Genetic diversity analysis on rhizosphere soil microbial population of Panax ginseng and Panax quinque folium by RAPD / 中草药

Objective: To study rhizosphere and non-rhizosphere soil microbial population genetic diversity of Panax ginseng and P. quinquefolium with different growing years. Methods: Eighteen P. ginseng and P. quinquefolium rhizosphere and non-rhizosphere soil samples were collected from Fusong county of Jilin Province, and genetic diversity of microbial population construction were analyzed by RAPD. Results: Cultivation times have great influence on microbial population construction of rhizosphere and non-rhizosphere soil. Also we found that there were differences between soil microbial population construction of P. ginseng and P. quinquefolium. Furthermore, rhizosphere effect of plants on soil microbial population construction was also found. Conclusion: Directional selection pressure that root secretions of P. ginseng and P. quinquefolium to soil microorganisms is one of the main drives that resulted in the change of microbial population genetic diversity, and microbial population construction change in P. ginseng and P. quinquefolium cultivated soil is the key factor for soil ecological function turbulence and the development of continuously cropping obstacle.

Apoptosis Expression-based Pattern Recognition System for Four Natures of Chinese Materia Medica——Comparative Study on Drug Natures of the Water Extracts of Panax ginseng,Dry Panax ginseng and Panax quinque folium against A?_(25-35)-induced Apoptosis of / 中国药房

OBJECTIVE:To investigate the effects of water extracts of Panax ginseng(warm),dry P.ginseng(mild),P.quinque folium(cool) on the apoptosis of SH-SY5Y cells induced by ?-amyloid protein(A?25-35).METHODS:SH-SY5Y cells were treated with A?25-35 to simulate the pathologic neuronic damage model in patients with Alzheimer's disease,and then treated with different doses of water extracts of P.ginseng(WERG),dry P.ginseng(WEG)or P.quinque folium(WEAG).The morphologic changes of the SH-SY5Y cells were observed by inverted microscope.Cell survival rate was measured by MTT assay.The percentage of apoptosis and the expression of Bax and Bcl-2 proteins were detected by Western Blotting.RESULTS: After treatment with A?25-35 50 ?mol?L-1 for 72 hours,SH-SY5Y cells became more round and aggregated and the cell survival rate was significantly reduced(P

Apoptosis Expression-based Pattern Recognition System of Four Properties of Chinese Materia Medica——Panax quinque folium vs. Panax ginseng in Drug Property against MPP~+-induced Apoptosis of SH-SY5Y Cells / 中国药房

OBJECTIVE:To investigate the effects of water extract of Panax quinque folium(WEAG,of cool property)and Panax ginseng(WERG,of warm property)on MPP+-induced apoptosis of SH-SY5Y cells.METHODS:SH-SY5Y cells were divided into 4 groups:the control group was cultured in complete medium,model group was cultured with 0.5 mmol MPP+;Panax quinque folium group was treated with 0.5 mmol MPP+ plus different concentration of WEAG,and Panax ginseng group was treated with 0.5 mmol MPP+ and different concentration of WERG concomitantly.WEAG and WERG were added for intervention.the morphology of SH-SY5Y cells was observed by Hoechst33258 staining and the apoptotic rate was detected by FCM;the cell survival rate was measured by MTT chromatometry.RESULTS:Compared with model group,WEAG and WERG markedly reversed the apoptotic characteristics of SH-SY5Y cells,markedly up-regulated MTT value and lowered the apoptotic rate,with WERG showing more potent efficacy than WEAG doing.CONCLUSION:Water extracts of both Panax quinque folium(cool)and Panax ginseng(warm)pose protective effects of different degree against MPP+-induced apoptosis of SH-SY5Y cells,with the Chinese materia medica of warm property showing more potent action against apoptosis.Chinese materia medicas of different drug property have different anti-apoptotic action.