Influência de um novo dispositivo acessório de fotopolimerização com fibra óptica e pino de fibra de vidro experimental nas propriedades mecânicas, físicoquímicas e adesão dos cimentos resinosos / Influence of a new light curing device with optical fiber and an experimental glass fiber post on the physicochemical, mechanical properties and adhesion of resin cement

O objetivo deste estudo foi avaliar in vitro a influência de um novo dispositivo fotopolimerização e pino de fibra de vidro nas propriedades mecânicas, físicoquímicas e adesão dos cimentos resinosos. Foram utilizados oitenta dentes bovinos, submetidos ao tratamento endodôntico, distribuídos em 5 grupos (n=16): CD (Pino fibra vidro e cimento dual); PF (Pino perfurado e cimento fotoativado); PD (Pino perfurado e cimento dual); POF (Pino perfurado iluminador de fibra óptica e cimento fotoativado); POD (Pino perfurado iluminador de fibra óptica e cimento dual). Os dentes foram preparados para colocação de um protótipo de pino em fibra de vidro que possui um canal interno de diâmetro regular e com conicidade progressiva. A perfuração permite a inserção da fibra óptica ao longo da extensão do pino a fim de possibilitar a ação da luz ao longo de todo o comprimento do conduto radicular. Após a cimentação, os espécimes foram seccionados perpendicularmente, e obtida 1 fatia de 2 mm de espessura do terço apical, médio e cervical. A avaliação mecânica foi realizada através do ensaio de push-out para determinação da resistência adesiva nos terços cervical, médio e apical seguido pela análise da fratura em estereomicroscópio. Os retentores intrarradiculares foram também submetidos ao teste de flexão de 3 pontos para análise do material preenchedor do pino de fibra (n=10). As análises físico - químicas foram realizadas através da determinação do grau de conversão dos cimentos (RAMAN) e análise em espectroscopia de energia dispersiva (EDS) dos monômeros presentes. A adesão foi analisada pela interface de cimentação pelo MEV e reconstrução 3D do novo sistema através do Micro-CT. Os dados obtidos foram analisados estatisticamente pelo ANOVA (um e dois fatores) e comparação múltipla de Tukey, (p<0,05). Os resultados de resistência adesiva evidenciaram que o terço apical obteve o maior valor de resistência adesiva em comparação ao terço médio (p<0,001), o grupo POD do terço apical foi estatisticamente significante em relação ao grupo CD do terço médio (p<0,001). Na analise individual de cada terço, não houve diferença entre os grupos experimentais e o controle (p>0,05). A falha adesiva entre cimento e dentina foi a mais predominante entre todos os grupos e terços. O pino de fibra de vidro (controle) obteve o maior valor de resistência à flexão (p<0,001), seguido do pino de fibra de vidro perfurado preenchido com cimento resinoso (p<0,001). Os maiores valores de GC foram alcançados pelo grupo POD com 82,3% (cervical) 69,9 % (médio) e 76,21% (apical) e o EDS comprovou a presença de componentes químicos adequados. A análise da adesão do novo pino de fibra de vidro comprova uma excelente adaptação no interior do canal radicular nas regiões cervical, médio e apical. Portanto o novo dispositivo com fibra óptica e pino de fibra de vidro experimental aumentaram as propriedades mecânicas, físico-químicas e adesão do cimento resinoso (AU)

The aim of this study was to evaluate in vitro the influence of a new light curing device with optical fiber and experimental glass fiber post on the physicochemical, mechanical, and adhesion properties of resin cements. Eighty bovine teeth were used, submitted to an endodontic treatment, distributed in 5 groups (n=16): CD (Glass fiber post and dual cement); PF (Perforated post and light-cured cement); PD (Perforated post and dual cement); POF (Fiber optic illuminating with a perforated post and lightcured cement); POD (Fiber optic illuminating with a perforated post and dual cement). The teeth were prepared for placement of a glass fiber post prototype, which has an internal canal of regular diameter and progressive taper. The internal perforation extension allows the insertion of the optical fiber along the entire length of the post in order to allow the action of light along the entire length of the root canal.The specimens were sectioned perpendicularly for the tests, and 1 slice approximately 2 mm thick was obtained from the apical, middle and cervical thirds. The mechanical evaluation was carried out through the push-out test to determine the adhesive bond strength, in the cervical, middle and apical thirds, followed by the fracture analysis under a stereomicroscope, the intraradicular post were also submitted to the 3-point bending test for material analys of the fiber post filler (n=10). The physicochemical analyzes were performed by determining the degree of conversion of the cements (RAMAN) of each sample and the analysis of energy dispersive spectroscopy (EDS) of the monomers present. Adhesion was analyzed by the cementation interface and 3D reconstruction of the new system through micro-CT, and finally, SEM analysis of the adhesive interface. The data obtained were analyzed for normality and statistically by ANOVA (one and two ways) and Tukey's multiple comparison (p<0.05). The adhesive bond strength results showed that the apical third had the highest value of adhesive strength compared to the middle third (p<0.001), and the POD group of the apical third was statistically significant in relation to the CD group of the middle third (p<0.001). In the individual analysis of each third, there was no difference between the experimental and control groups (p>0.05). Adhesive failure between cement and dentin was the most prevalent among all groups and thirds. The conventional post (control) had the highest flexural strength value (p<0.001), followed by the perforated fiberglass post filled with resin cement (p<0.001). The POD group achieved the highest GC values with 82.3% (cervical), 69.9% (medium), and 76.21% (apical) and EDS confirmed the presence of adequate chemical components. The analysis of the adhesion of the new fiberglass post proves an excellent adaptation inside the root canal in the cervical, middle and apical regions. Therefore, the new light curing device with optical fiber and experimental glass fiber post improved the resin cement's mechanical, phycochemical, and adhesion. (AU)

