Comparison between Conventional Gastric Adenocarcinoma and EBV Associated Gastric Carcinoma

Epstein Barr Virus (EBV or Human herpesvirus 4) belongs to the genus Lymphocryptoviridae, the gamma 1 subtype of the Subfamily Gamma herpes viridae and is one of the most common viruses in humans. It is present in all populations, infecting more than 95% of all individuals within the first four decades of life. In developing countries, infections occur very early in life with no specific characteristics other than the general symptoms of acute viremia. In developed countries however, the infection is usually delayed until adolescence or early childhood years where it causes infectious mononucleosis, a benign self-limiting lymphoproliferative disorder.Though the infection with EBV is benign in the acute stages and latent in the chronic phase in the vast majority of people, the virus has been demonstrated to be involved in the development of many malignancies with the list of such malignancies progressively increasing. The first association was with the endemic Burkitt’s lymphoma. Subsequently, other lymphomas (subtypes of Hodgkin’s and non-hodgkin’s lymphomas) are also known to be associated with EBV infection. Epithelial malignancies such as lymphoepitheliomas of nasopharynx and stomach are included in the list of EBV associated tumors. Tumors arise as a result of genetic and epigenetic alterations produced by the virus, which transforms the normal cell into an immortalized proliferating cell. Since Burke et al first detected EBV in undifferentiated lymphoepithelioma like gastric cancer in 1990, many researches are undertaken to prove the same. EBV expresses latent membrane protein which can be detected immune histochemically.Our study is aimed at detecting the EBV expression in gastric carcinoma cells

Effect of Chinese herb Suifukang on expression of NoGo-A protein in the brain of MACO rat / 中国药学杂志

OBJECTIVE: To study the effect of Chinese herb Suifukang on the expression of Nogo protein-A in the brain of MCAO rats and explore the action mechanism of its promoting neurogenesis. METHODS: SD rats were selected and randomized into six groups: normal group, control group, treatment group of methylprednisolone, treatment group of Chinese herb Suifukang in large-does, middle-does and small-does. The models of MCAO rats were successfully established by the method of Koizumi. After the surgery, the rats of drug group had been taken orally Suifukang by 10 g · kg-1 crude drug one time daily, and the rest groups had been taken orally equivalence saline once a day. The brain of rats were investigated and the expression of NoGo-A was detected by immunohistology method and RT-PCR method. RESULTS: The expression of NoGo-A in the injured brain was apparently inhibited by Suifukang (P < 0.01). CONCLUSION: Suifukang can inhibit the expression of NoGo-A. So this function may be one of the mechanisms that Suifukang can promote the functional recovery after brain injury. Copyright 2013 by the Chinese Pharmaceutical Association.

Inhibitory effect of traditional Chinese medicine Suifukang on the expression of chondroitin sulfate proteoglycans in cerebral ischemia zone of rats and glia scar cultured in vitro / 中国药学杂志

OBJECTIVE: To study whether the traditional Chinese medicine Suifukang can inhibit the expression of chondroitin sulfate proteoglycans(CSPGs) in rats with middle cerebral artery occlusion. METHODS: SD rats were selected and randomized into six groups: normal group, control group, methylprednisolone treatment group, and Suifukang high dose, middle dose and low dose treatment groups. Serum pharmacological method was used to prepare serum containing different concentrations of Suifukang. And glial scar model established in vitro was treated with the drug-containg serum. The expression of CSPGs was detected by immunohistology method. RESULTS: The expression of CSPGs in the injured brain and glial scar in vitro were significantly inhibited by Suifukang (P < 0.01). CONCLUSION: Suifukang can inhibit the expression of CSPGs. This function may be one of the mechanisms that Suifukang promotes the regeneration of axon and the functional recovery after ischemical injury of brain.

Analysis of the expression of bcl-2 in osteogenic tumor and tumor-diseases / 临床外科杂志

Objective To investingate the expression method of bcl-2 and it's significance in osteogenic tumor and tumor-like diseases.Method The protein of PCNA, p53, Fas and bcl-2 were examined immunohistologically in osteosarcoma, ossifying fibroma, fibeous dysplasia of bone and normal bone callus. The results were analysed combing with the information of metastasis and hisomorphology.Results PCNA and p53 were highly expressed in low-differentiated osteosarcoma, bcl-2 were highly expressed at high-differentiated osteocoma and ossifying fibroma. When PCNA, p53 and bcl-2 were all expressed at high level, the metastasis rate was high, when only bcl-2 was expressed at high level, the progmosis was better. Conclusion The mechanism for benign and malignant tumor are different, the benign and low-degree malignant tumor may be caused by over accumulated mutation cells sipported by high level of bcl-2, while malignant tumor may be the result of multi-gene cooperation abnormally.

Evaluation of Vascular Smooth Muscle Cell Lineages Derived from the Transgenic Mice Expressing Temperature-Sensitive Simian Virus 40 T Antigen Targeted to Smooth Muscle

BACKGROUND AND OBJECTIVES: As a part of efforts to evaluate effectiveness of aorta cell lineages derived from the transgenic mice in which expression of the temperature-sensitive SV40 large T antigen has been targeted to vascular smooth muscle, localization of the T antigen gene in chromosome of the established cell lineages was characterized. Expression pattern of p53 in an aorta cell line or those of r-actin and the T antigen in the various tissues of the transgenic mice, respectively, were also examined. MATERIALS AND METHODS: Chromosomal DNA obtained from the aorta cell lines, which were derived from the transgenic mice, was analyzed by genomic Southern blot method to identify the transgene in genome. mRNA was prepared from the various tissues of the transgenic mouse and was examined by Northern blot analysis to detect expression of r-actin gene. Reverse transcription polymerase chain reaction (RT-PCR) was also performed to examine transcription pattern of p53 gene in an aorta cell line. Immunohistology for detecting the T antigen expression in the tissues of the transgenic mouse was also carried out. RESULTS: Correct integration of the SV40 T antigen transgene in chromosome was observed in two aorta cell lines, although their copy numbers inserted were different from each other. Alternative splicing of the primary p53 RNA was identified in the aorta cells when cultured at the restrictive temperature, but not in the cells at permissive temperature. Several tissues of the transgenic mouse showed expression of the viral oncoprotein T antigen. CONCLUSION: An established aorta cell line may be useful model to study the mechanism regulating the proliferation of vascular smooth cells in mice. Mutant form (s) of the p53 tumor suppressor protein generated by the translation of the alternatively spliced mRNA might be involved in the blockade of apoptosis in some aorta cells when cultured at the restrictive temperature. However, expression of the viral gene in the tissues of the transgenic mice seemed not to be precisely regulated by temperature-dependent manner.

Localization Forms and Clinical Implication of HBsAg、HBcAg in Liver Tissue with Chronic Hepatitis B / 昆明医科大学学报

The study reports on HBsAg and HBcAg in liver tissue samples of 36 patients with chronic hepatitis B were detected with ABC method. Among them 12 cases were HBsAg and/or HBcAg positive (32.4%). We found a high positive rate of replication signs of HBV with chronic active hepatitis indicating active replication of HBV. Also the extent of HBV replication was found parallel to that of active hepatopathy. Those with strong positive stain- ing always have middle or heavy chronic active hepatitis. In our studies active HBV replication and hepatopathy were observed. HBsAg in the liver mainly showed spread form of distribution and HBcAg mainly showed nuclear and cytoplasmic distribution, which was con- sistent with relevant publications. These results indicate that HBV replication is relevant to hepatopathy and its activity