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Objective To investigate the effect of Fuxin tablet on thrombus formation in the arteries in rats and aggregation of platelet in rabbits. Methods To observe the effect of OT in the arteries through electric stimulation of common carotid artery. To observe the aggregation of platelet by using the PRP of the rabbit and ADP as the accelerant. PAG-1, PAG-3, PAG-5 and PAG-M were examined in the normal saline group and high-dose, middle-dose, low-dose groups of Fuxin tablet. Results High-dose and middle-dose groups of Fuxin tablet could prolong OT and decrease the aggregation of platelet. Conclusion Fuxin tablet could significantly inhibit the formation of thrombus in the arteries and maximal aggregation rate of platelet.
RESUMEN
AIM:To investigate the differences between GC-ECD and GC-MS methods which were employed in the determination of 20 organochlorine pesticide residues in Gancaoxin Granule(Radix et Rhizoma glycyrrhizae,zine preparation) and Fuxin Tablet(Folium Crataegi). METHODS: The solid-phase extraction was used for the clean-up of samples,the quartz capillary columns of DB-1701_(MS) and HP-5_(MS) were selected for the separations of target pesticides,ECD and MS were introduced as the detections respectively.N_2(99.99%,ECD) and He_2((99.999%,)MS) were used as carries,a column flow was at 1.0 mL/min,splitless was chosen as injection mode,injection amount was 1 ?L for all instances,the inlet was set at 230 ℃ and ECD at 300 ℃,negative ions-chemical ionization was used as ion source in MS,the source temperature was set at 200 ℃,the electron energy was at 100 eV,and the temperature of transfer line was at 290 ℃;methane was used as reagent gas with a flow of 3.0 mL/min,scan mode was for qualification,SIM mode was for quantitation. RESULTS: Because 20 organochlorine pesticides were impossible to be separated all on single column by an optimal temperature program to present maximum 19 peaks,on HP and column,both heptachlor-epoxide and oxy-chlordane appeared as a peak,while the overlaid peak in DB-1701 column was contributed by eicher pentachlorothioanisole and aldrin in ECD or oxy-chlordane and ?-666 in MS.We selected SIM mode in MS detection with different m/z and abundance to quantify.By a comparison,the residues of 20 organochlorine pesticides in two preparations showed higher in ECD than that in MS,except for hexachlorobenxene,trans-chlordane,pp'-DDE and endrin,the LOQs of 20 insecticides in ECD was commonly lower than that in MS. CONCLUSION: The ECD method has an advantage in sensitivity and precision,but its result on either qualification and quantitation is prone to the interferences of false positive peaks and background;while MS detection has high specificity in qualification and good accuracy in quantitation,but lower sensitivity and precision compared to ECD.Therefore,GC-ECD method for 20 organochlorine pesticide residues is sensitive and of good precision,while GC-MS method is rapid and accurate.