Effect of flowable composite or glass ionomer liners on shrinkage stress of a composite resin / Efecto de recubrimientos con resinas de baja viscosidad o ionómero vitreo sobre la tensión por contracción de una resina compuesta

ABSTRACT The aim of this study was to evaluate the effect of flowable composite or glass ionomer liners on the shrinkage stress of a restorative composite resin. Fifteen previously sandblasted metal boxes were attached to a universal mechanical testing machine (INSTRON 1011, Instron Corporation). Five of these boxes were filled with Filtek Z350 XT (FXT) Universal Restorative A2 (3M ESPE) (Group 1 or Control). Two further groups of 5 boxes were prepared by interposing a layer of Vitrebond Light Cure Glass Ionomer 3M ESPE (VGI) (Group 2 or G.I.) or Filtek Z350 XT Flowable Restorative A2 3M ESPE (FFR) (Group 3 or Flowable) between the box and the composite resin, completing with the same volume of composite as in Group 1. Upon activating lightcuring, the filled boxes mounted on the testing machine were videoed for 60 seconds (40 s photoactivation and 20 s postcuring), timed with a digital chronometer. Force values were recorded in newtons and converted into stress according to contact surface. Stress values were recorded every 10 s. Results were analyzed using repeated measures ANOVA. Mean and standard deviation in kPa (stress) recorded for each group were: Control group: 126.2 (30.8); G.I.: 48.4 (18); Flowable: 27.9 (19.5). Statistical analysis showed significant differences between the control group and the rest (p<0.01), with no significant difference between groups with glass ionomer liners and flowable resin liners (G.I. and Flowable). Under the experimental conditions of this study, it can be concluded that polymerization shrinkage stress can be reduced by the presence of a liner between the preparation and the restorative material.

RESUMEN El objetivo de este trabajo fue evaluar el efecto de la colocación de una capa de Composite flow o Ionómero vítreo sobre la tensión de contracción de un composite para restauración. Se utilizaron 15 cajas metálicas previamente arenadas y conectadas a la máquina universal para ensayos mecánicos (INSTRON 1011, Instron Corporation). Cinco de estas cajas (G1) se rellenaron con Filtek Z350 XT (FXT) Universal Restorative A2 3M ESPE. Al iniciar la activación de la unidad de curado se comenzaba a registrar con una cámara de video y un cronómetro digital desde el comienzo de la activación de la lámpara hasta 60 s después, registrando los valores post curado durante 20 s. Los valores de fuerza generados por la polimerización fueron registrados en newton de cada 10 s para los 15 ensayos. Los valores fueron convertidos en tensión de contracción según la superficie de contacto. Se realizaron además dos grupos de cajas (5 en cada una) en los cuales se colocaron una capa inicial de Vitrebond Light Cure Glass Ionomer 3M ESPE (VGI) (G2 o IV) y Filtek Z350 XT Flowable Restorative A2 3M ESPE (FFR) (G3 o Flow) y se completó con el mismo volumen de composite de las del GI. Los resultados obtenidos fueron analizados por medio de ANOVA para mediciones repetidas. La media y la desviación estándar en kPa (tensión o estrés de contracción) registrado para cada grupo fueron: Grupo control: 126.2 (30.8); IV: 48.4(18); Flow: 27.9(19.5). El análisis estadístico mostró diferencias estadística mente significativas entre el grupo control y el resto (p=0.00), pero no hubo diferencias significativas entre la presencia de Ionómero vítreo o Composite Flow (IV y Flow). En las condiciones experimentales de este trabajo puede concluirse que la tensión de contracción generada durante la polimerización puede ser disminuida por la presencia de algún material interpuesto entre la preparación y el composite restaurador.

