Study on chemical constituents from sponge-derived fungus Penicillium sp. HPQJ10 / 中草药

Objective: To study the secondary metabolites of sponge-derived fungus Penicillium sp. HPQJ10. Methods: The compounds were isolated and purified by chromatographic methods on silica gel, Sephadex-LH20 columns and semi-prep HPLC. The structures were established on the basis of spectroscopic data interpretation. Results: Forteen compounds were isolated and identified as N-[(Z)-2-(4-hydroxyphenyl) ethenyl] formamide (1), 2,4-dimethoxyphenyl acetate (2), 6,8,5′6′-tetrahydroxy-3′- methylflavone (3), 4-hydroxyphenylacetic acid (4), cyclo-(L-Pro-L-Val) (5), cyclo-(L-Pro-L-Leu) (6), diisobutyl phthalate (7), dibutyl phthalate (8), 7-hydroxy-2-(2-hydroxyPropyl)-5-methyl chromone (9), 2-(2,4-dimethoxylphenyl)-3-(4-hydroxybenzyl)-1,2,4-triazole (10), (22E,24R)-ergenol-5,7,22-trien-3β-ol (11), cyclo-(Leu-Pro-Ile-Pro) (12), 2,4-dimethoxybenzoic acid (13), and 3-methoxyphenol (14). Conclusion: Except for compounds 1, 3, 10, and 11, other compounds are isolated from Penicillium sp. for the first time.

Isolation,Identification and Bioactivity Screening of Streptomyces pseudogriseolus Associated with Marine Sponge Hymeniacidon perleve / 微生物学通报

An actinomycete B37 was isolated from an intertidal marine sponge Hymeniacidon perleve, which has strong activity against Gram positive bacteria and moderate activity against tumor cells. The mycelium and spore morphology, physiological properties and 16SrDNA sequence suggested that B37 is Streptomyces pseudogriseolus. The fermentation conditions of this strain were investigated for the biosynthesis of bioactive metabolites.

Potential of Co-culture Induction in Antibiotics Discovery from Bacteria-associated with Marine Sponge Hymeniacidon perleve / 微生物学通报

Ten actinomycetes strains from inter-tidal marine sponge Hymeniacidon perleve of China Yellow Sea were isolated and bio-assayed for antibiotic activities.3 of the 10 strains displayed antimicrobial activity when in monoculture.While strain Hp-053 was co-cultured with each of the other 9 strains,5 of the 9 co-cultures exhibited enhancing antimicrobial activity.TLC results and bio-autography of extracted samples indicated that chemical compounds and antimicrobial compounds produced by co-cultures of sponge associated actinomycetes were different from those by monoculture.Co-culture induction may be a valuable novel tool for the exploitation of sponge associated microorganism.