RESUMEN
Objective: To establish the HPLC fingerprint and multicomponents determination of Potentillae Chinensis Herba, and provide a scientific basis for the improvement of its quality standards. Methods: The separation was performed on a chromatographic Diamonsil C18 column (250 mm × 4.6 mm, 5 μm), with acetonitrile-0.1% phosphoric acid as the mobile phase for gradient elution. Volume flow rate was 1.0 mL/min. Column temperature was 25 ℃. Injection was 10 μL and the detection wavelength was 260 nm. The fingerprints of 10 batches of Potentillae Chinensis Herba were established and evaluated by the similarity evaluation system of TCM (version 2004A), which were divided into two categories by clustering analysis. Meanwhile, the content of galic acid, quercetin, and kaempferol was determined. Results: The fingerprint of Potentillae Chinensis Herba was established. There were 12 common peaks in the fingerprint. Galic acid, quercetin, and kaempferol were separated with good linearity relationships (r > 0.999). The average recovery rates of the investigated compounds were 97.44%, 97.64%, and 99.19%, respectively. Conclusion: The established fingerprint and multicomponents determination method of Potentillae Chinensis Herba have strong specificity and good repeatability, which can effectively control the internal quality of Potentillae Chinensis Herba and provide reference for improving the quality evaluation method of Potentillae Chinensis Herba.
RESUMEN
Objective: To establish an HPLC method for the determination of galic acid, mangiferin, paeoniflorin, benzoic acid, and cinnamic acid in Guizhi Shaoyao Zhimu Decotion (GSZD). Methods: The separation was performed on a Zorbax Extend-C18 (150 mm × 4.6 mm, 5 μm) column with the gradient elution of acetonitrile-water; The volumn flow was 1.0 mL/min; The detection wavelength was set at 230 nm. The colunm temperature was maintained at 25℃. Results: Galic acid, mangiferin, paeoniflorin, benzoic acid, and cinnamic acid had good linearity in the ranges of 0.052-0.52 (r = 0.999 8), 0.078-0.78 (r = 0.999 8), 0.336-3.36 (r = 0.999 9), 0.029 6-0.296 (r = 0.999 8), and 0.020 6-0.206 (r = 0.999 9) μg, respectively. The average recoveries of galic acid, mangiferin, paeoniflorin, benzoic acid, and cinnamic acid were 99.73% (1.63%), 99.33% (1.02%), 100.2% (1.79%), 98.9% (1.02%), and 99.64% (1.62%), respectively. Conclusion: This method is simple, reliable, and accurate, and can provide the reference for study on GSZD material basis.
RESUMEN
Objective: To establish a HPLC method for in vivo determination of gallic acid (GA) and protocatechuic acid (PA) in rat plasma, and to study the effect of Sandalwood on the pharmacokinetics of GA and PA. Methods: SD rats were given the water extracts of Choerospondiatis fruit or Choerospondiatis fruit and Sandalwood. The pharmacokinetic parameters of GA and PA were calculated by DAS2. 0 software at different time points after an oral ration of the above extracts. Results: The pharmacokinetic parameters after oral administration of Choerospondiatis fruit extract were as follows: GA: Cmax: (0.112±0.008) mg·L-1, CL/F: (0.132±0.016) L·min-1·kg-1, t1/2β: (69.3±0) min, Tmax: (45.0±0) min; PA: Cmax: (0.550±0.028) mg·L-1, Tmax: (52.0±0) min, t1/2β, (60.7±1.1) min; CL/F: (0.078±0.011) L·min-1·kg-1. The pharmacokinetic parameters after oral ration of Choerospondiatis fruit-Sandalwood extract were as follows: GA: Cmax: (0.187±0.010) mg·L-1, CL/F: (0.094±0.017) L·min-1·kg-1, t1/2β: (69.3±3.3) min, Tmax: (30.0±0) min; PA: Cmax: (1.080±0.066) mg·L-1, Tmax: (45.0±0) min, t1/2β: (69.3±0.2) min, CL/F: (0.011±0.001) L·min-1·kg-1. Conclusion: Oral ration of Choerospondiatis fruit-Sandalwood extract results in earlier plasma peaks of PA and GA, lower clearance rate, and longer half life, indicating Sandalwood can promote the absorption of phenolic compounds in Choerospondiatis fruit.