Accuracy of irradiance and power of light-curing units measured with handheld or laboratory grade radiometers

Abstract This study measured and compared exitance irradiance and power of 4 commercial dental light-curing units (LCU) (Elipar S10, Elipar DeepCure-S, Corded VALO and Bluephase Style) using different types of radiometers. The devices used to analyze the LCU were classified as either handheld analog (Henry Schein, Spring, Demetron 100A, Demetron 100B and Demetron 200), handheld digital (Bluephase 1, Bluephase II, Coltolux, CureRite and Hilux), or laboratory instruments (Thermopile and Integrating Sphere). The laboratory instruments and the Bluephase II radiometer were also used to measure the LCU's power (mW). The LCU's were activated for 20 s (n=5). Data were analyzed using Kruskal-Wallis and Student-Newman-Keuls multiple comparison test (a=0.05). Among the LCU, the laboratory instruments presented different irradiance values, except for Corded VALO. The Coltolux and Hilux radiometers measured greater irradiance values compared to the laboratory instruments for the four LCUs tested. Within a given LCU, handheld analog units measured lower irradiance values, compared to handheld digital and laboratory instruments, except using the Spring radiometer for the Elipar S10 LCU. None of the handheld radiometers were able to measure similar irradiance values compared to laboratory instruments, except for Elipar S10 when comparing Bluephase 1 and Thermopile. Regarding power measurement, Bluephase II always presented the lowest values compared to the laboratory instruments. These findings suggest that the handheld radiometers utilized by practitioners (analog or digital) exhibit a wide range of irradiance values and may show lower outcomes compared to laboratory based instruments.

Resumo Esse estudo mensurou e comparou a irradiância e a energia de quatro fotopolimerizadores comerciais (Elipar S10, Elipar DeepCure-S, Corded VALO e Bluephase Style) utilizando diferentes tipos de radiômetros. Os dispositivos utilizados para analisar os fotopolimerizadores foram classificados em portáteis analógicos (Henry Schein, Spring, Demetron 100A, Demetron 100B e Demetron 200), portáteis digitais (Bluephase 1, Bluephase II, Coltolux, CureRite e Hilux), ou instrumentos laboratoriais (Thermopile e Integrating Sphere). Os instrumentos laboratoriais e o radiômetro Bluephase II também foram utilizados para medir a energia dos fotopolimerizadores (mW). Os fotopolimerizadores foram ativados por 20 s (n=5). Os dados foram analisados utilizando Kruskal-Wallis e teste de Student-Newman-Keuls (a=0,05). Dentre os fotopolimerizadores, os instrumentos laboratoriais apresentaram diferentes valores de irradiância, exceto para o Corded VALO. O Coltolux e o Hilux mensuraram uma irradiância maior comparado aos instrumentos de laboratório para os quatro fotopolimerizadores testados. Para o mesmo fotopolimerizador, os radiômetros analógicos portáteis mensuraram menores valores de irradiância quando comparados aos digitais portáteis ou aos instrumentos laboratoriais, exceto quando utilizado o Spring para o Elipar S10. Nenhuma diferença foi observada entre os instrumentos laboratoriais na irradiância do Corded VALO. Para a medição da energia, nenhuma diferença foi observada usando Thermopile e a Integrating Sphere para o Corded VALO. Para os outros fotopolimerizadores, cada instrumento indicou uma energia diferente. Esses achados sugerem que radiômetros portáteis utilizados pelos cirurgiões-dentistas (analógico ou digital) exibem uma ampla gama de valores de irradiância e podem mostrar medidas inferiores comparados aos instrumentos de laboratório.

Evaluation of intensity standards of tungsten-halogen and led curing units / Evaluación de estándares de intensidad de unidades de curado tugsteno-halógenas y leds

Current evidence indicates that the minimum light intensity of photo curing units required to polymerize in a reliable way a composite resin, in increments of 2mm, is 300mW/cm2. The recent introduction of new generations of composite resin materials for large volume increments, partially contrasts with ISO 4049 (2009), calling for the use of light intensity of 1,000mW/cm2. Therefore, it is considered relevant to carry out periodic measurements of the emission intensity of light-curing units of clinical use. The aim of this study was to test the intensity [mW/cm2] of a representative sample of tungsten-halogen and LED photopolymerization units used in private and public health service in different areas of the Valparaíso Region in Chile. This was achieved through the use of dental radiometers, without considering the variables of intensity modification over time (either spontaneously, by undesirable inherent characteristics of the device, or by programs of intensity modification in time), or the density of accumulated power needed. This in vitro diagnostic test, evaluated a sample of 507 units, 107 halogen and 400 LED, for a period of around one month, using two radiometers as measuring instruments. For LED units the Bluephase Meter® radiometer, from Ivoclar-VivadentTM was used, and for halogen units we used the Coltolux® from ColténeTM. As a result, 85% of the LED and halogen units achieved the minimum requirements of intensity needed for the polymerization of conventional dental biomaterials. However, only 25% from the tested units achieved a power density of 1,000mW/cm2.

