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1.
Cancer Research and Treatment ; : 80-89, 2019.
Artículo en Inglés | WPRIM | ID: wpr-719716

RESUMEN

PURPOSE: In the presence of interferon, proteasome subunits are replaced by their inducible counterparts to form an immunoproteasome (IP) plays a key role in generation of antigenic peptides presented by MHC class I molecules, leading to elicitation of a T cell‒mediated immune response. Although the roles of IP in other cancers, and inflammatory diseases have been extensively studied, its significance in breast cancer is unclear. MATERIALS AND METHODS: We investigated the expression of LMP7, an IP subunit, and its relationship with immune system components in two breast cancer cohorts. RESULTS: In 668 consecutive breast cancer cohort, 40% of tumors showed high level of LMP7 expression, and tumors with high expression of LMP7 had more tumor-infiltrating lymphocytes (TILs) in each subtype of breast cancer. In another cohort of 681 triple-negative breast cancer patients cohort, the expression of LMP7 in tumor cells was significantly correlated with the amount of TILs and the expression of interferon-associated molecules (MxA [p < 0.001] and PKR [p < 0.001]), endoplasmic reticulum stress-associated molecules (PERK [p=0.012], p-eIF2a [p=0.001], and XBP1 [p < 0.001]), and damage-associated molecular patterns (HMGN1 [p < 0.001] and HMGB1 [p < 0.001]). Patients with higher LMP7 expression had better disease-free survival outcomes than those with no or low expression in the positive lymph node metastasis group (p=0.041). CONCLUSION: Close association between the TIL levels and LMP7 expression in breast cancer indicates that better antigen presentation through greater LMP7 expression might be associated with more TILs.


Asunto(s)
Humanos , Presentación de Antígeno , Neoplasias de la Mama , Mama , Estudios de Cohortes , Supervivencia sin Enfermedad , Retículo Endoplásmico , Antígenos HLA , Proteína HMGB1 , Sistema Inmunológico , Interferones , Ganglios Linfáticos , Linfocitos Infiltrantes de Tumor , Metástasis de la Neoplasia , Péptidos , Complejo de la Endopetidasa Proteasomal , Neoplasias de la Mama Triple Negativas
2.
Rev. Soc. Bras. Med. Trop ; 50(1): 99-103, Jan.-Feb. 2017. graf
Artículo en Inglés | LILACS | ID: biblio-1041391

RESUMEN

Abstract: INTRODUCTION: Infection with all serotypes of dengue virus (DV) results in augmented antigen presentation by MHC class I molecules. However, the upregulation of immunoproteasome subunits only results from infection with two serotypes. This study aims to elucidate changes in the expression of immunoproteasome subunits resulting from infection with DV, particularly DV serotype 2 (DV2). METHODS: HepG2 cells were grown in various culture milieu. Total cellular RNA and proteins were extracted and quantified. RESULTS: Results demonstrated sequestration of immunoproteasome subunits LMP2 and LMP7 in DV2-infected cells. CONCLUSIONS: This study provides insights into the mechanisms underlying immune evasion by DV.


Asunto(s)
Humanos , Virus del Dengue/metabolismo , Complejo de la Endopetidasa Proteasomal/metabolismo , Regulación de la Expresión Génica , Subunidades de Proteína , Virus del Dengue/clasificación , Células Hep G2 , Serogrupo
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