response of TB patients and normal healthy controls. Results: Iron limitation resulted in the up-regulation of two novel iron-regulated low-molecular-weight proteins Irp-1 (in CF-Ag L4) and Irp-2 (in CF-Ag L5) and repression of two ESAT proteins (identified with monoclonal antibody HYB 76.8). The median stimulation indices (SIs) against most of the CF-Ags were high in pulmonary TB patients. The CF-Ags L1 and L2 showed statistically significant SI (P values of 0.0027 and 0.0029 respectively); the % case recognition was high with these antigens as well as with L4 ( P = 0.0275). IFN-γ in response to these CF-Ags was significantly high in the endemic normals; maximal expression was seen with CF-Ag L5 (median value of 233 pg mL -1 ) that was higher than the corresponding H5 (140 pg mL -1 ) and H3 and L3 (205 and 206 pg mL -1 respectively). Conclusions: CF-Ags L5, H3 and L3 showed immuno-protective potential in this geographical location.


Asunto(s)
Adulto , Antígenos Bacterianos/biosíntesis , Antígenos Bacterianos/inmunología , Proteínas Bacterianas/biosíntesis , Proteínas Bacterianas/inmunología , Femenino , Humanos , Hierro/metabolismo , Masculino , Mycobacterium tuberculosis/inmunología , Mycobacterium tuberculosis/metabolismo , Linfocitos T/inmunología , Tuberculosis/inmunología , Tuberculosis/microbiología
2.
Braz. j. microbiol ; 41(2): 295-299, Apr.-June 2010. ilus
Artículo en Inglés | LILACS | ID: lil-545332

RESUMEN

Sample preparation for Two-dimensional gel electrophoresis (2DE) is tedious and not sufficient to provide a comparative profile of secreted proteins for various strains of M. tuberculosis. High lipid content in mycobacteria limits the use of common methods as it can hinder the 2DE run. This study highlights the significance of SDS-TCA procedure over common used methods for the preparation of sample from culture filtrate as well as other proteinaceous fluids.


Asunto(s)
Humanos , Cromatografía en Gel , Medios de Cultivo , Lípidos , Mycobacterium tuberculosis/metabolismo , Técnicas y Procedimientos Diagnósticos , Electroforesis en Gel Bidimensional , Métodos
SELECCIÓN DE REFERENCIAS
DETALLE DE LA BÚSQUEDA