Comparison of in vivo and in vitro models to evaluate pulp temperature rise during exposure to a Polywave® LED light curing unit

Abstract Objectives: To measure and compare in vivo and in vitro pulp temperature (PT) increase (ΔTEMP) over baseline, physiologic temperature using the same intact upper premolars exposed to the same Polywave® LED curing light. Methodology: After local Ethics Committee approval (#255,945), local anesthesia, rubber dam isolation, small occlusal preparations/minute pulp exposure (n=15) were performed in teeth requiring extraction for orthodontic reasons. A sterile probe of a temperature measurement system (Temperature Data Acquisition, Physitemp) was placed within the pulp chamber and the buccal surface was sequentially exposed to a LED LCU (Bluephase 20i, Ivoclar Vivadent) using the following exposure modes: 10-s low or high, 5-s Turbo, and 60-s high. Afterwards, the teeth were extracted and K-type thermocouples were placed within the pulp chamber through the original access. The teeth were attached to an assembly simulating the in vivo environment, being similarly exposed while real-time temperature (°C) was recorded. ΔTEMP values and time for temperature to reach maximum (ΔTIME) were subjected to two-way ANOVA and Bonferroni's post-hoc tests (pre-set alpha 0.05). Results: Higher ΔTEMP was observed in vitro than in vivo. No significant difference in ΔTIME was observed between test conditions. A significant, positive relationship was observed between radiant exposure and ΔTEMP for both conditions (in vivo: r2=0.917; p<0.001; in vitro: r2=0.919; p<0.001). Conclusion: Although the in vitro model overestimated in vivo PT increase, in vitro PT rise was close to in vivo values for clinically relevant exposure modes.

Irradiance and radiant exposures delivered by led light-curing units used by a left and right-handed operator

Abstract The combination of the restoration location, the hand preference of the operator using the light-curing unit (LCU), and the design of the LCU all can have an impact on the amount of the light delivered to the restoration. To evaluate the effect of left-handed or right-handed users, the position of the operator (dentist or assistant), and the LCU design on the irradiance, radiant exposure and emission spectrum delivered to the same posterior tooth. Two light emitting diode (LED) LCUs were tested: an angulated monowave LCU Radii-Cal (SDI, Victoria, Australia) and a straight aligned multi-peak LCU Valo Cordless (Ultradent, South Jordan, UT, USA). The irradiance values (mW/cm2), radiant exposure (J/cm2) and emission spectrum were measured using a sensor in maxillary left second molar tooth. The irradiance and radiant exposure were analyzed using three-way ANOVA followed by Tukey test (a=0.05). The emission spectra (nm) were analyzed descriptively. The interaction between LCU design, operator position, and hand preference significantly influenced the irradiance and radiant exposure (P<0.001). In all cases, Valo delivered significantly higher irradiance than Radii-Cal. The handedness and the operator position affected the irradiance and radiant exposure delivered from Valo. Operator position and access affect the irradiance and radiant exposure delivered to the maxillary left second molar. The irradiance and radiant exposure can be greater when a right-hand operator is positioned on the right side of the chair and a left-hand operator is positioned on the left side of the chair. This may result in better resin composite polymerization.

