RESUMEN
Objective To investigate the chemical components of gorgonian Menella kanisa collected from the South China Sea. Methods The Et2O extract of gorgonian Menella kanisa was purified by repeated column chromatography on silica gel, Sephadex LH-20, and reversed-phase high-performance liquid chromatography (RP-HPLC). The structures of the obtained compoundswere determined on the basis of spectroscopic analysis and compared with the reported data. Results and Conclusion Five 5α, 8α-epidioxy sterolswere isolated from gorgonian Menella kanisa collected from the South China Sea, and their structures were determined as: 5α, 8α-epidioxy-cholest-6-en-3/β-ol (1), (22E)-5α, 8α-epidioxy-cholesta-6, 22-dien-3/β-ol (2), (22E, 2iR)-5α, 8α-epidioxy-24-methyl-cholesta-6, 22-dien-β-ol (3), 5α, 8α-epidioxy-24-methyl-cholesta-6, 24 (28)-dien-β-ol (4), (24E)-5α, 8α-epidioxy-24-ethyl-cholesta-6, 24(28)-dien-β-ol (5). All the five epidioxy sterols have been separated from the title Menella kanisa for the first time.
RESUMEN
Objective: To investigate the bioactive chemical constituents of gorgonian Muriceopsis flavida collected from the South China Sea. Methods: The Et2O extract of the sample was purified by repeated column chromatographies on silica gel, Sephadex LH-20, and RP-HPLC. The structures of the obtained compounds were elucidated based on detailed spectroscopic analysis using mass spectrometry (MS) and nuclear magnetic resonance (NMR). The in viteo antimicrobial activities of the identified compounds were assessed by an agar diffusion test. Results: Five 5α, 8α-epidioxy sterols were isolated from the Et2O extract (1-5), and their structures were determined as: (22E, 24S)-5α, 8α-epidioxy-24-methyl-cholesta-6, 22-dien-3β-ol (1), (22E,24R)-5α, 8α-epidioxy-24-methyl-cholesta-6, 22-dien-3β-ol (2), (24R)-5α, 8α-epdioxr24-ethyl-cholesta-6-en-3β-ol (3), (22E)-5α, 8α-epidioxy-choles--6, 22-dien-3β-ol (4), and 5α, 8α-epidioxy-choles--6-en-3β-ol (5). These compounds showed different degrees of antimicrobial activities in bioassay in vitro. Conclusion: The five compounds have been identified for the first time from the gorgonian Muriceopsis flavida. Compounds 2 shows a strong anti-algae effect and is worth further studying.