RESUMEN
A liquid chromatography-quadrupole-time-of-flight mass spectrometer(LC-Q/TOF-MS) based urinary metabolomic approach was employed to assess the toxicity-alleviation effect of Huangqi oral solution(HOs) on cisplatin-exposed rats and explore its possible mechanisms. Rat toxicity model was developed by multiple intraperitoneal injection of low-dose cisplatin, while HOs was orally administrated to rats simultaneously for 16 consecutive days to attenuate or reduce the cisplatin-induced toxicity. 24-hour urine samples on day 18 were collected and analyzed using LC-Q/TOF-MS to obtain the dataset of urinary metabolites. Principal component analysis(PCA) and orthogonal partial least squares-discriminant analysis(OPLS-DA) were employed to assess the quality of the dataset and screen the potential toxicity-alleviation biomarkers. The serum level of rat creatinine and urea nitrogen on day 20 was determined, and the results showed that successive administration of HOs significantly reduced the cisplatin-induced increase of creatinine and urea nitrogen. PCA cluster analysis clearly demonstrated that HOs could partly improve the CDDP-induced abnormality of metabolic profiling. 35 urinary metabolites were finally screened as the potential biomarkers associated with the toxicity-attenuation effect of HOs, according to the combination of the analysis results of OPLS-DA, t-test and fold change analysis. Further metabolic pathway analysis revealed that HOs could restore the metabolic disorders of amino acid, energy and nucleotide, thereby exerted its toxicity-alleviation effect.
RESUMEN
Objective To study a method for the determination of heterophyllin B inTaizishen- Huangqi oral solution.Methods The HPLC system consisted of Phenomenex Luna-C18 (4.6 mm × 250 mm, 5μm) column, the mobile phase consisted of acetonitrile and 0.1% acetic acid, the gradient elution flow rate was 1.0 ml/min, the column temperature was 30℃, and the UV detector was set at 203 nm.Results The linear response range was 0.016-0.320 mg/ml (r=0.999 8). The average recovery of heterophyllin B was 98.92%, and RSD was 0.80%. The average determination of Heterophyllin B within 5 batches of preparation was 1.63 mg/ml. Conclusions The method showed its high sensitivity, accurate, repeatable and high secificity. It could be used as a method to control the standard ofTaizishen-Huangqi oral solution.