Resumo A combinação da localização da restauração, a preferência de mão do operador ao utilizar aparelhos fotopolimerizadores (AFP) com luz emitida por diodo (LED) e o formato do AFP podem afetar a quantidade de luz fornecida à restauração. O objetivo foi avaliar o efeito de operadores canhotos e destros, a posição do operador (dentista ou auxiliar), e o formato do AFP na irradiância, energia radiante e espectro de luz entregue ao mesmo dente posterior. Dois AFP foram testados: um com formato angulado, onda única Radii-Cal (SDI, Victoria, Australia) e um formato reto multi-pico Valo Cordless (Ultradent, South Jordan, UT, USA). Os valores de irradiância (mW/cm²), energia radiante (J/cm²) e espectro de luz foram medidos utilizando um sensor no segundo molar superior esquerdo. A irradiância e energia radiante foram analisados utilizando ANOVA 3 fatores seguido por teste de Tukey (a=0.05). O espectro de luz (nm) foi analisado de forma descritiva. A interação entre o formato do AFP, posição do operador e preferência de mão foram significativamente influentes na irradiância e energia radiante (P<0.001). Em todos os casos, Valo teve irradiância significativamente maior que Radii-Cal. A mão dominante e a posição do operador afetaram a irradiância e energia radiante com o Valo. Posição do operador e acesso afetou a irradiância e exposição radiante entregue ao segundo molar superior esquerdo. A irradiância e exposição radiante teve melhores resultados quando AFP foi utilizado com a mão direita pelo operador posicionado na cadeira do lado direito e mão esquerda do operador posicionado do lado esquerdo da cadeira. Estes resultados podem levar a uma melhor polimerização da resina composta.

Effects of radiant exposure values using second and third generation light curing units on the degree of conversion of a lucirin-based resin composite

Abstract Alternative photoinitiators with different absorption wavelengths have been used in resin composites (RCs), so it is crucial to evaluate the effectiveness of light-curing units (LCUs) on these products. Objective Using Fourier transform infrared analysis (FTIR) in vitro, the effects of varying radiant exposure (RE) values generated by second and third generation LED LCUs on the degree of conversion (DC) and maximum rate of polymerization (Rpmax) of an experimental Lucirin TPO-based RC were evaluated. Material and Methods 1 mm or 2 mm thick silicon molds were positioned on a horizontal attenuated total reflectance (ATR) unit attached to an infrared spectroscope. The RC was inserted into the molds and exposed to varying REs (18, 36 and 56 J/cm2) using second (Radii Plus, SDI) and third generation LED LCUs (Bluephase G2/Ivoclar Vivadent) or a quartz tungsten based LCU (Optilux 501/SDS Kerr). FTIR spectra (n=7) were recorded for 10 min (1 spectrum/s, 16 scans/spectrum, resolution 4 cm-1) immediately after their application to the ATR. The DC was calculated using standard techniques for observing changes in aliphatic to aromatic peak ratios both prior to, and 10 min after curing, as well as during each 1 second interval. DC and Rpmax data were analyzed using 3-way ANOVA and Tukey’s post-hoc test (p=0.05). Results No significant difference in DC or Rpmax was observed between the 1 mm or 2 mm thick specimens when RE values were delivered by Optilux 501 or when the 1 mm thick composites were exposed to light emitted by Bluephase G2, which in turn promoted a lower DC when 18 J/cm2 (13 s) were delivered to the 2 mm thick specimens. Radii Plus promoted DC and Rpmax values close to zero under most conditions, while the delivery of 56 J/cm2 (40 s) resulted in low DC values. Conclusions The third generation LCU provided an optimal polymerization of Lucirin TPO-based RC under most tested conditions, whereas the second generation LED-curing unit was useless regardless of the RE.

Evaluation of phenyl-propanedione on yellowing and chemical-mechanical properties of experimental dental resin-based materials

ABSTRACT Objective To evaluate the influence of phenyl-propanedione on yellowing and chemical-mechanical properties of experimental resin-based materials photoactivated using different light curing units (LCUs). Material and Methods Experimental resin-based materials with the same organic matrix (60:40 wt% BisGMA:TEGDMA) were mechanically blended using a centrifugal mixing device. To this blend, different photoinitiator systems were added in equimolar concentrations with aliphatic amine doubled by wt%: 0.4 wt% CQ; 0.38 wt% PPD; or 0.2 wt% CQ and 0.19 wt% PPD. The degree of conversion (DC), flexural strength (FS), Young’s modulus (YM), Knoop hardness (KNH), crosslinking density (CLD), and yellowing (Y) were evaluated (n=10). All samples were light cured with the following LCUs: a halogen lamp (XL 2500), a monowave LED (Radii), or a polywave LED (Valo) with 16 J/cm2. The results were analysed by two-way ANOVA and Tukey’s test (α=0.05). Results No statistical differences were found between the different photoinitiator systems to KNH, CLS, FS, and YM properties (p≥0.05). PPD/CQ association showed the higher DC values compared with CQ and PPD isolated systems when photoactivated by a polywave LED (p≤0.05). Y values were highest for the CQ compared with the PPD systems (p≤0.05). Conclusion PPD isolated system promoted similar chemical and mechanical properties and less yellowing compared with the CQ isolated system, regardless of the LCU used.

Carga microbiana de las lámparas de fotocurado en el uso y desuso de las barreras adhesivas de protección

Se realizó un estudio acerca del análisis de la carga microbiana en las lámparas de fotocurado en cuanto al uso y desuso de barreras adhesivas de protección, con el fin de que los resultados sirvan para hacer conciencia sobre el uso de las barreras adhesivas de protección, para resguardar tanto al profesional como a su ambiente de trabajo, y a los pacientes, evitando además la contaminación cruzada entre ellos, como con el medio oral y los demás instrumentos. Para la investigación se tomó una muestra de 47 lámparas, 24 con barrera y 23 sin barrera, para así determinar la cantidad de unidades formadoras de colonias por superficie, y además la presencia o ausencia de Escherichia Coli como indicador de microorganismo patógeno.

It has been made a study of the analysis of the microbial load in dental curing lights in the use and disuse of adhesive protective barriers, with the goal that the results serve to raise awareness on use of adhesive protective barriers to protect the professional, their work environment and patients, while avoiding cross-contamination between them, as with the oral environment and other instruments. For this research the sample were 47 lamps, 24 with barrier and 23 without barrier, to determine the number of colony forming units per surface, and also the presence or absence of Escherichia coli as pathogen indicator.

LED and Halogen Light Transmission through a CAD/CAM Lithium Disilicate Glass-Ceramic

The effect of thickness, shade and translucency of CAD/CAM lithium disilicate glass-ceramic on light transmission of light-emitting diode (LED) and quartz-tungsten-halogen units (QTH) were evaluated. Ceramic IPS e.max CAD shades A1, A2, A3, A3.5, high (HT) and low (LT) translucency were cut (1, 2, 3, 4 and 5 mm). Light sources emission spectra were determined. Light intensity incident and transmitted through each ceramic sample was measured to determine light transmission percentage (TP). Statistical analysis used a linear regression model. There was significant interaction between light source and ceramic translucency (p=0.008) and strong negative correlation (R=-0.845, p<0.001) between ceramic thickness and TP. Increasing one unit in thickness led to 3.17 reduction in TP. There was no significant difference in TP (p=0.124) between shades A1 (ß1=0) and A2 (ß1=-0.45) but significant reduction occurred for A3 (ß1=-0.83) and A3.5 (ß1=-2.18). The interaction QTH/HT provided higher TP (ß1=0) than LED/HT (ß1=-2.92), QTH/LT (ß1=-3.75) and LED/LT (ß1=-5.58). Light transmission was more effective using halogen source and high-translucency ceramics, decreased as the ceramic thickness increased and was higher for the lighter shades, A1 and A2. From the regression model (R2=0.85), an equation was obtained to estimate TP value using each variable ß1 found. A maximum TP of 25% for QTH and 20% for LED was found, suggesting that ceramic light attenuation could compromise light cured and dual cure resin cements polymerization.

Resumo Avaliou-se o efeito da espessura, cor e translucidez de uma cerâmica vítrea a base de dissilicato de lítio para CAD / CAM sobre a transmissão da luz de unidades de diodos emissores de luz (LED) e de quartzo-tungstênio-halogênio (QTH). Cerâmica IPS e.max CAD nas cores A1, A2, A3, A3.5 de translucidez alta (HT) e baixa (LT) foram cortadas (1, 2, 3, 4, 5 mm). Os espectros de emissão das fontes de luz foram determinados. A intensidade da luz incidente e transmitida através de cada espécime de cerâmica foi medida para determinar a percentagem de transmissão de luz (TP). Um modelo de regressão linear foi utilizado para a análise estatística. Houve interação significativa entre a fonte de luz e translucidez cerâmica (p = 0.008) e forte correlação negativa (r = -0.845, p <0.001) entre a espessura da cerâmica e TP. O aumento da espessura em uma unidade levou a uma redução média de 3.17 em TP. Não houve diferença significativa em TP (p = 0.124) entre as cores A1 (ß1 = 0) e A2 (ß1 = -0.45), mas ocorreu redução significativa para as cores A3 (ß1 = -0.83) e A3.5 (ß1 = -2.18). A interação QTH/HT proporcionou maior TP (ß1 = 0) do que LED/HT (ß1 = -2.92), QTH/LT (ß1 = -3.75) e LED/LT (ß1 = -5.58). A transmissão de luz foi mais eficaz utilizando QTH e cerâmica de alta translucidez, diminuiu à medida que a espessura de cerâmica aumentou, e foi maior para as cores A1 e A2. A partir do modelo de regressão (R2 = 0.85), obteve-se uma equação para estimar o valor de TP utilizando os valores de ß1 encontrado. Foi observada TP máxima de 25% para QTH e 20% para LED, sugerindo que a atenuação promovida pela cerâmica pode comprometer a ativação de um cimento resinoso fotoativado e de ativação dupla.

Thermal analysis of bulk filled composite resin polymerization using various light curing modes according to the curing depth and approximation to the cavity wall

OBJECTIVE: The purpose of this study was to investigate the polymerization temperature of a bulk filled composite resin light-activated with various light curing modes using infrared thermography according to the curing depth and approximation to the cavity wall. MATERIAL AND METHODS: Composite resin (AeliteFlo, Bisco, Schaumburg, IL, USA) was inserted into a Class II cavity prepared in the Teflon blocks and was cured with a LED light curing unit (Dr's Light, GoodDoctors Co., Seoul, Korea) using various light curing modes for 20 s. Polymerization temperature was measured with an infrared thermographic camera (Thermovision 900 SW/TE, Agema Infra-red Systems AB, Danderyd, Sweden) for 40 s at measurement spots adjacent to the cavity wall and in the middle of the cavity from the surface to a 4 mm depth. Data were analyzed according to the light curing modes with one-way ANOVA, and according to curing depth and approximation to the cavity wall with two-way ANOVA. RESULTS: The peak polymerization temperature of the composite resin was not affected by the light curing modes. According to the curing depth, the peak polymerization temperature at the depth of 1 mm to 3 mm was significantly higher than that at the depth of 4 mm, and on the surface. The peak polymerization temperature of the spots in the middle of the cavity was higher than that measured in spots adjacent to the cavity wall. CONCLUSION: In the photopolymerization of the composite resin, the temperature was higher in the middle of the cavity compared to the outer surface or at the internal walls of the prepared cavity. .

Phototoxic effect of blue light on the planktonic and biofilm state of anaerobic periodontal pathogens

PURPOSE: The purpose of this study was to compare the phototoxic effects of blue light exposure on periodontal pathogens in both planktonic and biofilm cultures. METHODS: Strains of Aggregatibacter actinomycetemcomitans, Fusobacterium nucleatum, and Porphyromonas gingivalis, in planktonic or biofilm states, were exposed to visible light at wavelengths of 400.520 nm. A quartz-tungsten-halogen lamp at a power density of 500 mW/cm2 was used for the light source. Each sample was exposed to 15, 30, 60, 90, or 120 seconds of each bacterial strain in the planktonic or biofilm state. Confocal scanning laser microscopy (CSLM) was used to observe the distribution of live/dead bacterial cells in biofilms. After light exposure, the bacterial killing rates were calculated from colony forming unit (CFU) counts. RESULTS: CLSM images that were obtained from biofilms showed a mixture of dead and live bacterial cells extending to a depth of 30-45 microm. Obvious differences in the live-to-dead bacterial cell ratio were found in P. gingivalis biofilm according to light exposure time. In the planktonic state, almost all bacteria were killed with 60 seconds of light exposure to F. nucleatum (99.1%) and with 15 seconds to P. gingivalis (100%). In the biofilm state, however, only the CFU of P. gingivalis demonstrated a decreasing tendency with increasing light exposure time, and there was a lower efficacy of phototoxicity to P. gingivalis as biofilm than in the planktonic state. CONCLUSIONS: Blue light exposure using a dental halogen curing unit is effective in reducing periodontal pathogens in the planktonic state. It is recommended that an adjunctive exogenous photosensitizer be used and that pathogens be exposed to visible light for clinical antimicrobial periodontal therapy.

Effect of a multi-layer infection control barrier on the micro-hardness of a composite resin

OBJECTIVE: The aim of this study was to evaluate the effect of multiple layers of an infection control barrier on the micro-hardness of a composite resin. MATERIAL AND METHODS: One, two, four, and eight layers of an infection control barrier were used to cover the light guides of a high-power light emitting diode (LeD) light curing unit (LCU) and a low-power halogen LCU. The composite specimens were photopolymerized with the LCUs and the barriers, and the micro-hardness of the upper and lower surfaces was measured (n=10). The hardness ratio was calculated by dividing the bottom surface hardness of the experimental groups by the irradiated surface hardness of the control groups. The data was analyzed by two-way ANOVA and Tukey's HSD test. RESULTS: The micro-hardness of the composite specimens photopolymerized with the LED LCU decreased significantly in the four- and eight-layer groups of the upper surface and in the two-, four-, and eight-layer groups of the lower surface. The hardness ratio of the composite specimens was <80% in the eight-layer group. The micro-hardness of the composite specimens photopolymerized with the halogen LCU decreased significantly in the eight-layer group of the upper surface and in the two-, four-, and eight-layer groups of the lower surface. However, the hardness ratios of all the composite specimens photopolymerized with barriers were <80%. CONCLUSIONS: The two-layer infection control barrier could be used on high-power LCUs without decreasing the surface hardness of the composite resin. However, when using an infection control barrier on the low-power LCUs, attention should be paid so as not to sacrifice the polymerization efficiency.

Effect of light-curing units on gap formation and microleakage of class II composite restorations

Aim: This in vitro study evaluated gap width formation and marginal microleakage in Class IIcomposite restorations light-cured with three different light-curing units. Methods: Standardizedcavities in the proximal surfaces of 36 human third molars were made with margins located belowthe cementoenamel junction. Cavities were restored with Filtek P60 (3M ESPE), inserted with aphotocondenser tip and light-cured with three different methods: GI - Optilux401 (halogen); GII –ColtoluxLED (LED) and GIII –UltraLumeLED5 (LED). After finishing the restorations, teeth weresubjected to a thermal cycling regimen of 500 cycles (5oC ± 2oC and 55oC ± 2oC), totalizing 500cycles. Thereafter, the teeth were sectioned in a buccolingual direction and in the center of therestorations. Half of the specimens (18) were used to evaluate marginal microleakage, by measuringof dye penetration in cross-sectioned specimens, and the other half was used to analyzed the gapformation width by SEM observations (1000X). Data were submitted to Kruskal-Wallis (α=0.05).Results: The mean values of gap width (μm) were: GI 3.28±3.34; GII 1.48±1.89 and GIII3.11±3.45, and microleakage was not affected by the light-curing units. Conclusions: Therewere no differences between the light-curing methods in gap formation and marginal microleakage.

Effect of reduced exposure times on the cytotoxicity of resin luting cements cured by high-power led

OBJECTIVE: Applications of resin luting agents and high-power light-emitting diodes (LED) light-curing units (LCUs) have increased considerably over the last few years. However, it is not clear whether the effect of reduced exposure time on cytotoxicity of such products have adequate biocompatibility to meet clinical success. This study aimed at assessing the effect of reduced curing time of five resin luting cements (RLCs) polymerized by high-power LED curing unit on the viability of a cell of L-929 fibroblast cells. MATERIAL AND METHODS: Disc-shaped samples were prepared in polytetrafluoroethylene moulds with cylindrical cavities. The samples were irradiated from the top through the ceramic discs and acetate strips using LED LCU for 20 s (50 percent of the manufacturer's recommended exposure time) and 40 s (100 percent exposure time). After curing, the samples were transferred into a culture medium for 24 h. The eluates were obtained and pipetted onto L-929 fibroblast cultures (3x10(4) per well) and incubated for evaluating after 24 h. Measurements were performed by dimethylthiazol diphenyltetrazolium assay. Statistical significance was determined by two-way ANOVA and two independent samples were compared by t-test. RESULTS: Results showed that eluates of most of the materials polymerized for 20 s (except Rely X Unicem and Illusion) reduced to a higher extent cell viability compared to samples of the same materials polymerized for 40 s. Illusion exhibited the least cytotoxicity for 20 s exposure time compared to the control (culture without samples) followed by Rely X Unicem and Rely X ARC (90.81 percent, 88.90 percent, and 83.11 percent, respectively). For Rely X ARC, Duolink and Lute-It 40 s exposure time was better (t=-1.262 p=0,276; t=-9.399 p=0.001; and t=-20.418 p<0.001, respectively). CONCLUSION: The results of this study suggest that reduction of curing time significantly enhances the cytotoxicity of the studied resin cement materials, therefore compromising their clinical performance.

Effect of different light-curing devices and aging procedures on composite knoop microhardness

The aim of this study was to evaluate the effect of light-curing devices (Halogen/HAL, Light Emitting Diodes/LED, Argon Laser/LAS and Plasma Arc/PAC) and aging procedures (Mechanical Cycling/MC, Thermal Cycling/TC, Storage/S, MC+TC and MC+TC+S) on the micro-hardness of bottom/B and top/T surfaces of 2-mm-high composite resin cylinders. The Knoop microhardness test (25 g, 20 s) on both B and T was performed before and after each aging procedure. For B and T, before aging procedures, PAC showed reduced polymerization effectiveness when compared with HAL. In the T, after TC, PAC and LAS had also showed reduced polymerization effectiveness when compared to HAL and LED. For all light-curing devices, MC+TC+S and S affected the Knoop microhardness values. In the B, no difference could be observed among the aging procedures for PAC. From all light-curing units, PAC may have rendered composites of reduced quality and the storage aging procedures were the most harmful to the polymer hardness.